RP-HPLC analysis of manool-rich Salvia officinalis extract and its antimicrobial activity against bacteria associated with dental caries

Moreira, Monique Rodrigues; Souza, Ariana B.; Moreira, Maisa A.; Bianchi, Thamires Chiquini; Carneiro, Luiza J.; Estrela, Fernanda T.; Santos, Raquel; Januário, Ana Helena; Martins, Carlos Henrique Gomes; Ambrósio, Sérgio Ricardo; Veneziani, Rodrigo Cássio Sola

doi:10.1590/s0102-695x2013000600003

Cited by 25 publications

(25 citation statements)

References 28 publications

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“…Moreover, the robustness of the method was assessed as previously described [5] and satisfactorily met the requirements of the main Brazilian regulatory agency [29].…”

Section: Resultsmentioning

confidence: 99%

“…The expressions LOD = 3.3σ/S and LOQ = 10σ/S were employed and leaded to respective LOD and LOQ values of 0.267 μg·mL −1 and 0.809 μg·mL −1 , which are considered adequate for the purposes of the studies. The precision, accuracy, and robustness of the method were assessed as previously described [5]; quantitative results, expressed as the relative amount of KA (% w/w) in KAMg, are reported as 95% confidence intervals.…”

Section: Characterization Of Kamgmentioning

confidence: 99%

“…The use of medicinal plant extracts to prevent dental caries has received great attention [3]; indeed, many studies have reported on the ability of medicinal plant extracts to inhibit oral pathogen growth (or adhesion to surfaces) and reduce dental plaque [1,4,5]. Moreover, the addition of such extracts to oral products like mouthwashes and dentifrices constitutes an important strategy in caries control [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, our research group has pointed out the antimicrobial potential of some subclasses of these metabolites against several oral pathogens: pimaranes [9,10,11], kauranes [12,13], labdanes [5,14,15], and abietanes [16]. Among all the investigated compounds, ent-kaurenoic acid (KA; Fig.…”

Section: Introductionmentioning

confidence: 99%

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ent-Kaurenoic acid-rich extract from Mikania glomerata: In vitro activity against bacteria responsible for dental caries

et al. 2016

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Many studies have reported that medicinal plant extracts can inhibit oral pathogen growth or adhesion to surfaces and therefore reduce dental caries formation. The addition of these extracts to oral products like mouthwashes and dentifrices is considered an important strategy in caries control. In this sense, we have developed a Mikania glomerata extract with high ent-kaurenoic acid content (KAMg). So, this work describes the preparation of such extract and the development of a validated HPLC-DAD method to determine its ent-kaurenoic acid (KA) content. Herein it is also described the KAMg in vitro antibacterial evaluation against several cariogenic bacteria in comparison with KA and the investigation of further aspects of the KAMg activity. Toxicological aspects of the developed extract were evaluated by assessing its cytotoxicity and genotoxicity. KA and a KA-rich extract like KAMg showed to inhibit the growth of microorganisms responsible for dental caries at relatively low MIC (Minimum inhibitory concentration) values, albeit not as low as the MIC value obtained for chlorhexidine digluconate (CHD), the golden anticariogenic standard approved by the American Dental Association Council on Dental Therapeutics. However, KAMg was more effective to inhibit the formation of a Streptococcus mutans biofilm with four times lower MICB50 (minimum inhibitory concentration that reduces 50% of the biofilm) value as compared with CHD. Taking into account all these data and considering the absence of genotoxic and cytotoxic activity under the tested conditions, it is suggested that KAMg is a natural product to be considered as active ingredient in oral care products.

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“…Moreover, the robustness of the method was assessed as previously described [5] and satisfactorily met the requirements of the main Brazilian regulatory agency [29].…”

Section: Resultsmentioning

confidence: 99%

Section: Characterization Of Kamgmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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ent-Kaurenoic acid-rich extract from Mikania glomerata: In vitro activity against bacteria responsible for dental caries

et al. 2016

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“…Stereoselective access to manoyl oxide stereoisomers is aprerequisite for the biotechnological production of forskolin derivatives with novel stereochemical configurations.I nc ombination with the class II diTPS Tw TPS21 from Tripterygium wilfordii, CfTPS3 catalyzed the stereoselective formation of (13R) [8] Thef ormation of structurally distinct bicyclic diterpenes, such as sclareol, which is used in high-end fragrance manufacture,i sc atalyzed in S. sclarea by SsLPPS and SsSCS. [9] Antibiotic activities have been reported for ac ompound of this class,(+ +)-manool, [10] which is also recognized as as tarting material for the semisynthesis of aw ide range of diterpenoids with pharmaceutical properties. [11] SsSCS was used as ar epresentative of class Id iTPSs that, following rearrangement, facilitate neutralization of the carbocation through water quenching at C13 to give regiospecific introduction of ahydroxy group.…”

mentioning

confidence: 99%

Expanding the Landscape of Diterpene Structural Diversity through Stereochemically Controlled Combinatorial Biosynthesis

Andersen-Ranberg¹,

Kongstad

Nielsen³

et al. 2016

Angewandte Chemie

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Plant-derived diterpenoids serve as important pharmaceuticals,f ood additives,a nd fragrances,y et their low natural abundance and high structural complexity limits their broader industrial utilization. By mimicking the modularity of diterpene biosynthesis in plants,w ec onstructed 51 functional combinations of class Iand II diterpene synthases,41ofwhich are "new-to-nature". Stereoselective biosynthesis of over 50 diterpene skeletons was demonstrated, including natural variants and novel enantiomeric or diastereomeric counterparts. Scalable biotechnological production for four industrially relevant targets was accomplished in engineered strains of Saccharomyces cerevisiae.

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Expanding the Landscape of Diterpene Structural Diversity through Stereochemically Controlled Combinatorial Biosynthesis

Andersen-Ranberg¹,

Kongstad

Nielsen³

et al. 2016

Angew Chem Int Ed

142

198

View full text Add to dashboard Cite

Plant‐derived diterpenoids serve as important pharmaceuticals, food additives, and fragrances, yet their low natural abundance and high structural complexity limits their broader industrial utilization. By mimicking the modularity of diterpene biosynthesis in plants, we constructed 51 functional combinations of class I and II diterpene synthases, 41 of which are “new‐to‐nature”. Stereoselective biosynthesis of over 50 diterpene skeletons was demonstrated, including natural variants and novel enantiomeric or diastereomeric counterparts. Scalable biotechnological production for four industrially relevant targets was accomplished in engineered strains of Saccharomyces cerevisiae.

show abstract

RP-HPLC analysis of manool-rich Salvia officinalis extract and its antimicrobial activity against bacteria associated with dental caries

Cited by 25 publications

References 28 publications

ent-Kaurenoic acid-rich extract from Mikania glomerata: In vitro activity against bacteria responsible for dental caries

ent-Kaurenoic acid-rich extract from Mikania glomerata: In vitro activity against bacteria responsible for dental caries

Expanding the Landscape of Diterpene Structural Diversity through Stereochemically Controlled Combinatorial Biosynthesis

Expanding the Landscape of Diterpene Structural Diversity through Stereochemically Controlled Combinatorial Biosynthesis

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