RP-HPLC Method Development, Validation, and Drug Repurposing of Sofosbuvir Pharmaceutical Dosage Form: A Multidimensional Study

Mangrio, Ghulam Rasool; Maneengam, Apichit; Khalid, Zunera; Jafar, Tassadaq Hussain; Chanihoon, Ghulam Qadir; Nassani, Rayan; Unar, Ahsanullah

doi:10.1016/j.envres.2022.113282

Cited by 8 publications

(5 citation statements)

References 56 publications

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“…Further, these studies are crucial to determine the stability of the ligand-receptor complex. 94 The MD simulation results in this study confirm the firm binding of 3-octane at 200 ns. These results present pieces of evidence that 3-octane could be a promising SARS-CoV-2 M pro inhibitor, while in vitro and in vivo studies are yet required for further clinical application.…”

Section: Discussionsupporting

confidence: 76%

In silico Screening of Potential SARS-CoV-2 Main Protease Inhibitors from Thymus schimperi

Mengist

Khalid

Adane

2023

AABC

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Background COVID-19 is still instigating significant social and economic chaos worldwide; however, there is no approved antiviral drug yet. Here, we used in silico analysis to screen potential SARS-CoV-2 main protease (M pro ) inhibitors extracted from the essential oil of Thymus schimperi which could contribute to the discovery of potent anti-SARS-CoV-2 phytochemicals. Methods The absorption, distribution, metabolism, excretion, and toxicity (ADMET) profiles of compounds were determined through SwissADME and ProToxII servers. AutoDock tools were used for molecular docking analysis studies, while Chimera, DS studio, and LigPlot were used for post-docking studies. Molecular dynamic simulations were performed for 200 ns under constant pressure. Results All compounds exhibited a bioavailability score of ≥0.55 entailing that at least 55% of the drugs can be absorbed unchanged. Only five (9%), nine (16%) and two (3.6%) of the compounds showed active hepatotoxicity, carcinogenicity, and immunotoxicity, respectively. Except for flourazophore P, which showed a little mutagenicity, all other compounds did not show mutagenic properties. On the other hand, only pinene beta was found to have a little cytotoxicity. Five compounds demonstrated effective binding to the catalytic dyad of the SARS-CoV-2 M pro substrate binding pocket, while two of them (geranylisobutanoate and 3-octane) are found to be the best hits that formed hydrogen bonds with Glu 166 and Ser 144 of SARS-CoV-2 M pro . Conclusion Based on our in silico analysis, top hits from Thymus schimperi may serve as potential anti-SARS-CoV-2 compounds. Further in vitro and in vivo studies are recommended to characterize these compounds for clinical applications.

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Section: Discussionsupporting

confidence: 76%

In silico Screening of Potential SARS-CoV-2 Main Protease Inhibitors from Thymus schimperi

Mengist

Khalid

Adane

2023

AABC

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“…This finding aligns with studies conducted in various nations, which have also pinpointed low socioeconomic status and maternal education levels as salient risk factors substantially affecting childhood blood lead levels. The elevated blood lead levels observed in children from lower socioeconomic status families may be attributed to factors such as limited awareness about environmental lead sources and their consequences on children's health, coupled with a dearth of preventative measures at the individual level [24]. Moreover, financial constraints resulting from low income and the presence of numerous children in these families may render them more susceptible to lead toxicity.…”

Section: Resultsmentioning

confidence: 99%

Correlations Between Environmental Enteropathy and Anemia in Pediatric Patients Under Five Years: An Investigation of Lead Exposure

Unar,

Chanihoon

2023

Preprint

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Iron deficiency anemia and elevated blood lead levels (BPb) can have substantial health implications for young children. This study aimed to investigate the prevalence of these conditions among children aged 1 to 5 years attending a daycare center within a pediatric unit. A total of 360 children of varying genders underwent anemia screening, with 230 testing positive and 137 testing negative. Among the anemic children, the severity of anemia was classified as mild and severe based on hemoglobin levels, while non-anemic children constituted the reference group. The findings indicated that anemic children exhibited higher mean BPb values compared to non-anemic children. The prevalence of BPb levels exceeding 100 µg/L was 60% among non-anemic children, 54% among severe anemic children, and 49% among severe anemic children with BPb levels below 200 µg/L. Furthermore, a significant negative correlation was observed between BPb levels and hemoglobin and iron levels in anemic children. Additionally, there was a positive correlation between BPb levels and the age of children of both genders. In conclusion, this study highlights the significant association between BPb levels and key blood parameters, suggesting their potential as biomarkers for lead intoxication and iron-deficient anemia. Future research in this field should focus on elucidating the causal relationships and exploring intervention strategies to reduce lead exposure and prevent iron-deficient anemia in pediatric populations.

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“…These are obtained by comparing the signal to noise ratio (S/N) of blank and drug at different concentrations. The LOD value was found at 0.39 µg/ml concentration where the signal to noise ratio is found to be 3:1 and the LOQ value was found at 1.2 µg/ml with a signal to noise ratio of 10:1 [15,16].…”

Section: Limit Of Detection (Lod) and Limit Of Quantitation (Loq)mentioning

confidence: 91%

Development and validation of stability indicating RP-HPLC method for the estimation of azilsartan in Bulk

Mounika¹,

Nageswarrao²,

Kumari³

et al. 2022

ijhs

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Angiotensin-receptor blockers are often considered adequately efficacious in reducing blood pressure. By accepting the current regulatory requirements for an analytical method development, a reversed phase high performance liquid chromatographic method for routine analysis of Azilsartan has been developed. The optimized method was achieved using unisol C-18 (3μm, 4.6×150mm) coloumn with mobile phase consisting of mixture acetonitrile and methonal (90:10v/v) with a flow rate of 0.8ml/min at 249nm. The Linear concentration range is 2-10μg/ml. The detection and quantitation limit was found to be 0.45μg/ml. And the detection limit was found to be 0.149μg/ml. There are no interfering peaks under performed degradation conditions. The optimized method was validated according to ICH guidelines.

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RP-HPLC Method Development, Validation, and Drug Repurposing of Sofosbuvir Pharmaceutical Dosage Form: A Multidimensional Study

Cited by 8 publications

References 56 publications

In silico Screening of Potential SARS-CoV-2 Main Protease Inhibitors from Thymus schimperi

In silico Screening of Potential SARS-CoV-2 Main Protease Inhibitors from Thymus schimperi

Correlations Between Environmental Enteropathy and Anemia in Pediatric Patients Under Five Years: An Investigation of Lead Exposure

Development and validation of stability indicating RP-HPLC method for the estimation of azilsartan in Bulk

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