Routine use of real-time PCR for detection of Helicobacter pylori and of clarithromycin resistance mutations

Tankovic, Jacques; Chaumette-Planckaert, Marie-Thérèse; Deforges, Lionel; Launay, Nathalie; Glaunec, Jeanne-Marie Le; Soussy, Claude-James; Delchier, Jean‐Charles

doi:10.1016/s0399-8320(07)73967-2

Cited by 16 publications

(5 citation statements)

References 18 publications

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“…Real‐time PCR performances were also favorable in comparison to histology (sensitivity 98.2 vs. 87.7%) in a study by Tankovic et al. [18]. A nested PCR targeting Hsp60 was found of interest for H. pylori diagnosis [19].…”

Section: Invasive Testsmentioning

confidence: 99%

Diagnosis of Helicobacter pylori

et al. 2008

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The different invasive and noninvasive diagnostic tests for Helicobacter pylori have been applied mainly in emerging countries. Molecular methods have been developed, especially a test for detection of H. pylori and its clarithromycin resistance directly from stools. The long-term effects of eradication on histologic lesions have been studied in a meta-analysis and the prognostic value of post-treatment in gastric mucosa-associated lymphoid tissue lymphoma has been assessed. An operating link for gastritis assessment (the OLGA staging) has also been published. Attempts to simplify the urea breath test protocol have been made, and new stool antigen tests have been proposed and compared to those previously available.

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Section: Invasive Testsmentioning

confidence: 99%

Diagnosis of Helicobacter pylori

et al. 2008

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“…Compared to other available methods for diagnosing H. pylori for clinical and research purposes, PCR yields high sensitivity and specificity for H. pylori in frozen samples from patients with nonbleeding peptic ulcer [7], [8]. PCR is also far better than histology at detecting H. pylori in fresh frozen gastric biopsies from PUB patients [9].…”

Section: Introductionmentioning

confidence: 99%

Real-Time PCR Improves Helicobacter pylori Detection in Patients with Peptic Ulcer Bleeding

et al. 2011

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Background and AimsHistological and rapid urease tests to detect H. pylori in biopsy specimens obtained during peptic ulcer bleeding episodes (PUB) often produce false-negative results. We aimed to examine whether immunohistochemistry and real-time PCR can improve the sensitivity of these biopsies.Patients and MethodsWe selected 52 histology-negative formalin-fixed paraffin-embedded biopsy specimens obtained during PUB episodes. Additional tests showed 10 were true negatives and 42 were false negatives. We also selected 17 histology-positive biopsy specimens obtained during PUB to use as controls. We performed immunohistochemistry staining and real-time PCR for 16S rRNA, ureA, and 23S rRNA for H. pylori genes on all specimens.ResultsAll controls were positive for H. pylori on all PCR assays and immunohistochemical staining. Regarding the 52 initially negative biopsies, all PCR tests were significantly more sensitive than immunohistochemical staining (p<0.01). Sensitivity and specificity were 55% and 80% for 16S rRNA PCR, 43% and 90% for ureA PCR, 41% and 80% for 23S rRNA PCR, and 7% and 100% for immunohistochemical staining, respectively. Combined analysis of PCR assays for two genes were significantly more sensitive than ureA or 23S rRNA PCR tests alone (p<0.05) and marginally better than 16S rRNA PCR alone. The best combination was 16S rRNA+ureA, with a sensitivity of 64% and a specificity of 80%.ConclusionsReal-time PCR improves the detection of H. pylori infection in histology-negative formalin-fixed paraffin-embedded biopsy samples obtained during PUB episodes. The low reported prevalence of H. pylori in PUB may be due to the failure of conventional tests to detect infection.

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“…L'histologie classique devrait être remplacée à court terme par la Pcr [3] ou le test Helico-Dr ® [4] qui permettent à la fois de faire le diagnostic de l'infection et des mutations liées aux résistances à clarithromycine et aux quinolones. Le test respiratoire à l'urée c13 n'est utilisable en dépistage primaire que chez les apparentés de cancer gastrique de moins de 40 ans et chez les malades devant être mis aux anti-inflammatoires non stéroïdiens.…”

Section: Quelles Sont Les Méthodes De Recherche De L'infection H Pylunclassified

Quand rechercher Helicobacter pylori et comment l’éradiquer: techniques de recherche, traitements, vérification de l’éradication

Delchier

2011

Acta Endosc

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Routine use of real-time PCR for detection of Helicobacter pylori and of clarithromycin resistance mutations

Cited by 16 publications

References 18 publications

Diagnosis of Helicobacter pylori

Diagnosis of Helicobacter pylori

Real-Time PCR Improves Helicobacter pylori Detection in Patients with Peptic Ulcer Bleeding

Quand rechercher Helicobacter pylori et comment l’éradiquer: techniques de recherche, traitements, vérification de l’éradication

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