RP11-79H23.3 Inhibits the Proliferation and Metastasis of Non-small-cell Lung Cancer Through Promoting miR-29c

Abstract

Evidences indicate that long non-coding RNAs (lncRNAs) are closely involved and contributed to tumorigenesis and cancer progression. As a novel lncRNA, RP11-79H23.3 was found to be an anti-oncogene in bladder cancer. However, the essential roles and functions of RP11-79H23.3 in non-small-cell lung cancer (NSCLC) remains to be elucidated. Here, loss of functional assay was applied to gain insights into the functions of RP11-79H23.3 on the proliferation and metastasis capabilities of A549 and H1299 cells. Meantime, Real-time PCR was utilized to measure RP11-79H23.3 and miR-29c expression in NSCLC tissues. Dual-luciferase reporter assay, CCK8, colony formation assay, transwell and Western blot were performed to illustrate the potential molecular basis of RP11-79H23.3 in NSCLC. RP11-79H23.3 downregulation facilitated cell proliferation, migration, and invasion of NSCLC. The result of dual-luciferase reporter assay represented a direct interaction of RP11-79H23.3 with miR-29c, which suppressed miR-29c expression that showed inversely correlation in NSCLC. Moreover, RP11-79H23.3 siRNA facilitated the progression of NSCLC partially via regulating the expression of miR-29c and the activation of Wnt/β-catenin signaling pathway. Our findings highlighted that RP11-79H23.3, served as an anti-oncogene, accelerated NSCLC progression through sequestering miR-29c, providing a promising therapeutic target for NSCLC.

Exosomes in the Diagnosis of Neuropsychiatric Diseases: A Review

“…miR-335-5p targets glutamate metabotropic receptor 4 (GRM4) to regulate neuronal excitability [ 47 ], and reduced glutamate levels in specific brain regions have been implicated in the pathogenesis of depression [ 48 , 49 ]; miR-335-5p is significantly increased in the plasma exosomes of people with treatment-resistant depression [ 48 ]. miR-29c is activated by the Wnt signaling pathway [ 50 , 51 ], and in the prefrontal cortex exosomes of BD patients miR-29c is significantly increased [ 30 ].…”

Non-coding RNAs in lung cancer: molecular mechanisms and clinical applications

“…Furthermore, multiple lncRNAs and circRNAs can regulate the Wnt/ b-catenin signaling pathway by serving as competing endogenous RNA (ceRNA) for miRNAs in LC. Liu et al showed that lncRNA RP11-79H23.3 knockdown enhanced the activity of the Wnt/b-catenin signaling pathway in NSCLC cells by sequestering miR-29c, resulting in the facilitation of LC progression (98). Yang et al revealed that circ_0017109 knockdown decreased FZD4 levels by releasing miR-671-5p, resulting in the enhancement of the Wnt/b-catenin signaling pathway and subsequent facilitation of NSCLC progression (99).…”

MicroRNAs Can Influence Ovarian Cancer Progression by Dysregulating Integrin Activity

“…Several miRNAs have been reported to regulate ovarian cancer progression by directly targeting integrin mRNAs ( Table 1 ). In particular, miR-29c is reported to target integrin alpha-6 (ITGA6), inhibiting ovarian cancer cell adhesion, migration, and invasion [ 57 ]. On the contrary, Fu et al studied the expression profile of miR-93 in ovarian cancer cells resistant to cisplatin.…”