RTS1—an eukaryotic terminator of replication

Vengrova, Sonya; Codlin, Sandra; Dalgaard, Jacob Z.

doi:10.1016/s1357-2725(02)00040-7

Cited by 16 publications

(11 citation statements)

References 11 publications

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“…Replication checkpoint studies have typically used chemical agents or DNA replication mutants to stall replication, but there is abundant evidence of natural replication pause and termination sites (60). One of the best-characterized examples involves cell differentiation in fission yeast.…”

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confidence: 99%

Swi1 and Swi3 Are Components of a Replication Fork Protection Complex in Fission Yeast

Noguchi¹,

Noguchi²,

McDonald

et al. 2004

Molecular and Cellular Biology

194

314

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Swi1 is required for programmed pausing of replication forks near the mat1 locus in the fission yeast Schizosaccharomyces pombe. This fork pausing is required to initiate a recombination event that switches mating type. Swi1 is also needed for the replication checkpoint that arrests division in response to fork arrest. How Swi1 accomplishes these tasks is unknown. Here we report that Swi1 copurifies with a 181-amino-acid protein encoded by swi3؉ . The Swi1-Swi3 complex is required for survival of fork arrest and for activation of the replication checkpoint kinase Cds1. Association of Swi1 and Swi3 with chromatin during DNA replication correlated with movement of the replication fork. swi1⌬ and swi3⌬ mutants accumulated Rad22 (Rad52 homolog) DNA repair foci during replication. These foci correlated with the Rad22-dependent appearance of Holliday junction (HJ)-like structures in cells lacking Mus81-Eme1 HJ resolvase. Rhp51 and Rhp54 homologous recombination proteins were not required for viability in swi1⌬ or swi3⌬ cells, indicating that the HJ-like structures arise from single-strand DNA gaps or rearranged forks instead of broken forks. We propose that Swi1 and Swi3 define a fork protection complex that coordinates leading-and lagging-strand synthesis and stabilizes stalled replication forks.

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confidence: 99%

Swi1 and Swi3 Are Components of a Replication Fork Protection Complex in Fission Yeast

Noguchi¹,

Noguchi²,

McDonald

et al. 2004

Molecular and Cellular Biology

194

314

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“…In the fission yeast, it has been demonstrated that a defined RFB, the RTS1 element from the mat locus (25,26), can serve as a mitotic recombination hotspot in a RFB-dependent fashion (27,28), providing firm evidence that RFBs in mitotically dividing cells can drive genetic change. The RFB activity of the fission yeast RTS1 element has been shown to be dependent on a number of trans-acting proteins, 2 of which, Swi1 and Swi3, are the homologues of human TIMELESS and TIPIN, respectively (25).…”

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confidence: 99%

Recombination at DNA replication fork barriers is not universal and is differentially regulated by Swi1

Pryce

Ramayah²,

Jaendling³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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DNA replication stress has been implicated in the etiology of genetic diseases, including cancers. It has been proposed that genomic sites that inhibit or slow DNA replication fork progression possess recombination hotspot activity and can form potential fragile sites. Here we used the fission yeast, Schizosaccharomyces pombe, to demonstrate that hotspot activity is not a universal feature of replication fork barriers (RFBs), and we propose that most sites within the genome that form RFBs do not have recombination hotspot activity under nonstressed conditions. We further demonstrate that Swi1, the TIMELESS homologue, differentially controls the recombination potential of RFBs, switching between being a suppressor and an activator of recombination in a sitespecific fashion.fission yeast ͉ genome stability ͉ TIMELESS

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“…Reb1 binds to the Ter2 and Ter3 sites to cause polar fork arrest, and the magnitude of fork arrest at Ter3 is consistently greater than that at Ter2 (30,52). Rtf1 binds to the RTS1 site located near the mating type locus and also causes polar fork arrest (56). Reb1 and Rtf1 belong to the myb family of proteins, which contain twin canonical myb/ SANT (SANT is an acronym for the transcription factors Swi1 Ada2, NCOR, and TFIIIB) domains that are known to be involved in DNA binding (1,39) and/or interaction with histone tails (9,35).…”

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confidence: 99%

Mechanistic Insights into Replication Termination as Revealed by Investigations of the Reb1-Ter3 Complex of Schizosaccharomyces pombe

Biswas

Bastia

2008

Molecular and Cellular Biology

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Relatively little is known about the interaction of eukaryotic replication terminator proteins with the cognate termini and the replication termination mechanism. Here, we report a biochemical analysis of the interaction of the Reb1 terminator protein of Schizosaccharomyces pombe, which binds to the Ter3 site present in the nontranscribed spacers of ribosomal DNA, located in chromosome III. We show that Reb1 is a dimeric protein and that the N-terminal dimerization domain of the protein is dispensable for replication termination. Unlike its mammalian counterpart Ttf1, Reb1 did not need an accessory protein to bind to Ter3. The two myb/SANT domains and an adjacent, N-terminal 154-amino-acid-long segment (called the myb-associated domain) were both necessary and sufficient for optimal DNA binding in vitro and fork arrest in vivo. The protein and its binding site Ter3 were unable to arrest forks initiated in vivo from ars of Saccharomyces cerevisiae in the cell milieu of the latter despite the facts that the protein retained the proper affinity of binding, was located in vivo at the Ter site, and apparently was not displaced by the "sweepase" Rrm3. These observations suggest that replication fork arrest is not an intrinsic property of the Reb1-Ter3 complex.

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RTS1—an eukaryotic terminator of replication

Cited by 16 publications

References 11 publications

Swi1 and Swi3 Are Components of a Replication Fork Protection Complex in Fission Yeast

Swi1 and Swi3 Are Components of a Replication Fork Protection Complex in Fission Yeast

Recombination at DNA replication fork barriers is not universal and is differentially regulated by Swi1

Mechanistic Insights into Replication Termination as Revealed by Investigations of the Reb1-Ter3 Complex of Schizosaccharomyces pombe

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