RPMI 2650 epithelial model and three-dimensional reconstructed human nasal mucosa as in vitro models for nasal permeation studies

Retinoic acid and hydrocortisone strengthen the barrier function of human RPMI 2650 cells, a model for nasal epithelial permeability

“…The cell index of the RPMI 2650 layers was significantly increased following a 24-h retinoic acid treatment at 300 lg/mL concentration; although the other lower doses (0.01 and 100 lg/mL) had no effect. We could confirm the absence of efficacy at the lowest dose on this cell line (Wengst and Reichel 2010). The effect of retinoic acid was independent from the presence of serum and it elevated the cell index compared to the control groups at all serum concentrations tested (Fig.…”

“…Well-differentiated mucociliary phenotype of human nasal epithelium cultures was also reported without retinoids (Van Scott et al 1988;Werner and Kissel 1995;Agu et al 2001). Retinoic acid at 0.01 lg/mL concentration had no effect on a nasal model based on RPMI 2650 cells (Wengst and Reichel 2010).…”

“…Furthermore, there are inconclusive data on the ability of RPMI 2650 cells to form tight junctions, though there is evidence that they can form perijunctional F-actin rings (Werner and Kissel 1996). Due to these reasons RPMI 2650 human nasal epithelial cell line is rarely used for nasal transport studies (Bai et al 2008;Wengst and Reichel 2010).…”

Innovative pharmaceutical formulations and excipients for nasal delivery of drugs to target the systemic circulation

“…In another study RPMI 2650 cells grew in multilayers, typical for the nasal epithelium, but cell polarisation and differentiation could not be detected . In contrast to the above mentioned studies, using different culture conditions including air-liquid interface RPMI 2650 cells formed layers suitable for permeability assays (Bai et al, 2008;Wengst and Reichl, 2010).…”

Application of wet milling techniques to produce micronized and nanonized drug pre-dispersions for the development of intranasal formulations

“…Although there are drawbacks of the ex vivo tissue and in vivo animal models, including differences between the species, specialities in many anatomical and physiological features in various animal nasal cavities (Chien et al, 2008). A cell culture model based on human RPMI 2650 cell line has been mostly used for nasal metabolism studies and toxicity assays but it can provide information on nasal permeability as well (Bai et al, 2008;Wengst and Reichl, 2010;.…”