RPL1, a Gene Involved in Epigenetic Processes Regulates Phenotypic Plasticity in Rice

Zhang, Cui-Cui; Yuan, Wenya; Zhang, Qifa

doi:10.1093/mp/ssr091

Cited by 35 publications

(19 citation statements)

References 57 publications

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“…Recently, epigenetic processes have emerged as an additional level of regulation of BR responses from three independent studies. In one study, a rice mutant displaying increasing environmental-dependent phenotypic variations, RICE PLASTICITY 1 (RPL1), was found to display altered responses to BRs, gibberellin, and cytokinin, suggesting that RPL1 may regulate phenotypic plasticity through its involvement in epigenetic mechanisms affecting genes involved in plant hormone pathways (Zhang et al, 2012b). In agreement with this hypothesis, further evidence suggested that RPL1 was involved in the epigenetic modification of OsBRI1.…”

Section: Regulation Of Br Signalingmentioning

confidence: 88%

From Squalene to Brassinolide: The Steroid Metabolic and Signaling Pathways across the Plant Kingdom

Vriet¹,

Russinova²,

Reuzeau³

2013

Molecular Plant

159

132

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The plant steroid hormones, brassinosteroids (BRs), and their precursors, phytosterols, play major roles in plant growth, development, and stress tolerance. Here, we review the impressive progress made during recent years in elucidating the components of the sterol and BR metabolic and signaling pathways, and in understanding their mechanism of action in both model plants and crops, such as Arabidopsis and rice. We also discuss emerging insights into the regulations of these pathways, their interactions with other hormonal pathways and multiple environmental signals, and the putative nature of sterols as signaling molecules.

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Section: Regulation Of Br Signalingmentioning

confidence: 88%

From Squalene to Brassinolide: The Steroid Metabolic and Signaling Pathways across the Plant Kingdom

Vriet¹,

Russinova²,

Reuzeau³

2013

Molecular Plant

159

132

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“…3B). At the same time, we used the rice nuclear protein RPL1 fused with cyan fluorescent protein (CFP) as a nuclear marker (Zhang et al, 2012) and cotransformed it into rice protoplasts with 35S:OsHTAS-GFP. The cyan fluorescence overlapped with the green fluorescence (Fig.…”

Section: Subcellular Localization Of Oshtasmentioning

confidence: 99%

“…Primers for other target genes were designed using the Roche Web site (Roche Applied Science). The rice ACTIN1 gene was used as the internal reference (Zhang et al, 2012), and data analyses with the 2 -DDCt method were performed as described (Livak and Schmittgen, 2001).…”

Section: Rna Extraction and Qrt-pcr Analysismentioning

confidence: 99%

The RING Finger Ubiquitin E3 Ligase OsHTAS Enhances Heat Tolerance by Promoting H2O2-Induced Stomatal Closure in Rice

et al. 2015

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Heat stress often results in the generation of reactive oxygen species, such as hydrogen peroxide, which plays a vital role as a secondary messenger in the process of abscisic acid (ABA)-mediated stomatal closure. Here, we characterized the rice (Oryza sativa) HEAT TOLERANCE AT SEEDLING STAGE (OsHTAS) gene, which plays a positive role in heat tolerance at the seedling stage. OsHTAS encodes a ubiquitin ligase localized to the nucleus and cytoplasm. OsHTAS expression was detected in all tissues surveyed and peaked in leaf blade, in which the expression was concentrated in mesophyll cells. OsHTAS was responsive to multiple stresses and was strongly induced by exogenous ABA. In yeast two-hybrid assays, OsHTAS interacted with components of the ubiquitin/26S proteasome system and an isoform of rice ascorbate peroxidase. OsHTAS modulated hydrogen peroxide accumulation in shoots, altered the stomatal aperture status of rice leaves, and promoted ABA biosynthesis. The results suggested that the RING finger ubiquitin E3 ligase OsHTAS functions in leaf blade to enhance heat tolerance through modulation of hydrogen peroxide-induced stomatal closure and is involved in both ABA-dependent and DROUGHT AND SALT TOLERANCE-mediated pathways.

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“…First-strand cDNA was generated using the Perfect Real Time Primescript RT reagent (TaKaRa, Japan) in 20 μL of reaction mixture containing 5 μg of total RNA following the manufacturer’s instructions. qRT-PCR was performed in triplicate on a Roche LightCycler ® 96 system as described (Zhang et al 2012). Data analyses with the 2 −DDCT method were performed as previously described (Livak and Schmittgen 2001).…”

Section: Methodsmentioning

confidence: 99%

Pyramiding and evaluation of both a foreign Bacillus thuringiensis and a Lysine-rich protein gene in the elite indica rice 9311

Liu

Zhang

et al. 2016

Breed. Sci.

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Gene pyramiding is an efficient approach for the genetic improvement of multiple agronomic traits simultaneously. In this study, we pyramided two foreign genes, cry1Ac driven by the rice Actin I promoter, and lysine-rich protein (LRP), driven by the endosperm-specific GLUTELIN1 (GT1) promoter, into the elite indica cultivar 9311. These two genes were chosen in an attempt to enhance insect-resistance and Lysine (Lys) content. In the pyramided line, the foreign gene cry1Ac was efficiently expressed in the leaves and stems, and exhibited highly efficient resistance to striped stem borer (SSB, Chilo suppressalis Walker) in the laboratory and rice leaf folder (RLF, Cnaphalocrocis medinalis Guenee) in the field. Furthermore, the LRP gene was highly expressed in the endosperm and produced a remarkable increase of Lys content in the seeds of the pyramided line. The data from field trials demonstrated that most of the agronomic traits including yield were well maintained in the pyramided line compared to the parental control. These results strongly suggest that the foreign cry1Ac and LRP genes have remarkable application potential in rice, and the resultant pyramided line serves as an ideal bridge material for the improvement of insect-resistance and high Lys rice in the future.

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RPL1, a Gene Involved in Epigenetic Processes Regulates Phenotypic Plasticity in Rice

Cited by 35 publications

References 57 publications

From Squalene to Brassinolide: The Steroid Metabolic and Signaling Pathways across the Plant Kingdom

From Squalene to Brassinolide: The Steroid Metabolic and Signaling Pathways across the Plant Kingdom

The RING Finger Ubiquitin E3 Ligase OsHTAS Enhances Heat Tolerance by Promoting H2O2-Induced Stomatal Closure in Rice

Pyramiding and evaluation of both a foreign <i>Bacillus thuringiensis</i> and a <i>Lysine-rich protein</i> gene in the elite <i>indica</i> rice 9311

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