RP-HPLC Separation of<i>Vaccinium myrtillus</i>Anthocyanins

Krawczyk, Urszula; Petri, Giani; Kéry, Ágnes

doi:10.1055/s-2006-960419

Cited by 2 publications

(1 citation statement)

References 2 publications

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“…Currently, RP-HPLC coupled to MS is the analytical technique commonly used to analyze polyphenolic compounds such as flavonoids in crude samples [14][15][16]. CE is a microanalytical method which is applicable for the analyses of a wide range of compounds including natural products such as flavonoids and metabolites [17][18][19][20][21]. CE provides advantages in terms of speed, it can be carried out either in the co-or counter-EOF, high efficiency, low cost and simplicity.…”

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confidence: 99%

Analysis of flavonoids by CE using capacitively coupled contactless conductivity detection

et al. 2007

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A CE method employing capacitively coupled contactless conductivity (C(4)D) compared to indirect UV-detection was developed for the analysis of phytochemically relevant flavonoids, such as 6-hydroxyflavone, biochanin A, hesperetin and naringenin. To ensure fast separation at highest selectivity, sensitivity and peak symmetry, the pH value and the concentration of the running BGE had to be optimized regarding both co- and counter-EOF mode. Optimum conditions were found to be 1.0 and 5.0 mM chromate BGE (pH 9.50) in the counter- and co-EOF mode, respectively. Validation of the established CE-C(4)D method pointed out to be approximately seven times more sensitive compared to indirect UV-detection applying the same conditions. The lower LOD defined at an S/N of 3:1 was found between 0.12 and 0.21 microg/mL for the analytes of interest using C(4)D and between 0.77 and 1.20 microg/mL using indirect UV-detection. Compared to an earlier published CE method employing direct UV-detection, C(4)D was found to be approximately two times more sensitive. Due to the lower baseline noise, C(4)D showed an excellent regression coefficient >0.99 compared to 0.93 when using indirect UV detection calibrating within a concentration range between 1 and 10 microg/mL. The influence of the sugar moiety on the conductivity of a flavonoid was studied upon the analysis of the aglycon hesperetin and the rutinosid hesperidin. The sugar moiety in hesperedin shows a higher conductivity compared to hesperetin. Finally, the optimized established CE-C(4)D method was applied to the determination and quantification of naringenin in Sinupret.

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