Rumen Chemistry, In Vitro Studies with Rumen Microorganisms Using Carbon-14-Labeled Casein, Glutamic Acid, Leucine, and Carbonate

Otagaki, K. K.; Black, Arthur L.; Goss, Harold; Kleiber, Max

doi:10.1021/jf60057a007

Cited by 30 publications

(8 citation statements)

References 9 publications

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“…Several reports on the metabolism of glutamic acid by rumen microorganisms have described low recoveries of glutamate carbon. Otagaki et al (9) recovered only 37% of added carbon, Van den Hende, Oyaert, and Bouckaert (12) found 75% of the radioactivity from glutamate-i-C14 in carbon dioxide or volatile fatty acids, whereas Portugal recovered between 52.9 and 72.0% of the counts from glutamate-U-C'4. It is possible that part of the missing carbon was y-aminobutyric acid.…”

Section: Resultsmentioning

confidence: 99%

Amino Acid Concentrations in Rumen Fluid

Wright¹,

Hungate²

1967

Applied Microbiology

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Methods using dialysis or ultrafiltration are described for the collection of extracellular fluid in rumen contents for analysis of amino acids. Marked differences in the concentration of aspartic acid, glutamic acid, and alanine were found in samples of either diffusate or ultrafiltrate and in clarified acidified rumen liquor. Concentrations are given for aspartic acid, glutamic acid, alanine, glycine, 'y-aminobutyric acid, valine, 5-aminovaleric acid, and leucine.

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Section: Resultsmentioning

confidence: 99%

Amino Acid Concentrations in Rumen Fluid

Wright¹,

Hungate²

1967

Applied Microbiology

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“…The observed disappearance of lactate in three media may be explained by its conversion to propionate. Butyric acid has been shown to derive from several amino acids (7), whereas isobutyrate may originate from valine (2) and isovalerate from leucine (2,8). Catabolism of aspartate, serine, and threonine may generate lactic acid (7).…”

Section: Discussionmentioning

confidence: 99%

Effects of Time and Growth Media on Short-Chain Fatty Acid Production by Bacteroides fragilis

Mayhew¹,

Onderdonk²,

Gorbach³

1975

Applied Microbiology

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Gas-liquid chromatography was used to monitor the evolution of short chain fatty acids by Bacteroides fragilis in five media. Acetic and succinic acids, the prominent end products encountered, were readily detected within 24 h. Propionic, isobutyric, butyric, isovaleric, and lactic acids were usually recorded in more limited quantities. Maximum rates of bacterial multiplication, glucose catabolism, and end-product production coincided with the first 24 h in carbohydrate-supplemented media. Extended incubation (672 h) favored substantial succinate increases in three of five media. These observations suggest that incubation time and composition of the medium are important determinants in short chain fatty acid production by B. fragilis.

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“…Extensive fixation of CO2 into amino acids in rumen microorganisms has been demonstrated by several workers (22,24,30). R. flavefaciens fixed C02, but not formate, into the carboxyl carbon of leucine (4), but the mechanism of the carboxylation was not determined.…”

Section: Downloaded Frommentioning

confidence: 99%