Rumen fermentation and microbial community composition influenced by live Enterococcus faecium supplementation

Mamuad, Lovelia L.; Kim, Seon Ho; Biswas, Ashraf A.; Yu, Zhongtang; kwang-keun, cho; Kim, Sang-Bum; Lee, Kichoon; Lee, Sang-Suk

doi:10.1186/s13568-019-0848-8

Cited by 33 publications

(20 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, population of ruminal fiber degrading bacteria ( Ruminococcus and Fibrobacter ) showed no significant difference between FP and CON lambs. Contrary to our results in the in vitro experiment, the use of 0.1% E. faecium (7.0 × 10 8 cfu/mL) increased the ruminal population of R. flavefaciens and F. succinogenes compared to the control ( Mamuad et al, 2019 ). The increase in the population of fiber-degrading bacteria in treatments SCFP and MSCFP is probably due to the presence of Me and SC.…”

Section: Discussioncontrasting

confidence: 99%

See 1 more Smart Citation

Effect of microbial feed additives on growth performance, microbial protein synthesis, and rumen microbial population in growing lambs

Direkvandi¹,

Mohammadabadi²,

Salem

2020

Translational Animal Science

View full text Add to dashboard Cite

Arabi lambs (n =28; body weight = 24 ± 3.7 kg; average age = 120 ± 8 days) were used to investigate the effect of microbial additives on growth performance, microbial protein synthesis and rumen microbial population of fattening lamb based on completely randomized design. Four treatments were studied; (1) control (without additive; CON); (2) Lactobacillus fermentum and L. plantarum (FP); (3) Saccharomyces cerevisiae (SC) plus FP (SCFP) and (8) Megasphaera elsdenii plus SCFP (MSCFP). Lambs were inoculated before morning feeding (daily oral dosed) with a 50 mL microbial suspension as follow: FP, 50 mL bacterial suspension containing 4.5 × 10 8 colony-forming unit per day (cfu/day) of L. plantarum and L. fermentum (in ratio 50:50)); SCFP, 50 mL microbial suspension containing 4.5 × 10 8 cfu/day FP and 1.4 × 10 10 cfu/day SC; MSCFP, 50 mL microbial suspension containing 4.5 × 10 8 cfu/day Me, 4.5 × 10 8 cfu/day FP and 1.4 × 10 10 cfu/day SC. Feed intake and body weight of lambs were not affected by microbial additives. Average daily gain and feed efficiency were increased on day 0-21. The highest concentration of uric acid, total excreted purine derivatives (PD), microbial N, microbial CP and metabolizable protein were in MSCFP lambs. The ruminal population of Ruminococcus albus and Ruminococcus flavefaciens was higher in MSCFP and SCFP than CON and FP lambs. The highest and the lowest abundance of M. elsdenii and methanogen respectively was observed in lambs fed on microbial additives. The tendency to improve growth performance versus CON may be due to improvements in microbial protein synthesis and microbial populations, especially fiber-degrading bacteria. The decrease in the population of methanogens as a result of the use of microbial additives is another positive result.

show abstract

Section: Discussioncontrasting

confidence: 99%

“…The decrease in methanogen population may be due to the increase in F. succinogenes population as a result of microbial additives. Because F. succinogenes is a nonhydrogen producing bacteria, and hydrogen is a substrate for methanogens ( Mamuad et al, 2019 ), therefore, less substrate will be accessible for methanogens.…”

Section: Discussionmentioning

confidence: 99%

Effect of microbial feed additives on growth performance, microbial protein synthesis, and rumen microbial population in growing lambs

Direkvandi¹,

Mohammadabadi²,

Salem

2020

Translational Animal Science

View full text Add to dashboard Cite

show abstract

“…Because F. succinogenes is a non‐H 2 producing bacteria, and H 2 is a substrate for methanogens, therefore, less substrate will be accessible for methanogens (Mamuad et al . 2019). Waldrip and Martin (1993) reported that the fungal additive stimulates the LUB to uptake lactate but does not change the biofermentation profile.…”

Section: Discussionmentioning

confidence: 99%

Influence of three microbial feed additives of Megasphaera elsdenii , Saccharomyces cerevisiae and Lactobacillus sp. on ruminal methane and carbon dioxide production, and biofermentation kinetics

Direkvandi¹,

Mohammadabadi²,

Salem

2021

J Appl Microbiol

View full text Add to dashboard Cite

Aims: This study was performed to investigate the effects of Megasphaera elsdenii (Me), Saccharomyces cerevisiae (SC) and lactic acid bacteria (FP-Lactobacillus fermentum plus Lactobacillus plantarum) alone or in combination on biogas production and ruminal biofermentation parameter in a heterofermenter system. Methods and Results: Eight treatments were evaluated; (i) control (without additive; CON); (ii) Me; (iii) SC; (iv) FP; (v) Me plus SC (MSC); (vi) Me plus FP (MFP); (vii) SC plus FP (SCFP) and (viii) Me plus SC plus FP (MSCFP).Doses of FP, Me and SC were 1Á5 × 10 8 (CFU per ml), 1Á5 × 10 8 (CFU per ml) and 1Á4 × 10 7 (CFU 0Á002 -1 g), respectively. Biogas production in all time increased (P < 0Á05) by MSCFP than CON additive. The proportional methane (CH 4 ) decreased (P < 0Á05) in MSCFP and FP, while carbon dioxide (CO 2 ) was decreased (P < 0Á05) by SC compared MSCFP and MSC. The proportional CO 2 decreased (P < 0Á05) by MSCFP and FP additive. The mean concentration of NH 3 -N was not affected by treatments. Concentration of total volatile fatty acids and the percent of acetate and propionate was not affected by treatments. The highest (P < 0Á05) percent of butyrate and valerate were observed in MSCFP additive. The experiment showed that microbial additives of FP, SCFP and MSCFP reduced proportional CH 4 and CO 2 . Conclusions: Microbial additives of MFP and MSCFP had a sustainable positive efficiency on pH and volatile fatty acids and mitigate CH 4 and CO 2 . Significance and Impact of the Study: The use of microbial additives control on the ruminal pH (MFP) and improve VFA such as butyrate (MSC, MSCFP) and valerate (MSCFP) and reduce the greenhouse gases production showed a reduced risk of ruminal acidosis.

show abstract

“…The increase in cumulative gas production is due to the higher initial fermentation rate of the corn treatments. Ruminal NH 3 -N and pH at 0 and 72 h incubation were not altered by diets containing corn with PKC urea-treated rice straw based diets, ranging from 31-36 mg/dL and pH 6.69 -6.79 at 72 h. The level of ruminal NH 3 -N exceeded 5-8 mg/dL in all treatment groups, which is the optimal level of NH 3 -N for microbial protein synthesis (Mamuad et al 2019;Satter and Slyter 1974), also all treatment pH means were within the normal range with the values showing relative stability at 6.22-6.53, which is the optimal level for microbial digestion of ber and protein 6.0-7.0 (Chanjula et al 2010;Chen et al 2016;Van Soest 1994). Higher rumen NH 3 -N concentration of 36.79 mg/dL was observed at T1 while 35.69 mg/dL was recorded at T2.…”

Section: Discussionmentioning

confidence: 82%

Profiling of in vitro rumen digestibility, fermentation parameters and fatty acid biohydrogenation of palm kernel cake-based diet supplemented with corn

Saeed¹,

Sani²,

Sazili³

et al. 2021

Preprint

View full text Add to dashboard Cite

Corn supplementation can enhance the function of rumen and mitigate methane production. Thus, this study aimed to evaluate in vitro rumen digestibility, fermentation parameters and fatty acid biohydrogenation of palm kernel cake-based (PKC) diet substituted with different levels of corn. Corn was substitution into PKC basal diet at the levels; T1= (0% corn + 75.3% PKC), T2= (5% corn + 70.3% PKC) and T3= (10% corn + 65.3% PKC) of the diet. Rumen liquor was obtained from four fistulated Dorper sheep and incubated with 200 mg of each treatment for 24hrs and 72hrs. Net gas production, fermentation kinetics, in vitro organic matter digestibility (IVOMD), in vitro dry matter digestibility (IVDMD), volatile fatty acids (VFA), rumen microbial population and fatty acid biohydrogenation were determined. The results of the in vitro study showed that production of gas increased from 0 hr until 9 hrs with T2 having the highest gas production during this phase. After 48 hrs, the gas production began to decrease gradually with increase in incubation time. No significant differences were observed in the IVDMD, IVOMD, NH3-N, pH and VFA at 72 hrs. However, higher significant methane gas (CH4) production was observed in T3 when compared with T1 and T2. Microbial population did not differ significantly between treatment groups for total bacteria, F. succinogenes and R. flavefaciens. The rates of biohydrogenation were not affected by corn substitution although a significant difference was observed in that of C18:1n9. In conclusion, corn substitution maintained fermentation characteristics with increasing of unsaturated fatty acids.

show abstract

Rumen fermentation and microbial community composition influenced by live Enterococcus faecium supplementation

Cited by 33 publications

References 50 publications

Effect of microbial feed additives on growth performance, microbial protein synthesis, and rumen microbial population in growing lambs

Effect of microbial feed additives on growth performance, microbial protein synthesis, and rumen microbial population in growing lambs

Influence of three microbial feed additives of Megasphaera elsdenii , Saccharomyces cerevisiae and Lactobacillus sp. on ruminal methane and carbon dioxide production, and biofermentation kinetics

Profiling of in vitro rumen digestibility, fermentation parameters and fatty acid biohydrogenation of palm kernel cake-based diet supplemented with corn

Contact Info

Product

Resources

About