RP-HPLC method for the estimation of ceftiofur hydrochloride in bulk form

Palur, Keerthisikha; Archakam, Sreenivasa Charan; Lingasani, Nagamallika; Ranganayakulu, D.; Kumarachari, Rajasekhar Komarla; Velusamy, Shankarananth

doi:10.1016/j.jopr.2013.02.027

Cited by 8 publications

(6 citation statements)

References 8 publications

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“…The antibiotic investigated in the present work belongs to the β‐lactam family, and to the cephalosporins class . Ceftiofur hydrochloride is a third‐generation cephalosporin with a broad spectrum, possessing activity against Gram‐positive and Gram‐negative bacteria .…”

Section: Introductionmentioning

confidence: 99%

“…Ceftiofur hydrochloride is a third‐generation cephalosporin with a broad spectrum, possessing activity against Gram‐positive and Gram‐negative bacteria . The molecule has a 3‐position linkage of furoic acid thioester that differentiates it from other third generation cephalosporins . When the drug is applied, the thioester bond is cleaved to form the active metabolite desfuroylceftiofur, conjugated amino acids and disulfide dimers.…”

Section: Introductionmentioning

confidence: 99%

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Online fully automated SPE‐HPLC‐MS/MS determination of ceftiofur in bovine milk samples employing a silica‐anchored ionic liquid as sorbent

et al. 2018

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Solid-phase extraction coupled online with high performance liquid chromatography and tandem mass spectrometry was successfully applied to determine low concentrations of ceftiofur antibiotic in bovine milk samples. A silica-anchored ionic liquid was applied as sorbent material to be used as extraction phase in the proposed online system. The material was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy. In order to improve the system reproducibility, the following experimental parameters were optimized: organic solvent percentage, time and sample loading flow rate. Subsequently, the method was validated presenting satisfactory results as adequate selectivity, good linearity and correlation coefficient higher than 0.98. The limit of detection and quantification were 0.1 and 0.7 μg/L, respectively. The precision of the methodology was evaluated as repeatability and intermediate precision, with relative standard deviation values lower than 15%. The accuracy of the method ranged from 72.8 to 137% and the minimum and maximum recovery values were 73.4 and 111.3%, respectively. After the validation, seven milk samples were analyzed and although ceftiofur was not detected in any of them the method was demonstrated to be efficient when applied to the analysis of milk samples fortified with the pollutant of interest.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Online fully automated SPE‐HPLC‐MS/MS determination of ceftiofur in bovine milk samples employing a silica‐anchored ionic liquid as sorbent

et al. 2018

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“…Compared with the reported methods the proposed methods have some distinct advantages; the reported spectrophotometric methods [10][11][12][13] were less sensitive. Although the reported HPLC and LC/MS methods [3][4][5][6][7][8][17][18][19][20][21][22] are sensitive enough, they are precluded due to higher cost and non-availability of such instruments in each laboratory in developing countries. No reported spectrophotometric methods were reported for determination of TULA in its dosage form.…”

Section: Fig 1 Chemical Structure Of Ceftiofur and Tulathromycinmentioning

confidence: 99%

“…CEFT is broad-spectrum antibiotic has been used for bovine and swine respiratory disease, foot rot and metritis infections in cattle [2]. A number of HPLC [3][4][5], LC/MS [6][7][8], capillary electrophoresis [9], UV-Vis spectrophotometric [10][11][12][13] and voltametric [14,15] methods were reported for the quantification of CEFT in dosage forms and or biological samples.…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric Methods for the Determination of Ceftiofur and Tulathromycin in Bulk and Dosage Forms

Rashed

Abdelazeem

Fouad

2020

AJACR

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Two simple, accurate and precise spectrophotometric methods were developed for the determination of two veterinary drugs, ceftiofur and tulathromycin in pure form as well as in pharmaceutical formulations. The first one (Method A) based on the reducing action of ceftiofur on Fe (ΙΙΙ) to Fe (ΙΙ) in its complex with 1, 10-phenanthroline (ferrin complex) to give the orange-red colored ferroin complex that exhibits an absorption maximum at 511 nm. Ceftiofur exhibited good linearity in the concentration range of 0.3-3.0 μg mL −1 .The second method (Method B) depended on formation of a binary complex between tulathromycin and eosin Y in in the presence of carboxy methylcellulose as surfactant. Under the optimum conditions, the binary complex showed absorption maxima at 556 nm. The method obeyed Beer's law over concentration range of 1.0-15.0 μg mL −1 . The proposed methods were used for determination of the studied drugs in pharmaceutical formulation; maxfur ® powder and draxxin ® injections with mean recoveries of 99.57and 99.71%, respectively. The validity of the methods was further proved by applying the standard addition technique. A proposal of the reactions pathways were described.

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“…The chemical structure of ceftiofur hydrochloride has been illustrated in Figure 1. 2 Many gram-positive, gram negative and anaerobic bacterial pathogens of domestic animals are susceptible to ceftiofur. 3 The ready-to-use, parenteral formulation of ceftiofur hydrochloride is predominantly used against the bacterial respiratory disease affecting cattle and pigs.…”

Section: Introductionmentioning

confidence: 99%

Isocratic High Performance Liquid Chromatography Assay for Quantification of Ceftiofur Hydrochloride in Bubaline Plasma

Adil

Omer

Javeed

et al. 2019

ACSi

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We optimized and validated an isocratic high-performance liquid chromatography (HPLC) assay for quantification of ceftiofur hydrochloride in bubaline plasma. Ceftiofur, its metabolic products and protein-bound residues were cleaved, derivatized into desfuroylceftiofur acetamide and injected into HPLC system. The mobile phase comprising of sodium dihydrogen phosphate (0.025 M, pH 7) and acetonitrile (34:66, v/v), was driven at a flow rate of 1 mL/min, and separation was achieved using C18 column. Isocratic elution was performed with an injection volume of 45 µL and analyte was scanned at 310 nm. The linearity range, limit of detection and limit of quantification were 0.1-10 µg/mL, 0.03 µg/mL and 0.11 µg/mL respectively. Moreover, the accuracy, precision and recovery remained within the acceptable limits. The assay was effectively applied for determining the concentration of ceftiofur in plasma samples collected from ceftiofur-treated buffalo calves.

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RP-HPLC method for the estimation of ceftiofur hydrochloride in bulk form

Cited by 8 publications

References 8 publications

Online fully automated SPE‐HPLC‐MS/MS determination of ceftiofur in bovine milk samples employing a silica‐anchored ionic liquid as sorbent

Online fully automated SPE‐HPLC‐MS/MS determination of ceftiofur in bovine milk samples employing a silica‐anchored ionic liquid as sorbent

Spectrophotometric Methods for the Determination of Ceftiofur and Tulathromycin in Bulk and Dosage Forms

Isocratic High Performance Liquid Chromatography Assay for Quantification of Ceftiofur Hydrochloride in Bubaline Plasma

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