RR‐1 cuticular protein <i>TcCPR69</i> is required for growth and metamorphosis in <i>Tribolium castaneum</i>

Xie, Jia; Peng, Guifang; Wang, Miao; Zhong, Qisheng; Song, Xiaodong; Bi, Jian; Tang, Jing; Feng, Fan; Gao, Han; Li, Bin

doi:10.1111/1744-7917.13038

Cited by 21 publications

(11 citation statements)

References 60 publications

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“…RR-1 gene LmAbd-9 highly expressed after every ecdysis, functioning in postecdysis endocuticle synthesis in Locusta migratoria (Zhao et al 2019). TcCPR69 is required for growth and metamorphosis, and TcCPR4 is necessary for the formation of pore canals in Tribolium castaneum (Noh et al 2015, Xie et al 2022). The deletion of BmorCPR2 altered the overall morphology of larvae and the normal larval body shape in silkworm (Qiao et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Expressions and functions of RR-1 cuticular protein genes in the integument of Mythimna separata

Xing

et al. 2023

Journal of Economic Entomology

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As the most outer layer between itself and the environment, integuments are necessary for insects with various important functions. Cuticular proteins (CPs) are the main components in integuments, while the functions of CP genes remain unknown in Mythimna separata (Walker), which is a devastating agricultural pest. In this study, 79 CP genes were identified from the transcriptomes of larval integuments, 57 of which were from the family containing conserved Rebers & Riddiford (R&R) consensus (CPR family). Amongst these CPRs, 44 genes belonged to the subfamily with RR-1 motif (RR-1 genes) and clustered into three clades, with the top 15 most abundant RR-1 genes identified based on fragments per kilobase per million mapped fragments (FPKM) values. RT-qPCR analysis showed that most of RR-1 genes such as MsCPR1-4 were highly expressed at larval stages and in their integuments. The expression levels of RR-1 genes were generally decreased at the beginning but increased at the late stage of molting process. RNAi was applied for six RR-1 genes, and MsCPR1-4 were knocked down significantly. Silence of MsCPR2 resulted in abnormal integument formed after molting, while knockdown of MsCPR3 and MsCPR4 led to failure of molting, respectively. No phenotype was obtained for the RNAi of MsCPR1. Therefore, the expression of RR-1 genes and their functions were analyzed in the development of integuments in M. separata, providing new insights of RR-1 genes and potential targets for the development of growth regulators and new insecticides for M. separata.

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Section: Discussionmentioning

confidence: 99%

Expressions and functions of RR-1 cuticular protein genes in the integument of Mythimna separata

Xing

et al. 2023

Journal of Economic Entomology

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“…Insect body materials were cut into about 70 nm ultrathin sections by using a Leica CM1850 cryotome (Leica Microsystems, Germany). The cuticle chitin and cell nucleus of ultrathin sections were stained using 50 μg/mL WGA-FITC and 10 μg/mL DAPI for 15–20 min, separately, and then washed three times for 12–15 min per time using sterile PBS buffer as previously described. , A Leica SP8 confocal microscope was used to capture the fluorescence images.…”

Section: Methodsmentioning

confidence: 99%

miR-2765 Modulates the Seasonal Polyphenism in Cacopsylla chinensis by Targeting a Novel Cold Rreceptor CcTRPC3

Li,

Zhang,

Zhang

et al. 2023

J. Agric. Food Chem.

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Polyphenism is a beneficial way in organisms to better cope with changing circumstances and is a hot topic in entomology, evolutionary biology, and ecology. Until now, this phenomenon has been proven to be season-, density-, and dietdependent; however, there are very few reports on temperature regulation. Cacopsylla chinensis showed seasonal polyphenism, namely as summer-and winter-form, with obvious diversity in phenotypic characteristics in response to seasonal variation. Previous studies have found that low temperature in autumn is an extremely important element in inducing summer-form change to winterform, but the underlying regulatory mechanism is still a mystery. Herein, we provided the initial evidence that the third instar of the summer-form is the critical period for developing to the winter-form, and 10 °C induces this transition by affecting the total pigment, chitin level, and thickness of the cuticle. Second, CcTPRC3 was proven to function as a novel cold receptor to control this seasonal polyphenism. Moreover, miR-2765 was found to mediate seasonal polyphenism by inhibiting CcTRPC3 expression. Last, we found that cuticle binding proteins CcCPR4 and CcCPR9 function as the downstream signals of CcTRPC3 to regulate the seasonal polyphenism in C. chinensis. In conclusion, our results displayed a novel signal pathway of miR-2765 and CcTRPC3 for the regulation of seasonal polyphenism in C. chinensis. These findings provide insights into the comprehensive analysis of insect polyphenism and are useful in developing potential strategies to block the phase transition for the pest control of C. chinensis.

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“…In insects, the cuticle has dissimilar properties at different developmental stages and in different body parts. During early developmental stages, the cuticle becomes flexible and elastic, and becomes sclerotized and melanized shortly after molting [ 13 , 14 , 15 , 16 ]. This process occurs periodically during the growth and development of insects.…”

Section: Introductionmentioning

confidence: 99%

“…This process occurs periodically during the growth and development of insects. The hard exoskeleton acts as a protective barrier, while the flexible cuticle allows the insects to move [ 13 , 17 ]. Ae.…”

Section: Introductionmentioning

confidence: 99%

Elongases of Long-Chain Fatty Acids ELO2 and ELO9 Are Involved in Cuticle Formation and Function in Fecundity in the Yellow Fever Mosquito, Aedes aegypti

Jing

Chen

et al. 2023

Insects

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Long-chain fatty acid elongases (ELOs) play important roles in the metabolism of fatty acids in insects. In this study, the genes for two elongases from Aedes aegypti were identified, AeELO2 and AeELO9. Quantitative real time PCR showed that AeELO2 and AeELO9 are expressed at all developmental stages and some body parts, but with different expression patterns. RNAi-mediated knockdown of AeELO2 and AeELO9 was performed to investigate their roles in the development, growth, osmotic balance, and cold tolerance of Ae. aegypti. Knockdown of AeELO2 slowed larval growth and development by causing molting abnormalities. Additionally, 33% ± 3.3% of adults died during oviposition, accompanied by an abnormal extension of cuticles in AeELO2-dsRNA knockdown mosquitos. Knockdown of AeEL09 resulted in abnormal balance of cuticular osmotic pressure and a reduction in egg production. The maximal mRNAs of AeELO2 and AeELO9 were detected in eggs at 72 h after oviposition. Moreover, AeELO2 knockdown reduced the egg hatching rates and AeELO9 knockdown larvae did not develop well. In summary, AeELO2 is involved in larval molting and growth, and its knockdown affects the flexibility and elasticity of adult mosquito cuticles. AeELO9 regulates cold tolerance, osmotic balance, and egg development in Ae. aegypti.

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RR‐1 cuticular protein TcCPR69 is required for growth and metamorphosis in Tribolium castaneum

Cited by 21 publications

References 60 publications

Expressions and functions of RR-1 cuticular protein genes in the integument of Mythimna separata

Expressions and functions of RR-1 cuticular protein genes in the integument of Mythimna separata

miR-2765 Modulates the Seasonal Polyphenism in Cacopsylla chinensis by Targeting a Novel Cold Rreceptor CcTRPC3

Elongases of Long-Chain Fatty Acids ELO2 and ELO9 Are Involved in Cuticle Formation and Function in Fecundity in the Yellow Fever Mosquito, Aedes aegypti

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