RP‐HPLC‐DAD for simultaneous estimation of mahanine and mahanimbine in <i>Murraya koenigii</i>

Pandit, Subrata; Kumar, M Siva Selva; Ponnusankar, Sivasankaran; Pal, Buddhadev; Mukherjee, Pulok K.

doi:10.1002/bmc.1561

Cited by 19 publications

(6 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Spectral information was analyzed in the 200–400 nm region, and chromatograms were detected at a wavelength of 280 nm. Based on previous findings, the quantitative quantification of each bioactive component contained in the plant extracts was determined [ 38 , 39 , 40 ]. Peak identification was carried out by comparing the retention times of specific standards with those of the extract.…”

Section: Methodsmentioning

confidence: 99%

Ethnopharmacological Effects of Urtica dioica, Matricaria chamomilla, and Murraya koenigii on Rotenone-Exposed D. melanogaster: An Attenuation of Cellular, Biochemical, and Organismal Markers

et al. 2022

View full text Add to dashboard Cite

Natural antioxidants derived from plants have been proven to have significant inhibitory effects on the free radicals of living organisms during actively metabolization. Excessive production of free radicals increases the risk of neurodegenerative diseases, such as Alzheimer’s disease, Parkinson’s disease, and motor sclerosis. This study aimed to compare the ethnopharmacological effects of Urtica dioica (UD), Matricaria chamomilla (MC), and Murraya koenigii (MK) on the amelioration of rotenone-induced toxicity in wild-type Drosophila melanogaster (Oregon R+) at biochemical, cellular, and behavioral levels. Phytoextracts were prepared from all three plants, i.e., UD, MC, and MK (aqueous and ethanolic fractions), and their bioactive compounds were evaluated using in vitro biochemical parameters (DPPH, ABTS, TPC, and TFC), UV-Vis, followed by FT-IR and HPLC. Third instar larvae and freshly eclosed flies were treated with 500 µM rotenone alone or in combination with UD, MC, and MK for 24 to 120 h. Following exposure, cytotoxicity (dye exclusion test), biochemical (protein estimation and acetylcholinesterase inhibition assays), and behavioral assays (climbing and jumping assays) were performed. Among all three plant extracts, MK exhibited the highest antioxidant properties due to the highest TPC, TFC, DPPH, and ABTS, followed by UD, then MC. The overall trend was MK > UD > MC. In this context, ethnopharmacological properties mimic the same effect in Drosophila, exhibiting significantly (p < 0.05) reduced cytotoxicity (trypan blue), improved biochemical parameters (proteotoxicity and AChE activity), and better behavioral parameters in the organisms cotreated with phyto extracts compared with rotenone. Conclusively, UV-Vis, FTIR, and HPLC analyses differentiated the plant extracts. The findings of this research may be beneficial in the use of select herbs as viable sources of phyto-ingredients that could be of interest in nutraceutical development and various clinical applications.

show abstract

Section: Methodsmentioning

confidence: 99%

Ethnopharmacological Effects of Urtica dioica, Matricaria chamomilla, and Murraya koenigii on Rotenone-Exposed D. melanogaster: An Attenuation of Cellular, Biochemical, and Organismal Markers

et al. 2022

View full text Add to dashboard Cite

show abstract

“…[1920] In present RP-HPLC analysis, chromatogram of the extract showed sharp peak for puerarin at RT 5.21±0.08 min which was comparable with the standard puerarin (RT, 5.20 ±0.01 min) [Figures 2a, b]. The calibration curve of standard puerarin showed good linearity relationship in the specified concentration range (200-1000 μg/ml) with a correlation coefficient ( r 2 ) greater than 0.99.…”

Section: Resultsmentioning

confidence: 62%

A validated RP-HPLC-UV method for quantitative determination of puerarin in Pueraria tuberosa DC tuber extract

Maji¹,

Maity²,

Banerji³

et al. 2012

Pharmaceutical Methods

View full text Add to dashboard Cite

Background:Pueraria tuberosa (Fabaceae) is a well-known medicinal herbs used in Indian traditional medicines. The puerarin is one of the most important bioactive constituent found in the tubers of this plant. Quantitative estimation of bioactive molecules is essential for the purpose of quality control and dose determination of herbal medicines. The study was designed to develop a validated reversed phase high-performance liquid chromatography (RP-HPLC) method for the quantification of puerarin in the tuber extract of P. tuberosa.Materials and Methods:The RP-HPLC system with Luna C18 (2) 100 Å, 250 × 4.6 mm column was used in this study. The analysis was performed using the mobile phase: 0.1% acetic acid in acetonitrile and 0.1% acetic acid in water (90:10, v/v) under column temperature 25°C. The detection wavelength was set at 254 nm with a flow rate of 1 ml/min. The method validation was performed according to the guidelines of International Conference on Harmonization.Results:The puerarin content of P. tuberosa extract was found to be 9.28 ±0.09%. The calibration curve showed good linearity relationship in the range of 200-1000μg/ml (r2>0.99). The LOD and LOQ were 57.12 and 181.26μg/ml, respectively and the average recovery of puerarin was 99.73% ±1.02%. The evaluation of system suitability, precision, robustness and ruggedness parameters were also found to produce satisfactory results.Conclusions:The developed method is very simple and rapid with excellent specificity, accuracy and precision which can be useful for the routine analysis and quantitative estimation of puerarin in plant extracts and formulations.

show abstract

“…21,22 The present study reveals the distribution of irigenin in the different Iris Plant species growing at different altitudes of Kashmir valley. The results showed the highest concentration of irigenin in iris germanica and lowest in iris croceae.…”

Section: Discussionmentioning

confidence: 70%

Quantitative Analysis of Irigenin in the Different Species of Iris Plant by RP- HPLC and its Efficacy Against Different Plant Pathogens

Wani¹,

Bhat²,

Mir³

et al. 2017

View full text Add to dashboard Cite

RP‐HPLC‐DAD for simultaneous estimation of mahanine and mahanimbine in Murraya koenigii

Cited by 19 publications

References 8 publications

Ethnopharmacological Effects of Urtica dioica, Matricaria chamomilla, and Murraya koenigii on Rotenone-Exposed D. melanogaster: An Attenuation of Cellular, Biochemical, and Organismal Markers

Ethnopharmacological Effects of Urtica dioica, Matricaria chamomilla, and Murraya koenigii on Rotenone-Exposed D. melanogaster: An Attenuation of Cellular, Biochemical, and Organismal Markers

A validated RP-HPLC-UV method for quantitative determination of puerarin in Pueraria tuberosa DC tuber extract

Quantitative Analysis of Irigenin in the Different Species of Iris Plant by RP- HPLC and its Efficacy Against Different Plant Pathogens

Contact Info

Product

Resources

About