RT-PCR based analysis of cell culture negative stools samples from poliomyelitis suspected cases

Santos, Ana Paula dos; Costa, Eliane Veiga da; Oliveira, Silas S.; Souza, Michele C; Silva, Edson E. da

doi:10.1016/s1386-6532(01)00211-6

Cited by 17 publications

(10 citation statements)

References 11 publications

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“…For instance, only 12.8% of all of the AFP cases reported in this study were positively diagnosed for enteroviruses by virus isolation. The low sensitivity of cell culture may be due to a low concentration of infectious viruses in stool or the presence of enterovirus serotypes (especially coxsackievirus group A) that grow poorly or not at all in tissue culture (5,11). Cell-culture sensitivity may be affected by toxicity of the samples and inappropriate transport or storage of live organisms, which does favor less viral infectivity but not necessarily detection by RT-PCR (12).…”

Section: Discussionmentioning

confidence: 99%

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Comparison of cell culture with RT‐PCR for enterovirus detection in stool specimens from patients with acute flaccid paralysis

Shoja

Tabatabie

Shahmahmoudi

et al. 2007

Clinical Laboratory Analysis

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Since October 2000, Iran has been declared polio-free by the World Health Organization (WHO). Despite the fact that poliomyelitis caused by polioviruses has been eliminated from Iran, the number of acute flaccid paralysis (AFP) cases has not been reduced. Therefore, it is of great importance to investigate the other viral agents that may cause AFP (mainly nonpolio enteroviruses, which play a significant role in the etiology of neurological syndromes). Some enteroviruses do not grow in the conventional cell lines that are being used for enterovirus detection. Furthermore, the virus titer is an important factor in the sensitivity of cell culture to detect the virus. The fact that cell culture is a time-consuming procedure is another reason to find a more practical method for enterovirus detection. Therefore, a more sensitive and rapid method should be used to detect enteroviruses as efficiently as possible in the stool specimens of AFP cases. The aim of this study was to evaluate cell culture and RT-PCR in enterovirus detection. Findings have shown that RT-PCR can increase the rate of nonpolio enterovirus detection by up to 10% in comparison with cell culture. Also, the rapid detection of enteroviruses by RT-PCR can decrease both the unnecessary use of antibiotics and the costs in clinical practice. For this reason, we find that RT-PCR is a more practical technique for enterovirus detection.

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Section: Discussionmentioning

confidence: 99%

“…Some enteroviruses do not grow in the conventional cell lines that are being used for enterovirus detection (5). Furthermore, the virus titer is an important factor in the sensitivity of cell culture to detect the virus.…”

Section: Introductionmentioning

confidence: 99%

Comparison of cell culture with RT‐PCR for enterovirus detection in stool specimens from patients with acute flaccid paralysis

Shoja

Tabatabie

Shahmahmoudi

et al. 2007

Clinical Laboratory Analysis

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“…Surveillance for AFP is recommended by the World Health Organization (WHO) using the standard approach for poliovirus surveillance for the purposes of Global Polio Eradication (Anonymous, 2006). A wide variety of nonpolio enteroviruses and other picornaviruses have been identified in stool specimens of AFP cases (Bingjun et al, 2008;Kapoor et al, 2008;Oberste et al, 2006;Saeed et al, 2007;Santos et al, 2002;Shoja et al, 2007). Detection of high-prevalence agents from a non-sterile site is not sufficient to conclusively establish an aetiological link of picornavirus infection with disease; however, given the known ability of many picornaviruses to cause central nervous system (CNS) disease, it is likely that these viruses contribute to at least a fraction of AFP cases.…”

Section: Introductionmentioning

confidence: 99%

Diversity of picornaviruses in rural Bolivia

Nix

Khetsuriani

Peñaranda

et al. 2013

Journal of General Virology

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The family Picornaviridae is a large and diverse group of viruses that infect humans and animals. Picornaviruses are among the most common infections of humans and cause a wide spectrum of acute human disease. This study began as an investigation of acute flaccid paralysis (AFP) in a small area of eastern Bolivia, where surveillance had identified a persistently high AFP rate in children. Stools were collected and diagnostic studies ruled out poliovirus. We tested stool specimens from 51 AFP cases and 34 healthy household or community contacts collected during 2002–2003 using real-time and semi-nested reverse transcription polymerase chain reaction assays for enterovirus, parechovirus, cardiovirus, kobuvirus, salivirus and cosavirus. Anecdotal reports suggested a temporal association with neurological disease in domestic pigs, so six porcine stools were also collected and tested with the same set of assays, with the addition of an assay for porcine teschovirus. A total of 126 picornaviruses were detected in 73 of 85 human individuals, consisting of 53 different picornavirus types encompassing five genera (all except Kobuvirus). All six porcine stools contained porcine and/or human picornaviruses. No single virus, or combination of viruses, specifically correlated with AFP; however, the study revealed a surprising complexity of enteric picornaviruses in a single community.

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“…This method, known as PCR, has allowed the detection of hard to culture pathogens for traditional microbiological laboratories [13][14][15][16][17]. Even though this technique just exposes the presence of genetic material of the procured microorganism (this not implying evidence of the presence of a viable organism), PCR has been demonstrated to be quite useful when is together with an appropriate clinical approach.…”

Section: Discussionmentioning

confidence: 99%

Using Real Time PCR For The Etiological Diagnosis Of Viral Encephalitis

Rubinstein¹

2013

J Neurol Disord

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RT-PCR based analysis of cell culture negative stools samples from poliomyelitis suspected cases

Cited by 17 publications

References 11 publications

Comparison of cell culture with RT‐PCR for enterovirus detection in stool specimens from patients with acute flaccid paralysis

Comparison of cell culture with RT‐PCR for enterovirus detection in stool specimens from patients with acute flaccid paralysis

Diversity of picornaviruses in rural Bolivia

Using Real Time PCR For The Etiological Diagnosis Of Viral Encephalitis

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