RT-PCR based detection of Pepper mild mottle virus from capsicum seeds and seed transmission assay

Patel, Priyankaben; Kumari, Nidhi

doi:10.1007/s13337-023-00807-0

Cited by 3 publications

(6 citation statements)

References 23 publications

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“…In contrast to the high rate of seed transmission observed by other researchers, an average seed transmission rate of 10%-30% was recorded for the 'California Wonder', 'Marconi', 'Yolo Wonder', and 'Anaheim' pepper varieties (59). In our experiment, average seed transmission rates of 55.56%, 40.64%, and 28.96% were recorded for 'California Wonder', 'Doux des Landes', and 'Yolo Wonder', respectively, using a direct RT-PCR assay of their seeds (56). The rate of seed transmission decreased with an increase in seed storage duration at room temperature (40, 58, 60).…”

Section: Transmissionmentioning

confidence: 48%

“…This serves as a primary infection source. A seed tobamoviruses transmission rate of between 0% and 65.3% was reported for the Capsicum species (10, 40, 54,56,58). In contrast to the high rate of seed transmission observed by other researchers, an average seed transmission rate of 10%-30% was recorded for the 'California Wonder', 'Marconi', 'Yolo Wonder', and 'Anaheim' pepper varieties (59).…”

Section: Transmissionmentioning

confidence: 79%

“…Since PMMoV can be transmitted through contaminated seeds and virus-infected soils, it has the potential to spread during the planting process. Many studies have reported on the seed-borne nature of PMMoV (10, [54][55][56]. PMMoV resides on the outer coat of seeds but is very rarely found in the embryo and endosperm, which means that its transmission occurs nonembryonically (57).…”

Section: Transmissionmentioning

confidence: 99%

“…The rate of seed transmission decreased with an increase in seed storage duration at room temperature (40, 58, 60). Even a very low proportion of infected seeds can cause an epidemic in a field or polyhouse (56). PMMoV absorbed by the soil, plant debris (leaves, stems, and roots), greenhouse structures, humus, and working tools can remain stable for extended periods, serving as an inoculum (12,61).…”

Section: Transmissionmentioning

confidence: 99%

“…Electron microscopy is the preeminent technique employed by virologists to ascertain the association of tobamoviruses with corresponding plant samples by means of visualizing rod-shaped rigid virion particles (11,24,87). DAS-ELISA, a widely employed serological assay for high-throughput screening, is frequently utilized to detect PMMoV infection in plant tissues, including seeds, and in soil (14,38,43,56,61,79,88). In addition to DAS-ELISA, the utilization of non-precoated indirect ELISA (Id-ELISA) has also proven valuable for the detection of PMMoV in infected soil (61,89).…”

Section: Detection and Diagnosis Of Pmmovmentioning

confidence: 99%

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Pepper mild mottle virus: a formidable foe of capsicum production—a review

Kumari,

Sharma,

Patel

et al. 2023

Front. Virol.

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Viruses are one of the major restraining factors in pepper cultivation globally. Among different viruses, pepper mild mottle virus (PMMoV) is one of the most detrimental plant viruses infecting Capsicum spp. belonging to the genus Tobamovirus and Virgaviridae family. It has a monopartite positive-sense single-stranded RNA genome of 6.35 kb size. On an average, PMMoV results in 15%–40% losses in capsicum fruit yield. However, the incidence of PMMoV can reach as high as 95%, leading to substantial yield losses ranging from 75% to 95%. The virus is transmitted via contact, soil, and seeds rather than via insect vectors. PMMoV, because of its seed-borne nature, now occurs worldwide. PMMoV mainly infects Capsicum spp. under natural conditions; however, it can experimentally be transmitted to other plants species belonging to the families Solanaceae, Cucurbitaceae, Labiatae, Chenopodiaceae, and Plantaginaceae. The resistance to tobamoviruses in capsicum is conferred by L locus. Mutations in the coat protein of PMMoV are responsible for the emergence of L-mediated resistance-breaking pathotypes. The highly contagious nature of the virus, seed transmission behavior, and the emergence of virulence complicate its management through a single approach. Therefore, efforts are directed towards providing a more practical and efficient integrated management solution using the RNA interference approach; exploitation of the L gene for resistance breeding; and the inhibitory potential of natural products, systemic resistance-inducing antagonistic bacteria, and chemically synthesized silver nanoparticles. Markers linked to L alleles have been observed to accelerate capsicum breeding programs through marker-assisted selection. In this study, an attempt has been made to compile the recent developments in PMMoV biology, pathogenic variability, genomic organization, and management strategies.

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Section: Transmissionmentioning

confidence: 48%