RU486 Suppresses Progesterone-induced Acrosome Reaction in Boar Spermatozoa

Jang, Sang Ho; Yi, Lee S. H.

doi:10.5483/bmbrep.2002.35.6.604

Cited by 8 publications

(8 citation statements)

References 19 publications

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“…Lately, non-genomic mPRs have been reported in conjunction with non-genomic functions of progesterone (Zhu et al 2003). In mammalian reproduction, progesterone has been implicated to be involved in AR (reviewed by Calogero et al 2000), in human (Osman et al 1989, Baldi et al 1995, Patrat et al 2000, mouse (Melendrez et al 1994), stallion (Meyers et al 1995), golden hamster (Meizel et al 1990), dog (Sirivaidyapong et al 1999), goat (Somanath et al 2000 and boar (Jang & Yi 2002). Progesterone, by binding to a non-genomic mPR, has been reported to induce AR via intracellular signal transduction cascades (Kopf 2002).…”

Section: Discussionmentioning

confidence: 99%

“…The AR can be induced by follicular fluid, cumulus cells (Tesarik et al 1993) or the ZP (Cross et al 1988). Identified as the active component of follicular fluid, progesterone, a steroid hormone known to induce transcriptional activations in the nucleus mediated by an intracellular receptor, has been reported to induce the AR in many mammalian spermatozoa (Osman et al 1989, Meizel et al 1990, Melendrez et al 1994, Baldi et al 1995, Meyers et al 1995, Sirivaidyapong et al 1999, Patrat et al 2000, Somanath et al 2000, Jang & Yi 2002, which strongly implies that progesterone exerts its effect on mammalian spermatozoa non-genomically via a still unidentified membrane-bound progesterone receptor (mPR). Although the mechanism is poorly understood, the AR induced by progesterone is regulated by signal transduction processes upon progesterone binding onto a still unidentified mPR.…”

Section: Introductionmentioning

confidence: 99%

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Identification of a 71 kDa protein as a putative non-genomic membrane progesterone receptor in boar spermatozoa

Jang¹,

Yi²

2005

Journal of Endocrinology

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A putative non-genomic progesterone receptor was identified by Western blot analysis from the membrane fraction but not the cytosolic fraction of boar spermatozoa using monoclonal antibody (mAb) C-262. When the membrane and the cytosolic fractions of boar liver, kidney, uterus and spermatozoa were analyzed with mAb C-262, protein bands with molecular masses of 86 and 120 kDa were detected from the cytosolic fraction of the uterus, whereas a 71 kDa protein was detected from the membrane fraction of spermatozoa. Apparently, while the 86 and 120 kDa proteins from the uterus correspond to the genomic progesterone receptor isoforms A and B in boar, the 71 kDa protein of the sperm membrane fraction seems to be a novel membrane-associated progesterone receptor. Ligand blot assay of the membrane and the cytosolic fractions of boar spermatozoa performed with peroxidaseconjugated progesterone revealed that only the 71 kDa membrane protein binds specifically to progesterone, reinforcing the results obtained from the Western blot analysis. Also ligand blot assays performed in the presence of mAb C-262 demonstrated that mAb C-262 inhibited progesterone binding to the 71 kDa protein in a dosedependent manner. Ligand blot assays performed in the presence of free progesterone, RU486 or estrogen revealed that binding of peroxidase-conjugated progesterone to the 71 kDa protein was inhibited by free progesterone and RU486 in a dose-dependent manner but not by estrogen, which further confirms that progesterone binds to the 71 kDa protein specifically. Furthermore, the progesterone-induced acrosome reaction was inhibited by mAb C-262 in a dose-dependent manner. These results strongly imply that spermatozoa possess a progesterone receptor in a membrane-bound form and can be influenced by progesterone via non-genomic progesterone receptor.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of a 71 kDa protein as a putative non-genomic membrane progesterone receptor in boar spermatozoa

Jang¹,

Yi²

2005

Journal of Endocrinology

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“…To examine the effect of the tannins on AR induction of capacitated sperm, a portion of the preincubated sperm was treated with 10 µg/ml progesterone in the presence or absence of 5 µg/ml of the tannins for 30 min at 39 C in an atmosphere of 5% CO 2 in air to induce AR as described previously [25]. Other preincubated sperm were coincubated with cumulus-free oocytes or extensively pretreated oocytes with 500 units/ml h y a l u r o n i d a s e f o r 2 h i n I V F m e d i u m supplemented with or without 5 µg/ml of the tannins for 2 h, and then sperm-oocyte complexes were gently rinsed several times with PBS using a wide-bore pipette to remove loosely attached spermatozoa.…”

Section: Evaluation Of Sperm Acrosome Statusmentioning

confidence: 99%

Antihyaluronidase Action of Ellagic Acid Effectively Prevents Polyspermy As a Result of Suppression of the Acrosome Reaction Induced by Sperm-Zona Interaction During In Vitro Fertilization of Porcine Oocytes

Tokeshi

Yoshimoto

Muto

et al. 2007

Journal of Reproduction and Development

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Abstract. The present study was conducted to examine the effects of three tannin relatives (tannic acid, TA; gallic acid, GA; and ellagic acid, EA) on antihyaluronidase and reactive oxygen species (ROS) scavenging activity, in vitro fertilization (IVF) parameters, and the acrosome reaction (AR) induced by sperm-zona interaction. Among the three tannin relatives, TA and EA showed the strongest potency for blocking the hyaluronidase activity of boar sperm, with concentrationdependent inhibition over the range of 2-10 µg/ml. In contrast, ROSs were effectively scavenged by TA and GA, but not EA. When cumulus-free oocytes were inseminated in IVF medium containing 5 µg/ml of the tannin relatives, polyspermy was significantly reduced by TA and EA (32 and 29%, respectively) compared with oocytes treated with or without GA (51 and 69%, respectively) under conditions that maintained a high sperm penetration rate (P<0.05). Interestingly, induction of the AR by treatment of preincubated sperm with progesterone was blocked by TA and GA as a result of their higher levels of ROS scavenging activity, while EA, which possessed weak ROS scavenging activity, did not disturb induction of the AR with progesterone. However, the incidence of AR induced by sperm-zona interaction was significantly decreased by the strong antihyaluronidase actions of TA and EA compared with that in the absence of these compounds. Treatment with the compounds caused neither a protective proteolytic modification of the zona pellucida matrix before fertilization nor a reduction in acrosomal proteolytic activity or the number of zona-bound sperm. These findings suggest that the antihyaluronidase action of EA effectively prevents polyspermy by suppression of AR functionality induced by sperm-zona interaction and that hyaluronidase intervention is therefore required during porcine IVF.

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“…As prematurely acrosome reacted sperm cannot undertake fertilisation [42] and progesterone, at micromolar concentrations, has previously been reported to cause the acrosome reaction in capacitated boar sperm [21,22] we sought to assess if the enhanced swim rate would be compromised by a parallel increase in the acrosome reaction. We demonstrated that the acrosome reaction is increased in the population of capacitated cells treated with 100nM progesterone.…”

Section: Discussionmentioning

confidence: 99%

“…Studies examining the effects of progesterone on boar sperm cells have largely focussed on its effects on the acrosome reaction [21][22][23]. However, high (micromolar) concentrations have been reported to increase motility [24] and intracellular calcium levels [14] in capacitated cells.…”

Section: Introductionmentioning

confidence: 99%

Progesterone significantly enhances the mobility of boar spermatozoa

Campbell¹,

Savage²,

Madamidola³

et al. 2013

Bidiscovery

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Progesterone released from the cumulus cells of the oocyte causes a number of physiological responses in human sperm cells including hyperactivation, acrosome reaction and chemotaxis. We employed a validated sperm mobility assay, which involves measuring the ability of sperm to penetrate an inert cell separation solution over time, to assess the ability of progesterone to enhance the mobility of boar spermatozoa. Cells maximally penetrate the solution over 50 minutes. 100nM progesterone significantly (P = 0.01) increased the mobility of non-capacitated sperm cells causing a doubling in the rate at which the cells penetrated through the cell separation solution (control half maximal penetration rate [Km] = 18.0±2.2; +100nM progesterone Km = 8.8±0.8min). Similarly, capacitated cells penetrated at a rate (Km = 19.2±3.0 min) not significantly different from non-capacitated cells and 100nM progesterone also significantly increased the rate of penetration of capacitated cells (Km = 9.5±1.0 min, P<0.05). The T-type voltage gated calcium channel blocker mibefradil (30mM) significantly inhibited both the control and progesterone enhanced mobility in non-capacitated and capacitated sperm. Only capacitated cells showed a significant increase in the acrosome reaction in response to 100nM progesterone (control non-reacted = 75±4%, +100nM progesterone nonreacted = 47±10%). Western blot analysis confirmed that there was an increase in the total protein tyrosine phosphorylation levels in capacitated cells. In conclusion, we have demonstrated that 100nM progesterone accelerates the mobility of boar sperm cells through an inert cell separation solution in an extracellular calcium dependent manner.

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RU486 Suppresses Progesterone-induced Acrosome Reaction in Boar Spermatozoa

Cited by 8 publications

References 19 publications

Identification of a 71 kDa protein as a putative non-genomic membrane progesterone receptor in boar spermatozoa

Identification of a 71 kDa protein as a putative non-genomic membrane progesterone receptor in boar spermatozoa

Antihyaluronidase Action of Ellagic Acid Effectively Prevents Polyspermy As a Result of Suppression of the Acrosome Reaction Induced by Sperm-Zona Interaction During In Vitro Fertilization of Porcine Oocytes

Progesterone significantly enhances the mobility of boar spermatozoa

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