RPS24 knockdown inhibits colorectal cancer cell migration and proliferation in vitro

Wang, Yue; Sui, Jinke; Xu, Li; Cao, Fuao; He, Jian; Yang, Bo; Zhu, Xiaoming; Sun, Yanan; Pu, Yong

doi:10.1016/j.gene.2015.06.084

Cited by 37 publications

(30 citation statements)

References 20 publications

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“…Consistent with these findings, elevated expression of phosphorylated S6 is associated with metastatic tumors and with shorter metastasis-free survival in patients with lung adenocarcinomas (McDonald et al, 2008). Moreover, S3, S15A, and S24 were identified to positively regulate migration of osteosarcoma cells, glioma cells, and colorectal cancer cells in vitro, respectively (Nagao- Kitamoto et al, 2015;Wang et al, 2015b;Yao et al, 2015). Conversely, L15 and S7 were reported to negatively regulate cell invasion and migration in vitro and in vivo through repressing epithelial-mesenchymal transition (EMT) Yan et al, 2015).…”

Section: Cell Migration and Invasionmentioning

confidence: 64%

The role of ribosomal proteins in the regulation of cell proliferation, tumorigenesis, and genomic integrity

Xiong

Sun

2016

Sci. China Life Sci.

109

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Ribosomal proteins (RPs), the essential components of the ribosome, are a family of RNA-binding proteins, which play prime roles in ribosome biogenesis and protein translation. Recent studies revealed that RPs have additional extra-ribosomal functions, independent of protein biosynthesis, in regulation of diverse cellular processes. Here, we review recent advances in our understanding of how RPs regulate apoptosis, cell cycle arrest, cell proliferation, neoplastic transformation, cell migration and invasion, and tumorigenesis through both MDM2/p53-dependent and p53-independent mechanisms. We also discuss the roles of RPs in the maintenance of genome integrity via modulating DNA damage response and repair. We further discuss mutations or deletions at the somatic or germline levels of some RPs in human cancers as well as in patients of Diamond-Blackfan anemia and 5q-syndrome with high susceptibility to cancer development. Moreover, we discuss the potential clinical application, based upon abnormal levels of RPs, in biomarker development for early diagnosis and/or prognosis of certain human cancers. Finally, we discuss the pressing issues in the field as future perspectives for better understanding the roles of RPs in human cancers to eventually benefit human health. ribosomal protein, tumorigenesis, genomic integrity, ribosomal stress, p53, MDM2 Citation:Xu, X., Xiong, X., and Sun, Y. (2016). The role of ribosomal proteins in the regulation of cell proliferation, tumorigenesis, and genomic integrity. Sci China Life Sci 59, 656 -672.

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Section: Cell Migration and Invasionmentioning

confidence: 64%

The role of ribosomal proteins in the regulation of cell proliferation, tumorigenesis, and genomic integrity

Xiong

Sun

2016

Sci. China Life Sci.

109

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“…These results may explain why OA often occurs in older females. RPS24 insufficiency inhibits the proliferation of cancer cells ( 26 ) and causes distinct cell cycle defects in diamond-blackfan anemia ( 27 ). The biological functions of RPS28, RPS18 and RPS4X in OA remain elusive at present.…”

Section: Discussionmentioning

confidence: 99%

Identification of genes and pathways in the synovia of women with osteoarthritis by bioinformatics analysis

Liu

Zhou

et al. 2018

Mol Med Report

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Osteoarthritis (OA) has a high prevalence in female patients and sex may be a key factor affecting the progression of OA. The aim of the present study was to identify genetic signatures in the synovial membranes of female patients with OA and to elucidate the potential associated molecular mechanisms. The gene expression profiles of the GSE55457 and GSE55584 datasets were obtained from the Gene Expression Omnibus database. Data of two synovial membranes from normal female individuals (GSM1337306 and GSM1337310) and two synovial membranes from female patients affected by OA (GSM1337327 and GSM1337330) were obtained from the dataset GSE55457, and those of three synovial membranes from female patients affected by OA (GSM1339628, GSM1339629 and GSM1339632) were obtained from the dataset GSE55584. Differentially expressed genes (DEGs) were identified by using Morpheus software. Protein-protein interaction (PPI) networks of the DEGs were constructed by using Cytoscape software. Subsequently, Gene Ontology (GO) function and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment analyses of the top module of the PPI network were performed by using ClueGo. A total of 377 DEGs were identified in the synovial membranes of OA patients compared with those of normal individuals, including 164 upregulated and 213 downregulated genes. The top 10 hub genes were ubiquitin (UB)C, ribosomal protein (RP) L23A, mammalian target of rapamycin, heat shock protein 90 α family class A member 1, RPS28, RPL37A, RPS24, RPS4X, RPS18 and UBB. The results of the GO analysis indicated that the DEGs included in the top module of the PPI were mainly enriched in the terms ‘nuclear-transcribed mRNA catabolic process’, ‘nonsense mediated decay’, and ‘cytoplasmic translation and ribosomal small subunit biogenesis’. KEGG pathway analysis indicated that the DEGs included in the top one module were mainly enriched in the ‘ribosome’ pathway. The present study provides a systematic, molecular-level understanding of the degeneration of the synovial membrane in the progression of OA in female patients. The hub genes and molecules associated with the synovial membrane may be used as biomarkers and therapeutic targets for the treatment of OA in female patients with OA.

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“…The efficacy of RPS24 as a target for diagnosis or treatment of colon carcinoma has previously been evaluated following the discovery that rps24 knockdown in CRC cell lines (HCT116 and HT-29) reduced cell proliferation and hindered cell migration rate [ 29 ]. The study went on to conclude that the rps24 gene may have a critical role in colon cancer.…”

Section: Discussionmentioning

confidence: 99%

Expression of ribosomal proteins in normal and cancerous human prostate tissue

et al. 2017

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Few quantifiable tissue biomarkers for the diagnosis and prognosis of prostate cancer exist. Using an unbiased, quantitative approach, this study evaluates the potential of three proteins of the 40S ribosomal protein complex as putative biomarkers of malignancy in prostate cancer. Prostate tissue arrays, constructed from 82 patient samples (245 tissue cores, stage pT3a or pT3b), were stained for antibodies against three ribosomal proteins, RPS19, RPS21 and RPS24. Semi-automated Ox-DAB signal quantification using ImageJ software revealed a significant change in expression of RPS19, RPS21 and RPS24 in malignant vs non-malignant tissue (p<0.0001). Receiver operating characteristics curves were calculated to evaluate the potential of each protein as a biomarker of malignancy in prostate cancer. Positive likelihood ratios for RPS19, RPS21 and RPS24 were calculated as 2.99, 4.21, and 2.56 respectively, indicating that the overexpression of the protein is correlated with the presence of disease. Triple-labelled, quantitative, immunofluorescence (with RPS19, RPS21 and RPS24) showed significant changes (p<0.01) in the global intersection coefficient, a measure of how often two fluorophore signals intersect, for RPS19 and RPS24 only. No change was observed in the co-localization of any other permutations of the three proteins. Our results show that RPS19, RPS21 or RPS24 are upregulated in malignant tissue and may serve as putative biomarkers for prostate cancer.

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RPS24 knockdown inhibits colorectal cancer cell migration and proliferation in vitro

Cited by 37 publications

References 20 publications

The role of ribosomal proteins in the regulation of cell proliferation, tumorigenesis, and genomic integrity

The role of ribosomal proteins in the regulation of cell proliferation, tumorigenesis, and genomic integrity

Identification of genes and pathways in the synovia of women with osteoarthritis by bioinformatics analysis

Expression of ribosomal proteins in normal and cancerous human prostate tissue

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