RP-HPLC Method for the Simultaneous Determination of Lisinopril and NSAIDs in API, Pharmaceutical Formulations and Human Serum

Abstract

High performance liquid chromatographic method was developed valdated and applied for the simultaneous determi- nation of lisinopril and NSAIDs in bulk, pharmaceuticals formulations and human serum. A Purospher star C18 (5 µm, 25 × 0.46 cm) column was used with mobile phase consisting of methanol: water: acetonitrile (80:17.5:2.5 v/v, pH 3.0) and quantitative evaluation was performed at 225 nm with a flow rate of 1.0 mL?min–1. The retention time of lisinopril was 2.2 min while naproxen, flurbiprofen, diclofenac sodium and mefenamic acid were found to be 4.0, 4.5, 5.0 and 6.7 min respectively. Suitability of this method for the quantitative determination of the drugs was proved by validation in accordance with the requirements laid down by International Conference on Harmonization (ICH) guidelines. The method is selective, precise, accurate and can be used for analysis of pharmaceutical preparations in quality control and clinical laboratories

Analytical Determination of Lisinopril Using UV Spectrophotometer and HPLC: An Overview

“…Our research group has worked on HPLC methods for the quantitation of inhibitors as captopril, enalapril and lisinopril alone and in combination with fosinopril and diclofenac sodium [12][13][14][15][16] in bulk drug, pharmaceutical formulations and serum. Sultana et al [15] have also reported simultaneous methods for the determination of various ACE inhibitors with co-administered drugs as lisinopril with H 2 antagonists [17], NSAIDs [18] and with statins [19].…”

Application of Response Surface Methodology to Estimate the Design Space of Pharmaceutical Diluents for Dispensing Powdered Formulations

“…[11][12][13][14] The HPLC system was composed of a pump (LC-10AS; Shimadzu Co., Kyoto, Japan), an auto-injector (SIL-10A, Shimadzu Co.), a UV detector (SPD-10A, Shimadzu Co.), an analysis system (Smart Chrom, KYA TECH Co., Tokyo, Japan), and a column. A C8 column (COSMOSIL 5C8-MS, 5 µm, 4.6×150 mm; Nacalai Tesque, Kyoto, Japan) was used for mexletine and a C18 column (TSKgel ODS-100V, 3 µm, 4.6×150 mm, Tosoh Co., Tokyo, Japan) was used for the other drugs.…”

Facile and Manifest Liquid Chromatographic Method for the Simultaneous Determination of Enalpril Maleate and NSAIDs in API and Pharmaceutical Formulations

“…Our research group has developed and validated simultaneous methods for the co-adminstered drugs as rosuvastatin with NSAIDs [11] and with alprazolam and diclofenac [12]. Lisinopril, captopril [13,14] and metformin [15] have also been simultaneously determined with NSAIDs.…”