Rouxiella chamberiensis gen. nov., sp. nov., a member of the family Enterobacteriaceae isolated from parenteral nutrition bags

Flèche‐Matéos, Anne Le; Levast, Marion; Lomprez, Fabienne; Arnoux, Yolande; Andonian, Clément; Perraud, M.; Vincent, Véronique; Gouilh, Meriadeg Ar; Thiberge, Jean‐Michel; Diancourt, Laure; Caro, Valérie; Burguière, Ana Maria; Manuguerra, Jean-Claude

doi:10.1099/ijs.0.000179

Cited by 32 publications

(14 citation statements)

References 19 publications

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“…Finally, R. silvae is different from R. chamberiensis in that it is unable to grow at 37 C, to reduce nitrate, or to ferment trehalose, rhamnose, and maltose sugars. The GC content of its DNA is also different [14]. Therefore, the bacterial strain isolated in this research is identified as R. chamberiensis, and its asparaginase production activity is reported for the first time.…”

Section: Discussionmentioning

confidence: 95%

L-Asparaginase-producing Rouxiella Species Isolation, Antileukemia Activity Evaluation, and Enzyme Production Optimization

Gilavand

Kavyanifard

Marzban

2019

RMM

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Background: L-Asparaginase (L-Asp) is used as an efficient anti-cancer drug, especially for acute lymphoblastic leukemia (ALL). Currently, two bacterial asparaginase isoenzymes are used for cancer treatment. Therefore, this research focused on isolating native bacteria with the ability to produce L-Asp. Materials and Methods: L-Asp producing bacteria were isolated from soil samples on 9K medium supplemented with L-Asp as nitrogen source. Detection of L-Asp activity was performed by observing color change of the agar medium from yellow to orange due to the release of ammonia around the colonies. After the isolation and identification of the bacterium, L-Asp production was first optimized by the one factor-at-the-time (OFAT) technique followed by the response surface method. Next, the enzyme was extracted, purified, and assessed for antileukemia activity on U937 and MRC-5 cell lines. Results: The results revealed that L-Asp produced by Rouxiella sp. AF1 significantly inhibited the growth of U937 cells at a dose of up to 0.04 IU/ml, while MRC-5 was not affected at any enzyme doses. The final purification of the enzyme was achieved by column chromatography (Sephadex G-100) at approximately 0.31 mg/ml, and its specific activity was determined to be 0.51 IU/mg. The OFAT optimization experiments were performed primarily to determine optimal enzyme conditions, which were found to be neutral pH (pH7), 30 ∘C temperature, and 3 % NaCl, 1 % peptone, and 1% glucose concentrations. Statistical optimization was based on five factors obtained from OFAT, and response surface method (RSM) analysis introduced a quadratic model for enzyme production at the optimal range of these variables. This model provided an equation for measuring the effect of physiochemical conditions on final enzyme production. Conclusion: We showed that native bacteria may be novel candidates for isolating new metabolites such as L-Asp. Because many bacteria grow in unknown environments with unique ecological properties, the probability of discovering novel bacterial species producing bioactive compounds is high.

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Section: Discussionmentioning

confidence: 95%

L-Asparaginase-producing Rouxiella Species Isolation, Antileukemia Activity Evaluation, and Enzyme Production Optimization

Gilavand

Kavyanifard

Marzban

2019

RMM

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“…The genera Coetzeea and Thorsellia, are the only genera described for the Thorselliaceae family. Even though this review focuses only on genera within the family Enterobacteriaceae described during the 21th century, Table 1 shows all the families and genera that belong to the order Enterobacteriales [13][14][15][16][17][18][19][20][21] , in order to show the genera related to Enterobacteriaceae.…”

Section: Resultsmentioning

confidence: 99%

Enterobacteria in the 21st century: a review focused on taxonomic changes

Morales-López

Yepes

Prada-Herrera

et al. 2019

J Infect Dev Ctries

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Introduction: Enterobacteria are the main group causing infections in humans. The aim of this review is to present the new genera and the taxonomic changes that the Enterobacteriacea family has experienced in recent years. Methodology: a systematic search of papers published in databases from January 2000 to July 2018 was done. Additionally, the bibliographic references of each document were reviewed and each paper citing the article was reviewed in search of clinical cases. Results: Nineteen new genera of Enterobacteria have been described since 2000. The genera Yersinia, Morganella and Erwinia do not belong to the family Enterobacteriacea anymore. Conclusions: for an adequate clinical and epidemiological interpretation, it is advisable to update the libraries of the commercial systems used for the identification of the microorganisms, as well as to train the staff in the taxonomic changes of microorganisms.

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“…Some Rahnella species, such as R. aquatilis found in freshwater and soil, have been associated with infections in humans, usually immunocompromised and trauma patients 35 , 36 . The next closest identity (98.7%, 1398/1417 bp) was to Rouxiella chamberiensis , which was described from a case involving infant mortalities due to contaminated parenteral nutrition bags 37 . Sequence identity to other bacterial organisms was significantly lower, at 98% and below and mainly to Hafnia and Serratia species.…”

Section: Resultsmentioning

confidence: 99%

El Niño drives a widespread ulcerative skin disease outbreak in Galapagos marine fishes

Lamb

Smith

Aued

et al. 2018

Sci Rep

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Climate change increases local climatic variation and unpredictability, which can alter ecological interactions and trigger wildlife disease outbreaks. Here we describe an unprecedented multi-species outbreak of wild fish disease driven by a climate perturbation. The 2015–16 El Niño generated a +2.5 °C sea surface temperature anomaly in the Galapagos Islands lasting six months. This coincided with a novel ulcerative skin disease affecting 18 teleost species from 13 different families. Disease signs included scale loss and hemorrhagic ulcerated patches of skin, fin deterioration, lethargy, and erratic behavior. A bacterial culture isolated from skin lesions of two of the affected fish species was identified by sequencing of the 16S rRNA gene as a Rahnella spp. Disease prevalence rates were linearly correlated with density in three fish species. In January 2016, disease prevalence reached 51.1% in the ring-tailed damselfish Stegastes beebei (n = 570) and 18.7% in the king angelfish Holacanthus passer (n = 318), corresponding to 78% and 86% decreases in their populations relative to a 4.5-year baseline, respectively. We hypothesize that this outbreak was precipitated by the persistent warm temperatures and lack of planktonic productivity that characterize extreme El Niño events, which are predicted to increase in frequency with global warming.

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Rouxiella chamberiensis gen. nov., sp. nov., a member of the family Enterobacteriaceae isolated from parenteral nutrition bags

Abstract: Rouxiella chamberiensis gen. nov., sp. nov., a member of the family Enterobacteriaceae isolated from parenteral nutrition bags

Cited by 32 publications

References 19 publications

L-Asparaginase-producing Rouxiella Species Isolation, Antileukemia Activity Evaluation, and Enzyme Production Optimization

L-Asparaginase-producing Rouxiella Species Isolation, Antileukemia Activity Evaluation, and Enzyme Production Optimization

Enterobacteria in the 21st century: a review focused on taxonomic changes

El Niño drives a widespread ulcerative skin disease outbreak in Galapagos marine fishes

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