Roy, Arundhati (1961–)

Bakshi, Sandeep

doi:10.4135/9781412956215.n753

Cited by 1 publication

(1 citation statement)

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“…Students also collect and process seawater samples to gain hands-on field experience and a greater understanding of the scientific process. This mCURE curriculum can be adapted to match the flexible framework indicated in Bakshi et al 2016 (4) and can also be utilized in series with other published courses on high throughput dilution-based isolation of bacteria (16) or the genomic characterization of bacterial isolates (17). This mCURE course has been field-tested with 147 students with the following marine bacterioplankton isolates: Gammaproteobacteria bacterium sp.…”

Section: Introductionmentioning

confidence: 99%

A CURE for physiological characterization of bacterioplankton in liquid culture

Lanclos

Coelho

Hyer

et al. 2020

Preprint

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Bacterial characterization is an important aspect of microbiology that includes experimentally determining growth rates, environmental conditions conducive to growth, and the types of energy sources microorganisms can use. Researchers use this information to help understand and predict an organism's ecological distribution and environmental functions. Microbiology students generally conduct bacterial characterization experiments in their coursework; however they are frequently restricted to model organisms without ecological relevance and for which the results have been known for decades. We present a course-based undergraduate research experience (CURE) curriculum to involve students in characterization of previously untested, ecologically relevant bacterioplankton cultures to identify the carbon substrates used for growth, as well as the temperature and salinity ranges conducive to growth. Students use these results to connect their organism's physiology to the isolation environment. This curriculum also exposes students to advanced microbiology methods such as flow cytometry for measuring cell concentrations, teaches them to use the programming language R for data plotting, and emphasizes scientific communication through writing, speaking, poster creation/presentation, and social media. This CURE is an attractive introduction to scientific research and was successfully tested with 147 students during the fall semester of 2018.

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Section: Introductionmentioning

confidence: 99%