RsaOG, a new Staphylococcal family of highly transcribed non-coding RNA

Abstract

T he expression of trans-acting smallRNAs in firmicutes has been poorly documented to date. This gap is being filled quickly in the genus Staphylococcus, which is both a model firmicute and an important human pathogen. Here we analyse RsaOG, a novel small RNA family specific to Staphylococcus and highly transcribed. This well conserved element, first discovered in a computational screen, was precisely mapped in the genome by RACE mapping and the identification of a putative transcriptional promoter. The proposed secondary structure presents two highly conserved unpaired sequences, part of which can form a pseudoknot. We suggest a possible involvement of the remaining conserved single stranded region in trans regulatory interactions.

sRNA and cis-antisense sRNA identification in Staphylococcus aureus highlights an unusual sRNA gene cluster with one encoding a secreted peptide

“…Most of these studies were done using methicillin-resistant strain N315 11, 18 . In others, newly discovered sRNAs were described based on their genomic location in that particular strain 9, 12, 13, 16 . S. aureus sRNA identification has been hampered by the lack of naming consensus and the absence of dedicated annotation files.…”

A multifaceted small RNA modulates gene expression upon glucose limitation in Staphylococcus aureus

“…In this work, we have investigated the cellular functions of one abundant sRNA, called RsaI (or RsaOG), which is highly conserved among Staphylococcaea (Geissmann et al, 2009;Marchais et al, 2010). In contrast to many sRNAs that contained C-track motifs, this RNA has the particularity to carry two conserved G-rich sequences and a large unpaired CU-rich interhelical region (Fig 2).…”

“…This 144 nucleotides-long sRNA is highly conserved among Staphylococcacea and carries two conserved regions including two G-track sequences and a long unpaired interhelical region rich in pyrimidines (Geissmann et al, 2009;Marchais et al, 2010). This 144 nucleotides-long sRNA is highly conserved among Staphylococcacea and carries two conserved regions including two G-track sequences and a long unpaired interhelical region rich in pyrimidines (Geissmann et al, 2009;Marchais et al, 2010).…”

“…Here, we have identified a signaling pathway responding to glucose uptake, which involves a sRNA, called RsaI (or RsaOG). This 144 nucleotides-long sRNA is highly conserved among Staphylococcacea and carries two conserved regions including two G-track sequences and a long unpaired interhelical region rich in pyrimidines (Geissmann et al, 2009;Marchais et al, 2010). The expression of RsaI is observed exclusively at the stationary phase of growth in rich medium (Geissmann et al, 2009) and is enhanced after vancomycin exposure (Howden et al, 2013).…”

A dual sRNA inStaphylococcus aureusinduces a metabolic switch responding to glucose consumption

“…For instance, E. 518 coli and Salmonella SgrS contributed to stress resistance and growth during glucose-519 phosphate stress (reviewed in Bobrovskyy and Vanderpool, 2014), Salmonella GcvB 520 regulon is required for growth if peptides represent the unique carbon source (Miyakoshi et 521 al., 2015), while E. coli Spot42 is important for optimal utilization of carbon sources and its 522 overproduction inhibited growth, when succinate was the sole carbon source (Møller et al, 523 2002). In S. aureus, the overproduction of RsaE induced a growth defect, which was partially 524 alleviated by acetate (Bohn et al, 2010). Therefore, the yields of these sRNAs should be 525 tightly controlled during growth in order to adjust the metabolic pathways, to optimize the use 526 of the preferred carbon sources, and to avoid cell damages or metabolites depletion caused 527 by the intracellular production of glucose-phosphate.…”