RuBisCO nano enzyme for mimicking CO₂ conversion system in plants

Abstract

In this study, carbon dioxide (CO2) capture and conversion systems based on the combination of biomimetic systems with nano enzymes have been developed. The effectiveness of the developed system has been investigated toward CO2 conversion. For this aim, nano ribulose bisphosphate carboxylase/oxygenase (RuBisCO) enzyme that plays role in the Calvin cycle in photosynthesis has been synthesized in 93 nm size according to AmiNoAcid (monomer) Decorated and Light Underpinning Conjugation Approach (ANADOLUCA) method. Enzymatic activity of synthesized nano RuBisCO enzyme has been spectrophotometrically determined by the formation of 3‐phosphoglycerate (3‐PGA) at the end of the reaction between CO2 and d‐ribulose‐1,5 biphosphate with the catalysis of RuBisCO enzyme at 340 nm. The effect of substrate concentration, pH, temperature, and Mg2+ ion concentration on the conversion reaction have investigated comparatively with nano and free RuBisCO enzyme. Besides this, the reusability feature of synthesized nano RuBisCO enzyme in conversion of CO2 reaction is indicated. When all data were evaluated, it has been seen that the nano RuBisCO enzyme is effective on the conversion of CO2 into 3‐PGA and can be used for CO2 capture and conversion systems repeatedly without any deformation in its structure.

Nanozyme as detector and remediator to environmental pollutants: between current situation and future prospective

“…Furthermore, the enzyme activity was gauged through spectrophotometric measurements, an advancement that bestows an additional benefit upon the devised system. As per the findings, the unbound RuBisCO enzyme exhibited an enzyme activity of 8.62 U/mg of substrate, while the nanoRuBisCO enzyme displayed an enzyme activity of 6.50 U/mg of substrate (Biçen Ünlüer et al 2021 ). According to various sampling dates, RuBisCO enzyme activity in tomato plants was reported as 4–9 U/mg substrate in the literature (Mitra et al 2019 ).…”