Rubbed Films of Functionalized Bovine Serum Albumin as Substrates for the Imaging of Protein-Receptor Interactions Using Liquid Crystals

Kim, S.-R.; Abbott, Nicholas L.

doi:10.1002/1521-4095(200110)13:19<1445::aid-adma1445>3.0.co;2-9

Cited by 53 publications

(44 citation statements)

References 7 publications

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“…[5,6] These bioinspired, smart materials are attracting more and more interest because of their unique properties, which have paved the way to many real-world applications, e.g., biomimetic fins, [7] actively moving polymers, [8] neural memory devices, [9] smart micro-/nanocontainers for drug delivery, [10] various biosensors, [11][12][13] dual/multi-responsive materials. [14,15] Also, many of these smart materials have surfaces that dynamically alter their physicochemical properties in response to changes in their environmental conditions and a triggered control of interfacial properties at the solid/water interface can be found in ion channels, [13] directional surface motions, [16] and bioinspired, smart surfaces with controllable wettability and adhesion.…”

Section: Introductionmentioning

confidence: 99%

Bio‐Inspired, Smart, Multiscale Interfacial Materials

2008

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In this review a strategy for the design of bioinspired, smart, multiscale interfacial (BSMI) materials is presented and put into context with recent progress in the field of BSMI materials spanning natural to artificial to reversibly stimuli‐sensitive interfaces. BSMI materials that respond to single/dual/multiple external stimuli, e.g., light, pH, electrical fields, and so on, can switch reversibly between two entirely opposite properties. This article utilizes hydrophobicity and hydrophilicity as an example to demonstrate the feasibility of the design strategy, which may also be extended to other properties, for example, conductor/insulator, p‐type/n‐type semiconductor, or ferromagnetism/anti‐ferromagnetism, for the design of other BSMI materials in the future.

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Section: Introductionmentioning

confidence: 99%

Bio‐Inspired, Smart, Multiscale Interfacial Materials

2008

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“…For example, Kim et al demonstrated that the specific binding of anti-BSA IgG to the film of mechanically rubbed BSA erased the anisotropic topography induced within the film of BSA by rubbing, and caused an orientational transition of LCs from uniform to nonuniform state. 15 Luk et al investigated protein binding events on the obliquely deposited gold surface with nanometer-scale topographies. 16 The orientation of LCs changed from uniform to nonuniform state after the binding of proteins to the surface-immobilized receptor.…”

Section: -10mentioning

confidence: 99%

High-Contrast Imaging of Biomolecular Interactions Using Liquid Crystals Supported on Roller Printed Protein Surfaces

Park¹,

Jang²

2012

Bulletin of the Korean Chemical Society

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In this study, we report a new method for the high contrast imaging of biomolecular interactions at roller printed protein surfaces using thermotropic liquid crystals (LCs). Avidin was roller printed and covalently immobilized onto the obliquely deposited gold surface that was decorated with carboxylic acid-terminated self-assembled monolayers (SAMs). The optical response of LCs on the roller printed film of avidin contrasted sharply with that on the obliquely deposited gold surface. The binding of biotin-peroxidase to the roller printed avidin was then investigated on the obliquely deposited gold substrate. LCs exhibited a non-uniform and random orientation on the roller printed area decorated with the complex of avidin and biotin-peroxidase, while LCs displayed a uniform and planar orientation on the area without roller printed proteins. The orientational transition of LCs from uniform to non-uniform state was triggered by the erasion of nanometer-scale topographies on the roller printed surface after the binding of biotin-peroxidase to the surface-immobilized avidin. The specific binding events of protein-receptor interactions were also confirmed by atomic force microscopy and ellipsometry. These results demonstrate that the roller printing of proteins on obliquely deposited gold substrates could provide a high contrast signal for imaging biomolecular interactions using LCbased sensors.

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“…Optical sensors based on liquid crystals (LCs) have been investigated extensively in the past. For instance, a broad range of optical sensors was developed previously which are based on interfacial changes that influence the alignment of liquid crystals in response to analytes such as light, [2] gasses, [3][4][5][6] biomolecules [7][8][9] and aerosols [5] which is covered in an excellent recent review by Carlton et al [10] In these sensors, chemically functionalized self-assembled monolayers are employed which react with analytes that act as an alignment layer for liquid crystals. The change in birefringence is monitored upon exposure to an analyte via transmission measurements between crossed linear polarizers.…”

Section: Introductionmentioning

confidence: 99%

Optical Acetone Vapor Sensors Based on Chiral Nematic Liquid Crystals and Reactive Chiral Dopants

Cachelin

Green

Peijs

et al. 2016

Advanced Optical Materials

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Accurate monitoring of exposure to organic vapors, such as acetone, is an important part of maintaining a safe working environment and adhering to long-and short-term exposure limits.Here, a novel acetone vapor detection system is described based on the use of a reactive chiral dopant in a nematic liquid crystal thin film, which can accurately monitor the total exposure over a given timeframe through changes in the reflection band of the film. It was found that films exposed to 1000 parts per million by volume (ppmv) of acetone vapor for two hours exhibited a shift in reflection band of 12 nm, with smaller shifts observed at lower concentrations of acetone. Such sensors have potential applications in industrial hygiene.

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Rubbed Films of Functionalized Bovine Serum Albumin as Substrates for the Imaging of Protein-Receptor Interactions Using Liquid Crystals

Cited by 53 publications

References 7 publications

Bio‐Inspired, Smart, Multiscale Interfacial Materials

Bio‐Inspired, Smart, Multiscale Interfacial Materials

High-Contrast Imaging of Biomolecular Interactions Using Liquid Crystals Supported on Roller Printed Protein Surfaces

Optical Acetone Vapor Sensors Based on Chiral Nematic Liquid Crystals and Reactive Chiral Dopants

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