RuBi-Glutamate: Two-photon and visible-light photoactivation of neurons and dendritic spines

Fino, Élodie; Araya, Roberto; Peterka, Darcy S.; Salierno, Marcelo; Etchenique, Roberto; Yuste, Rafael

doi:10.3389/neuro.04.002.2009

Cited by 180 publications

(163 citation statements)

References 24 publications

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“…The design of RuBi-GABA [18] and RuBiGlutamate [20] opened a wide spectrum of possibilities to experiment with 1P and 2P active caged versions of the most ubiquitous inhibitory and excitatory neurotransmitters. RuBi-Glutamate appears to be a superb tool to determine circuitry in dense neuronal systems [28][29][30][31][32]. On the other hand, the physiology of neuronal inhibition can be studied with the use of RuBi-GABA, the first caged GABA with 2P capabilities, necessary to address single dendritic spines [33][34][35][36].…”

Section: Applications Of Ru-bpy Complexes In Neurophysiologymentioning

confidence: 99%

Ruthenium polypyridyl phototriggers: from beginnings to perspectives

Zayat

Filevich

Baraldo

et al. 2013

Phil. Trans. R. Soc. A.

104

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Octahedral Ru(II) polypyridyl complexes constitute a superb platform to devise photoactive triggers capable of delivering entire molecules in a reliable, fast, efficient and clean way. Ruthenium coordination chemistry opens the way to caging a wide range of molecules, such as amino acids, nucleotides, neurotransmitters, fluorescent probes and genetic inducers. Contrary to other phototriggers, these Ru-based caged compounds are active with visible light, and can be photolysed even at 532 nm (green), enabling the use of simple and inexpensive equipment. These compounds are also active in the two-photon regime, a property that extends their scope to systems where IR light must be used to achieve high precision and penetrability. The state of the art and the future of ruthenium polypyridyl phototriggers are discussed, and several new applications are presented.

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Section: Applications Of Ru-bpy Complexes In Neurophysiologymentioning

confidence: 99%

Ruthenium polypyridyl phototriggers: from beginnings to perspectives

Zayat

Filevich

Baraldo

et al. 2013

Phil. Trans. R. Soc. A.

104

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“…To confirm our results, we stimulated astrocytes with another single-cell resolution method using targeted two-photon uncaging of RuBi-glutamate (30). In these experiments, we recorded from a patched neuron in layer 2/3 following photostimulation of an SR101-labeled astrocyte in layer 1 (Fig.…”

mentioning

confidence: 99%

Astrocytic regulation of cortical UP states

Poskanzer

Yuste²

2011

Proc. Natl. Acad. Sci. U.S.A.

189

166

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The synchronization of neuronal assemblies during cortical UP states has been implicated in computational and homeostatic processes, but the mechanisms by which this occurs remain unknown. To investigate potential roles of astrocytes in synchronizing cortical circuits, we electrically activated astrocytes while monitoring the activity of the surrounding network with electrophysiological recordings and calcium imaging. Stimulating a single astrocyte activates other astrocytes in the local circuit and can trigger UP state synchronizations of neighboring neurons. Moreover, interfering with astrocytic activity with intracellular injections of a calcium chelator into individual astrocytes inhibits spontaneous and stimulated UP states. Finally, both astrocytic activity and neuronal UP states are regulated by purinergic signaling in the circuit. These results demonstrate that astroglia can play a causal role in regulating the synchronized activation of neuronal ensembles.T he transient synchrony of distributed groups of neurons is an important operational characteristic of the cerebral cortex for sensory processing and internal computational functions (1-4). One important type of synchrony-the multineuronal, network-driven fluctuations in membrane potential known as UP and DOWN states-occurs in neocortex both in vitro and in vivo (5-11). During UP states, neurons are depolarized for up to hundreds of milliseconds, sometimes firing barrages of action potentials (12). The function of these UP states is unknown, although they underlie synchronization among distant cortical territories (13). UP states in vivo are observed at similar frequencies as the "resting" spontaneous activity that is evident in functional MRI and EEG recordings from human subjects when not involved in sensory motor tasks (14-16), so they may be a cellular basis of this activity.The mechanisms by which UP states occur remain unclear, although recurrent excitatory activity is important (6, 9). Because astrocytes regulate extracellular glutamate (17), we explored their effects on cortical UP states using electrophysiology and population calcium imaging. The effects of astroglia on synaptic transmission and plasticity have been intensely studied, but their role in coherent function of the circuit has received much less attention, even though glia constitute almost half of all of the cells in the adult human brain (17) and are well-suited for longrange, network-wide signaling. Astrocytes also tile the cortex with near-complete coverage (18,19), are connected into an extensive syncytium via gap junctions (20,21), and communicate by intercellular calcium signaling (22), and processes from a single astrocyte can contact up to tens of thousands of synapses (18), making them attractive candidates for mediating neuronal synchronization. Here we describe a role of astrocytes in the cortical circuit, demonstrating their importance in the modulation of neuronal UP states.Results UP States Are Network-Wide Events. We performed whole-cell patch clamping of neurons i...

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“…Cette technique utilise un composé de glutamate « cagé », où le glutamate est couplé à une molécule (appelée molé-cule cage) le rendant ainsi biologiquement inactif et donc incapable de se fixer à ses récepteurs ( Figure 1A). Le RuBi-Glutamate [7], un composé déve-loppé récemment, s'avère la plus efficace des molécules utilisées jusqu'ici. En réponse à l'illumination du RuBiGlutamate par le laser, le glutamate [7,8] Figure 2A illustre un exemple de cartographies inhibitrices pour deux cellules pyramidales voisines non connectées.…”

Section: Identification De Réseaux Inhibiteurs Denses Dans Les Circuiunclassified

“…Le RuBi-Glutamate [7], un composé déve-loppé récemment, s'avère la plus efficace des molécules utilisées jusqu'ici. En réponse à l'illumination du RuBiGlutamate par le laser, le glutamate [7,8] Figure 2A illustre un exemple de cartographies inhibitrices pour deux cellules pyramidales voisines non connectées. On y observe la similitude des réseaux inhibiteurs pour ces deux cellules pyramidales voisines.…”

Section: Identification De Réseaux Inhibiteurs Denses Dans Les Circuiunclassified

Les connexions neuronales en pleine lumière

Fino

2012

Med Sci (Paris)

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RuBi-Glutamate: Two-photon and visible-light photoactivation of neurons and dendritic spines

Cited by 180 publications

References 24 publications

Ruthenium polypyridyl phototriggers: from beginnings to perspectives

Ruthenium polypyridyl phototriggers: from beginnings to perspectives

Astrocytic regulation of cortical UP states

Les connexions neuronales en pleine lumière

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