RP-UPLC method development and validation for the simultaneous estimation of ibuprofen and famotidine in pharmaceutical dosage form

Reddy, Yarram Ramakoti; Kumar, Kakumani Kishore; Reddy, M. Yogeshwar; Mukkanti, K.

doi:10.4103/2229-4708.103873

Cited by 26 publications

(17 citation statements)

References 14 publications

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“…Since UPLC offers numerous advantages over HPLC techniques, it has become increasingly popular and is favored over HPLC by analytical scientists (17)(18)(19)(20)(21). The simultaneous detection of drugs using UPLC techniques has been gaining importance over the past few years as it enables rapid and effective analysis as compared to traditional HPLC (22)(23)(24)(25). The UPLC method described herein can considerably simplify the analysis of PPIs.…”

Section: Discussionmentioning

confidence: 97%

Development and Validation of a UPLC Method for Rapid and Simultaneous Analysis of Proton Pump Inhibitors

et al. 2014

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Proton pump inhibitors (PPIs) are used extensively for the relief of gastroesophageal reflux, peptic ulcers, and other hypersecretory conditions. Some of the commonly used PPIs-omeprazole, esomeprazole, lansoprazole, pantoprazole, and rabeprazole-were used in this study with the aim of developing a rapid ultra performance liquid chromatography (UPLC) method for detecting each and allowing separation and quantification of a mixture of PPIs. An analysis of samples was performed on a UPLC system equipped with a quaternary solvent delivery system, a refrigerated sample manager, a column heater, a photo diode array detector scanning from 210 to 400 nm, and a C18 analytical column (50 mm × 3.0 mm, 1.7-μm particle size). The chromatographic analysis of the PPI samples and standards was performed using gradient elution with acetonitrile and water. The calibration curve range varied for each of the PPIs ranging from a lower limit of 0.75-1.78 μg/mL to a maximum concentration of 200 μg/mL with a regression coefficient (r (2)) of ≥0.98. The accuracy and precision were calculated, and the %RSD was determined to be ≤0.21% (intraday) and ≤5% (interday). The LOD was 0.23-0.59 μg/mL and the LOQ was 0.71-1.78 μg/mL for each of the drugs analyzed. The method was capable of detecting and quantifying each drug in a mixture with good resolution and a total run time of less than 5 min. Herein, we report an efficient and rapid analytical method for the simultaneous detection of multiple PPIs in a mixture.

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Section: Discussionmentioning

confidence: 97%

Development and Validation of a UPLC Method for Rapid and Simultaneous Analysis of Proton Pump Inhibitors

et al. 2014

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“…The proposed spectrofluorimetric method has the advantages of simplicity, being economic, wide availability of the instrument and less organic solvents consumption over the reported chromatographic methods (23)(24)(25)(26)(27). Moreover, the proposed method offers wider linearity ranges with better sensitivity and lower limit of detection (LOD) and limit of quantitation (LOQ) values than the reported HPTLC (28) method for the simultaneous determination of the drugs.…”

Section: Introductionmentioning

confidence: 93%

“…Several analytical procedures have been described in the literature for the analysis of this binary combination including high-pressure liquid chromatography (HPLC) (23)(24)(25)(26), ultra pure liquid chromatography (UPLC) (27), high pressure thin layer chromatography (HPTLC) (28) and spectrophotometric (23,(29)(30)(31)(32) methods. To our knowledge, no method has been reported for the spectrofluorimetric determination of such a drug combination.…”

Section: Introductionmentioning

confidence: 99%

Derivative emission spectrofluorimetry: Application to the analysis of newly approved FDA combination of ibuprofen and famotidine in tablets

Ragab

El-Kimary

2014

Luminescence

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A new combination of ibuprofen (NSAID) and famotidine (H2 receptor antagonist) was recently approved by the FDA. It was formulated to relief pain while decreasing the risk of ulceration, which is a common problem for patients receiving NSAID. A rapid and simple derivative emission spectrofluorimetric method is proposed for the simultaneous analysis of this combination in their pharmaceutical preparation. The method is based upon measurement of the native fluorescence intensity of the two drugs at λex = 233 nm in acetonitrile. The emission data were differentiated using the first (D1) derivative technique. The plots of derivative fluorescence intensity versus concentration were rectilinear over a range of 2-35 and 0.4-8 µg/mL for both ibuprofen (IBU) and famotidine (FAM), respectively. The method was sensitive as the limits of detection were 0.51 and 0.12 µg/mL and limits of quantitation were 1.70 and 0.39 µg/mL, for IBU and FAM respectively. The proposed derivative emission spectrofluorimetric method was successfully applied for the determination of the two drugs in their synthetic mixtures and tablets with good accuracy and precision. The proposed method was validated as per ICH guidelines.

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“…The literature review reveals that IBU and FAM were determined in combination by spectrophotometry [1][2][3][4][5][6][7][8][9], HPLC [6,[10][11][12][13][14][15][16][17][18][19][20], and TLC [20,21]. The aim of this work was to develop a ratio difference spectrophotometric method for determination of the cited drugs in combination without any interference from the additives or the excipients in pharmaceutical formulations.…”

Section: Introductionmentioning

confidence: 99%

Application of the ratio difference spectrophotometry to the determination of ibuprofen and famotidine in their combined dosage form; Comparison with previously published spectrophotometric methods

Zaazaa

Elzanfaly

Soudi

et al. 2015

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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RP-UPLC method development and validation for the simultaneous estimation of ibuprofen and famotidine in pharmaceutical dosage form

Cited by 26 publications

References 14 publications

Development and Validation of a UPLC Method for Rapid and Simultaneous Analysis of Proton Pump Inhibitors

Development and Validation of a UPLC Method for Rapid and Simultaneous Analysis of Proton Pump Inhibitors

Derivative emission spectrofluorimetry: Application to the analysis of newly approved FDA combination of ibuprofen and famotidine in tablets

Application of the ratio difference spectrophotometry to the determination of ibuprofen and famotidine in their combined dosage form; Comparison with previously published spectrophotometric methods

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