Rumen Bacterial Community Transition During Adaptation to High-grain Diet

Tajima, Kiyoshi; Arai, Shozo; Ogata, Koretsugu; Nagamine, Takafumi; Matsui, Hiroki; Nakamura, Mako; Aminov, Rustam; Benno, Yoshimi

doi:10.1006/anae.2000.0353

Cited by 262 publications

(256 citation statements)

References 16 publications

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“…The prime function of Succiniclasticum ruminis is to ferment succinate and quantitatively convert it to propionate. Succiniclasticum has been shown to exist in many ruminants, including yaks [17,23], cow [73], cattle [39,53], buffaloes [85] and goat [47]. In the present study, it was also found in Tibetan sheep.…”

Section: Core Prokaryotic Communities In the Rumensupporting

confidence: 63%

Rumen prokaryotic communities of ruminants under different feeding paradigms on the Qinghai-Tibetan Plateau

Xue

Chen

Zhao

et al. 2017

Systematic and Applied Microbiology

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Section: Core Prokaryotic Communities In the Rumensupporting

confidence: 63%

Rumen prokaryotic communities of ruminants under different feeding paradigms on the Qinghai-Tibetan Plateau

Xue

Chen

Zhao

et al. 2017

Systematic and Applied Microbiology

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“…Isolate I-6 T is most closely related, among published isolates, to HAP isolate C2 of Attwood et al (1998) (89 % identity), two asaccharolytic species, Eubacterium minutum and Eubacterium infirmum, from the mouth (Cheeseman et al, 1996;Wade et al, 1999a, b) (also 89 % identity), and another oral species, Eubacterium brachy (Hamid et al, 1994) (88 % identity). Its 16S rDNA sequence is very similar to three sequences obtained from the rumen by Tajima et al (2000) (accession nos AB034093, AB034092 and AB034148). These bacteria do not fall into any of the clusters proposed by Collins et al (1994), being taxonomically quite distant from the closest group, cluster XI (Attwood et al, 1998;Cheeseman et al, 1996).…”

Section: Identification and Phylogenetic Analysissupporting

confidence: 57%

Eubacterium pyruvativorans sp. nov., a novel non-saccharolytic anaerobe from the rumen that ferments pyruvate and amino acids, forms caproate and utilizes acetate and propionate

Wallace¹,

McKain²,

McEwan³

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

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Two similar Gram-positive rods were isolated from 10 "6 dilutions of ruminal fluid from a sheep receiving a mixed grass hay/concentrate diet, using a medium containing pancreatic casein hydrolysate as sole source of carbon and energy. The isolates did not ferment sugars, but grew on pyruvate or trypticase, forming caproate as the main fermentation product and valerate to a lesser extent. Acetate and propionate were utilized. One of these strains, I-6 T , was selected for further study. Strain I-6 T was a non-motile coccal rod, 1?260?4 mm, with a Gram-positive cell wall ultrastructure and a G+C content of 56?8 mol%. No spores were visible, and strain I-6 T did not survive heating at 80˚C for 10 min. Its rate of NH 3 production was 375 nmol (mg protein) "1 min "1 , placing it in the 'ammonia-hyperproducing' (or HAP) group of ruminal bacteria. 16S rDNA sequence analysis (1296 bases) indicated that it represents a novel species within the 'low-G+C' Grampositive group, for which the name Eubacterium pyruvativorans sp. nov. is proposed. Among cultivated bacteria, strain I-6 T was most closely related (89 % identity) to other asaccharolytic Eubacterium isolates from the mouth and the rumen. It was 98 % identical to uncultured bacterial sequences amplified by others from ruminal digesta.

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“…The predominance of Clostridium suggests that these organisms are closely associated with the decomposition of Microcystis biomass. As members of the genus Clostridium are capable of forming endospores, they are ubiquitous and can be found in many extreme environments, such as the deep sea, hot springs and the intestinal tracts of animals (Jin et al, 1988;Tajima et al, 2000;Van Dyke and McCarthy, 2002;Verberkmoes et al, 2009). Furthermore, microorganisms affiliated with Clostridium have been found to be major players in the fermentation of organic substances.…”

Section: Discussionmentioning

confidence: 99%

Novel Clostridium populations involved in the anaerobic degradation of Microcystis blooms

et al. 2010

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Understanding the microbial degradation of Microcystis biomass is crucial for determining the ecological consequences of Microcystis blooms in freshwater lakes. The purpose of this study was to identify bacteria involved in the anaerobic degradation of Microcystis blooms. Microcystis scum was anaerobically incubated for 90 days at three temperatures (15 1C, 25 1C and 35 1C). We used terminal restriction fragment length polymorphism (T-RFLP) analysis of bacterial 16S rRNA genes, followed by cloning and sequencing of selected samples, to reveal the community composition of bacteria and their dynamics during decomposition. Clostridium spp. were found to be the most dominant bacteria in the incubations, accounting for 72% of the sequenced clones. Eight new clusters or subclusters (designated CLOS.1-8) were identified in the Clostridium phylogenetic tree. The bacterial populations displayed distinct successions during Microcystis decomposition. Temperature had a strong effect on the dynamics of the bacterial populations. At 15 1C, the initial dominance of a 207-bp T-RF (Betaproteobacteria) was largely substituted by a 227-bp T-RF (Clostridium, new cluster CLOS.2) at 30 days. In contrast, at 25 1C and 35 1C, we observed an alternating succession of the 227-bp T-RF and a 231-bp T-RF (Clostridium, new cluster CLOS.1) that occurred more than four times; no one species dominated the flora for the entire experiment. Our study shows that novel Clostridium clusters and their diverse consortiums dominate the bacterial communities during anaerobic degradation of Microcystis, suggesting that these microbes' function in the degradation process.

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Rumen Bacterial Community Transition During Adaptation to High-grain Diet

Cited by 262 publications

References 16 publications

Rumen prokaryotic communities of ruminants under different feeding paradigms on the Qinghai-Tibetan Plateau

Rumen prokaryotic communities of ruminants under different feeding paradigms on the Qinghai-Tibetan Plateau

Eubacterium pyruvativorans sp. nov., a novel non-saccharolytic anaerobe from the rumen that ferments pyruvate and amino acids, forms caproate and utilizes acetate and propionate

Novel Clostridium populations involved in the anaerobic degradation of Microcystis blooms

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