RP-HPLC Method Development and Validation for Simultaneous Estimation of Prazosin and Polythiazide in Bulk and Pharmaceutical Dosage Form

Panigrahy, Uttam Prasad; Kumari, K. Naga Vishnu; Reddy, T. Ram Mohan; Abbulu, K

doi:10.5958/0974-360x.2020.00321.2

Cited by 5 publications

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“…Fimasartan acts on the kidney's rennin-angiotensin cascade, which begins when renin release from the kidney causes the breakdown of angiotensinogen into angiotensin I. In blocking the AT 1 Literature survey reveals the availability of few methods such as ultraviolet (UV), high-performance liquid chromatography (HPLC), and LC-MS in biological fluids and pharmaceutical dosage forms [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16]. The present method gives the accurate information regarding method development and validation of Fimasartan in bulk and pharmaceutical dosage form using UV detector at 265 nm which is simple, accurate, economic, rapid, reproducible, and sensitive according to procedures and acceptance criteria based on FDA guidelines and recommendations of ICH.…”

Section: Introductionmentioning

confidence: 99%

Stability Indicating Method Development and Validation of Fimasartan by Reverse-Phase High-Performance Liquid Chromatography in Bulk and Pharmaceutical Dosage Form

Sruthi

Panigrahy

2021

Asian J Pharm Clin Res

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Objective: A rapid, sensitive and specific reverse phase High performance liquid Chromatography (RP-HPLC) method was developed for the estimation of Fimasartan in bulk and pharmaceutical dosage form. Method: The RP-HPLC analysis was performed isocratically on a Primacel C18 column (150 mm × 4.6 mm internal diameter, 5 μm particle size) using mobile phase of composition Acetonitrile and 0.1% orthophosphoric Acid in 80:20, v/v proportions with a flow rate of 0.8 ml/min. Results: The analyte was monitered with UV-detector at 265 nm. In the developed method Fimasartan elutes at a typical retention time of 2.4 min. The proposed method is having linearity in the concentration ranging from 5-30 μg/ml of Fimasartan. Conclusion: : The method was statistically validated and had been applied to analysis of the drug in bulk and pharmaceutical dosage form.

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Section: Introductionmentioning

confidence: 99%

Stability Indicating Method Development and Validation of Fimasartan by Reverse-Phase High-Performance Liquid Chromatography in Bulk and Pharmaceutical Dosage Form

Sruthi

Panigrahy

2021

Asian J Pharm Clin Res

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Development and Validation of UV Spectrophotometric Method for Determination of Prazosin Hydrochloride

Bharathi,

Sundararajan

2024

J Appl Spectrosc

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Stability Indicating and Simultaneous Determination of Saxagliptin, Dapagliflozin and Metformin in Tablet dosage form using RP-HPLC

Kandula

Sundararajan

2021

RJPT

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Aim: To Perform Simultaneous Determination of Saxagliptin, Dapagliflozin and Metformin Tablet dosage form developed in a simple, Accurate, precise manner. Method: Agilent C18 150 x 4.6mm, 5m. Mobile phase containing 0.1% OPA: Acetonitrile taken in the ratio 50:50 was pumped through column at a flow rate of 1.0 ml/min used for the development of chromatogram. Buffer used in this method was 0.1% OPA. Temperature was maintained at 30°C. Optimized wavelength selected was 260nm. Results and Conclusion: Retention time of Saxagliptin, Dapagliflozin and Metformin were found to be 2.253 min, 2.720 min, 3.276 min respectively. % RSD of the Saxagliptin, Dapagliflozin and Metformin were and found to be 0.8, 0.2 and 0.6. % Recovery was obtained as 99.60%, 100.07% and 99.95% for Saxagliptin, Dapagliflozin and Metformin respectively. LOD, LOQ values obtained from regression equations of Saxagliptin, Dapagliflozin and Metformin were 0.06, 0.12, 9.61 and 0.17, 0.36, 29.31 respectively. Regression equation of Saxagliptin is y = 40882x + 889.2, Dapagliflozin is y = 47904x + 3897 and Metformin is y = 4530.x + 35785. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.

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RP-HPLC Method Development and Validation for Simultaneous Estimation of Prazosin and Polythiazide in Bulk and Pharmaceutical Dosage Form

Cited by 5 publications

References 0 publications

Stability Indicating Method Development and Validation of Fimasartan by Reverse-Phase High-Performance Liquid Chromatography in Bulk and Pharmaceutical Dosage Form

Stability Indicating Method Development and Validation of Fimasartan by Reverse-Phase High-Performance Liquid Chromatography in Bulk and Pharmaceutical Dosage Form

Development and Validation of UV Spectrophotometric Method for Determination of Prazosin Hydrochloride

Stability Indicating and Simultaneous Determination of Saxagliptin, Dapagliflozin and Metformin in Tablet dosage form using RP-HPLC

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