Ru(bpy)<sub>3</sub><sup>2+</sup>‐doped Silica Nanoparticle Aptasensor for Detection of Thrombin Based on Electrogenerated Chemiluminescence

Wang, Xiaoying; Yun, Wen; Zhou, Jingming; Dong, Ping; He, Pingang; Fang, Yuzhi

doi:10.1002/cjoc.200890061

Cited by 23 publications

(15 citation statements)

References 48 publications

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“…Recently, various ECL biosensors using Ru(bpy) 3 2+ doped silica nanoparticles (RuDS nanoparticles) were described for genomic [72], proteomic analysis [73-75], and medical assay [76-78]. …”

Section: Nanomaterials As Carrier Of Ecl Probesmentioning

confidence: 99%

Applications of Nanomaterials in Electrogenerated Chemiluminescence Biosensors

Peng

Gao

et al. 2009

Sensors

108

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Electrogenerated chemiluminescence (also called electrochemiluminescence and abbreviated ECL) involves the generation of species at electrode surfaces that then undergo electron-transfer reactions to form excited states that emit light. ECL biosensor, combining advantages offered by the selectivity of the biological recognition elements and the sensitivity of ECL technique, is a powerful device for ultrasensitive biomolecule detection and quantification. Nanomaterials are of considerable interest in the biosensor field owing to their unique physical and chemical properties, which have led to novel biosensors that have exhibited high sensitivity and stability. Nanomaterials including nanoparticles and nanotubes, prepared from metals, semiconductor, carbon or polymeric species, have been widely investigated for their ability to enhance the efficiencies of ECL biosensors, such as taking as modification electrode materials, or as carrier of ECL labels and ECL-emitting species. Particularly useful application of nanomaterials in ECL biosensors with emphasis on the years 2004-2008 is reviewed. Remarks on application of nanomaterials in ECL biosensors are also surveyed.

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Section: Nanomaterials As Carrier Of Ecl Probesmentioning

confidence: 99%

Applications of Nanomaterials in Electrogenerated Chemiluminescence Biosensors

Peng

Gao

et al. 2009

Sensors

108

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“…Under the optimum conditions, the hemagglutinin molecules could be determined in a concentration range from 3 ¥ 10 -13 to 4 ¥ 10 -11 g/mL. Wang et al 73,74 prepared Ru(bpy)3 2+ doped silica nanoparticles. With a core-shell structure, Ru(bpy)3 2+ molecules incorporated into a silica matrix, could be prevented from entering the surrounding environment and the ECL intensity could be enhanced due to an increase in the number of Ru(bpy)3 2+ doped per nanoparticle.…”

Section: ·6 Electrochemiluminescence (Ecl)mentioning

confidence: 99%

Chemiluminescence Platforms in Immunoassay and DNA Analyses

Fan

Cao

et al. 2009

ANAL. SCI.

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IntroductionCL is the production of electromagnetic radiation by a chemical reaction. The CL intensity is directly proportional to the concentration of a limiting reactant involved in the CL reaction. CL has been exploited for its use in a wide range of applications due to its high sensitivity, wide calibration range, and suitability for miniaturization. Protein and DNA analyses have recently attracted much attention. Many different types of techniques, such as electrochemical, optical, radiochemical, and piezoelectronic methods have been applied for protein and DNA analyses. To provide an overview of CL detection assays for DNAs and proteins, we have summarized their essential techniques. Monoplex and multiplex assays are described, and their current reports are intended to provide an overview of the CL techniques currently employed in numerous laboratories. Monoplex AssayA monoplex assay aims to measure the presence of a single analyte in any given sample, in which nanoparticle, DNA enzyme, horseradish peroxidase (HRP), alkaline phosphatase (AP) and acridinium ester are commonly used as labels. 2·1 Nanoparticle-based CL techniquesNanoparticles offer excellent prospects for DNA detection and immunoassay, owing to their many attractive properties. Compared to existing labels, nanoparticles are more stable and cheaper, and easier to be purified. In addition, they indicate faster binding kinetics with high sensitivity and a high reaction rate for many types of monoplex assays, ranging from immunoassays to DNA detection. 2·1·1 Gold nanoparticlesColloidal gold labels are ideal in biotechnological systems for several reasons. Firstly, they can be readily prepared in a wide range of sizes, from approximately 2 nm to above 100 nm. Secondly, the biochemical activity of the labeled biomolecules can be retained when colloidal gold is coupled to the biomolecules and, thirdly, colloidal gold labels can be easily visualized as dense structures within biological entities using transmission electron microscopy. As a biological tag, colloidal gold has been employed in several CL detection systems. 2·1·1·1 Gold nanoparticle-based CL immunoassay. The Au 3+ -luminol reaction, in which 10 -9 M Au 3+ ions can be sensitively detected, is a classic CL reaction. Thousands of Au atoms are contained within the gold nanoparticles; for example, 2.3 ¥ 10 5 Au atoms are theoretically contained in a 20-nm spherical gold particle and, consequently, picomolar detection limits can be attained by employing oxidative gold metal dissolution in acidic solutions. Our group 1,2 reported a sensitive CL immunoassay based on a colloidal gold label after oxidative gold metal dissolution (using 0.01 M HCl-0.5 M NaCl-0.5 mM Br2), using a simple and sensitive luminol-CL reaction (Fig. 1). Reviews Chemiluminescence Platforms in Immunoassay and DNA AnalysesAiping FAN,* Zhijuan CAO,* Huan LI,* Masaaki KAI,** and Jianzhong LU* † *School of Pharmacy, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China ** Faculty of Pharmaceutical Sciences, Graduate Scho...

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“…The combination of nanotechnology and biotechnology for the detection of biological elements has brought new opportunities. Dye-doped silica nanoparticles [23][24][25] and Ru(bpy) 3 2+ -doped silica nanoparticles have been developed for immunochromatographic assays or electrogenerated chemiluminescence (ECL) [26]. In this work, a redox nanoparticle based on the Fc-COOH-doped SiNPs with a core-shell structure incorporated Fc-COOH core in the silica matrix shell was synthesized for the first time.…”

Section: Introductionmentioning

confidence: 99%

Ferrocenyl-doped silica nanoparticles as an immobilized affinity support for electrochemical immunoassay of cancer antigen 15-3

Hong

Yuan

Zhuo

2009

Analytica Chimica Acta

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Ru(bpy)₃²⁺‐doped Silica Nanoparticle Aptasensor for Detection of Thrombin Based on Electrogenerated Chemiluminescence

Cited by 23 publications

References 48 publications

Applications of Nanomaterials in Electrogenerated Chemiluminescence Biosensors

Applications of Nanomaterials in Electrogenerated Chemiluminescence Biosensors

Chemiluminescence Platforms in Immunoassay and DNA Analyses

Ferrocenyl-doped silica nanoparticles as an immobilized affinity support for electrochemical immunoassay of cancer antigen 15-3

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Ru(bpy)32+‐doped Silica Nanoparticle Aptasensor for Detection of Thrombin Based on Electrogenerated Chemiluminescence

Cited by 23 publications

References 48 publications

Applications of Nanomaterials in Electrogenerated Chemiluminescence Biosensors

Applications of Nanomaterials in Electrogenerated Chemiluminescence Biosensors

Chemiluminescence Platforms in Immunoassay and DNA Analyses

Ferrocenyl-doped silica nanoparticles as an immobilized affinity support for electrochemical immunoassay of cancer antigen 15-3

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Ru(bpy)₃²⁺‐doped Silica Nanoparticle Aptasensor for Detection of Thrombin Based on Electrogenerated Chemiluminescence