RT- PCR testing of upper respiratory tract samples for diagnosis of SARS-CoV-2: Between justification and overestimation, a multi-center international study

Hammad, Noha M.; Saeed, Maysaa Abdallah; Shaltout, Shaker Wagih; Nofal, Hanaa A.; Nafae, Ramadan M.; Arslan, Kadem; Tanoğlu, Alpaslan; Nechifor, M; Luca, Cătălina; Al-kadhim, Zaid Hashim Ali; Mosallem, Ahmed; Amer, Fatma

doi:10.1016/j.tmaid.2022.102334

Cited by 3 publications

(4 citation statements)

References 46 publications

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“…Based on the features seen in unenhanced chest CT scans, the COVID-19 Reporting and Data System (CO-RADS) is adopted to determine the degree of suspicion for lung involvement in SARS-CoV-2 infection [66]. In the current study, on performing CT, almost all the recruited patients had PCR-proven SARS-CoV-2 infection, as described elsewhere [67]. Hence, they were classified as CORADS category six.…”

Section: Discussionmentioning

confidence: 98%

Assessment and outcome of hospitalized patients during delta variant COVID-19 pandemic: A multicenter international study

Amer

Saeed

Shaltout

et al. 2022

J Infect Dev Ctries

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Introduction: To date, the world has experienced four waves of the Coronavirus disease- 19 (COVID-19) pandemic. Patients infected during the era of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Delta variant were the subject of this study. The objectives were to describe their clinical manifestations, explain their laboratory and radiological findings, conclude factors contributing to clinical outcomes, and evaluate treatment protocols. Methodology: Relevant data were collected retrospectively from records of patients admitted to six referral centers in four countries. Data included sociodemographic patterns, symptoms, associated comorbidities, physical examination, laboratory and radiologic findings, treatment received, and patient outcomes. Results: Data analysis identified symptomatology and variables related to acquisition and infection outcome. The most prevalent symptoms were cough (81.5%), body aches (74.1%), and fever (71.6%). Independent risk factors for mortality were age, vomiting, epigastric pain, diabetes, obesity, oxygen saturation less than 90%, leukocytosis, neutrophilia, lymphopenia, thrombocytopenia, elevated creatinine, high glucose level, lung ground glass opacities with consolidation, affection of four lobes and bilateralism. Neither d-dimer nor lactate dehydrogenase nor ferritin foretells death possibility. The efficacy of the medications used was convenient. Conclusions: Assessing the clinical features of different COVID-19 waves, identifying predictors of outcomes, and concluding the efficacy of treatment protocols provide insight into patients’ responses and viral behaviors, which help in the proper diagnosis and treatment of subsequent surges.

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Section: Discussionmentioning

confidence: 98%

Assessment and outcome of hospitalized patients during delta variant COVID-19 pandemic: A multicenter international study

Amer

Saeed

Shaltout

et al. 2022

J Infect Dev Ctries

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“…It should be noted that Delta (B.1.617.2) and Omicron (B.1.1.529) variants were first reported in Egypt on August 24 and December 21, 2021, respectively, based on the WHO weekly epidemiologic updates [ 62 ], and started to circulate from then, which synchronized with our study and follow-up period. However, as described before [ 15 ], tracing the S gene expression status using TaqPath™ COVID-19 assay (Thermo Fischer Scientific) was an alternative route to identify SARS-CoV-2 variants [ 63 ]. A second limitation was the lack of a control arm; however, it was unattainable to recruit HCWs who could not be vaccinated and continue for nine months (the study duration) as a control arm without vaccination during the current COVID-19 pandemic.…”

Section: Discussionmentioning

confidence: 99%

“…After completing the vaccine schedule, participants were followed up for a further six months. Any incidence of breakthrough COVID-19 infection was reported during the study period, and all COVID-19 infections were diagnosed clinically and confirmed by laboratory investigations, chest imaging, and nucleic acid testing as described before [ 15 ]. At the conclusion of the study, all dropout subjects were excluded from statistical analysis ( Figure 1 ).…”

Section: Methodsmentioning

confidence: 99%

Maintenance of Antibody Response in Egyptian Healthcare Workers Vaccinated with ChAdOx1 nCoV-19 Vaccine during Delta and Omicron Variants Pandemic: A Prospective Study

et al. 2022

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Background: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a constantly evolving virus, resulting in an increased burden on the existing COVID-19 vaccines. Healthcare workers (HCWs) are the first line of defense against the coronavirus disease 2019 (COVID-19) pandemic and have been prioritized among the risk categories receiving the COVID-19 vaccine. This work aimed to investigate the maintenance of antibody response of the Oxford–AstraZeneca vaccine (ChAdOx1/nCoV-19). Methods: Anti-spike immunoglobulin G (IgG) was measured at baseline point (immediately prior to vaccination) and 12- and 24-week (w) points following vaccination. Adverse reactions to the vaccine were reported. Participants were followed up for the incidence of COVID-19 during the 12 w interval between vaccination doses for 24 w after the second dose. Results: A total of 255 HCWs participated in the study. Prior to vaccination, 54.1% experienced COVID-19, 88.2% were seropositive after the first dose, while seropositivity reached 95.7% after the second dose. Following the first and second doses, the anti-spike IgG serum level was significantly higher in subjects with past COVID-19 than in others (p < 0.001 and =0.001, respectively). Conclusions: The Oxford–AstraZeneca vaccine is generally safe and provides a highly effective long-term humoral immune response against the Delta and Omicron variants of SARS-CoV-2.

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“…Indonesia still has confirmed positive cases of COVID-19 across all provinces in the country (Widiawaty, et al, 2022;Azizah, et al, 2021). For identifying cases, the Real-Time Polymerase Chain Reaction Assay (RT-PCR) was used (Hatami, 2021;Hammad, et al, 2022). A sharp increase in the number of cases has been confirmed in the areas of East Java (Surabaya is the capital city), South Kalimantan, and South Sulawesi.…”

Section: Introductionmentioning

confidence: 99%

Knowledge, Perceived Self-care Ability, and Health Behavior in New Normal Era among Adults in Congested Communities of Surabaya, Indonesia: Impacts of COVID-19 Pandemic Situation

Sari

Artsanthia

2022

JEHCP

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Health behavior in COVID-19 pandemic situation needs to be measured in the general population in order to evaluate the implementation of health guidelines in the new normal era, especially WHO recommendations. Knowledge, attitude, and action are domains of health behavior that translated into COVID-19 knowledge, perceived self-care ability, and new normal health behavior in this study. We aimed at describing the knowledge of COVID-19 and the perceived self-care ability, and analyzing the differences of new normal health behavior before and after COVID-19 pandemic situation among adults living in congested communities of Surabaya, Indonesia. This cross-sectional study involved 384 respondents living in five congested communities of Surabaya, Indonesia, which enrolled by means of cluster random sampling. Research variables were measured by a self-developed valid and reliable questionnaires. Descriptive statistics and Wilcoxon signed rank test were used in data analysis. Ethical clearance was issued. Results showed that most respondents had very good knowledge of COVID-19 (63%) and high level of perceived self-care ability (74%). Before and after pandemic most respondents reported sufficient new normal health behavior (before: 74.7%; after: 83.9%). There was a significant difference of new normal health behavior before and after COVID-19 pandemic situation among adults living in congested communities of Surabaya, Indonesia (p=0.000). New normal health behavior was unoptimal in implementation. Therefore, efforts to improve new normal health behavior need to be done by various parties, not only the individuals, but also health care professionals and the government as policy maker.

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RT- PCR testing of upper respiratory tract samples for diagnosis of SARS-CoV-2: Between justification and overestimation, a multi-center international study

Cited by 3 publications

References 46 publications

Assessment and outcome of hospitalized patients during delta variant COVID-19 pandemic: A multicenter international study

Assessment and outcome of hospitalized patients during delta variant COVID-19 pandemic: A multicenter international study

Maintenance of Antibody Response in Egyptian Healthcare Workers Vaccinated with ChAdOx1 nCoV-19 Vaccine during Delta and Omicron Variants Pandemic: A Prospective Study

Knowledge, Perceived Self-care Ability, and Health Behavior in New Normal Era among Adults in Congested Communities of Surabaya, Indonesia: Impacts of COVID-19 Pandemic Situation

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