Rumen microbial abundance and fermentation profile during severe subacute ruminal acidosis and its modulation by plant derived alkaloids in vitro

Mickdam, Elsayed; Khiaosa‐ard, Ratchaneewan; Metzler‐Zebeli, Barbara U.; Klevenhusen, Fenja; Chizzola, Remigius; Zebeli, Qendrim

doi:10.1016/j.anaerobe.2016.02.002

Cited by 53 publications

(67 citation statements)

References 44 publications

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“…According to Forbes and Barrio, cellulose degradation is suppressed at osmolalities above 400 mOsmol kg −1 . In our study, the major fibrolytic bacteria (including the genera Fibrobactor , Ruminococcus , and Trepanoma ) were sensitive to hyperosmolality, whereas amylolytic bacteria, propionate producers, and acid‐tolerant bacteria (such as Prevotella , Lactobacillus , and Bifidobacterium ) proliferated, a characteristic that has been previously associated with high‐grain feeding . Consistently, we found that NDF degradation was suppressed to a greater extent by hyperosmolality than the degradation of NFCs was.…”

Section: Discussionsupporting

confidence: 88%

“…In our study, the major fibrolytic bacteria (including the genera Fibrobactor, Ruminococcus, and Trepanoma) were sensitive to hyperosmolality, whereas amylolytic bacteria, propionate producers, and acid-tolerant bacteria (such as Prevotella, Lactobacillus, and Bifidobacterium) proliferated, a characteristic that has been previously associated with high-grain feeding. 55,56 Consistently, we found that NDF degradation was suppressed to a greater extent by hyperosmolality than the degradation of NFCs was. The further increase in propionate percentage upon betaine addition under the hyperosmotic stress suggests that betaine is used by the dominating microbes to support fermentation.…”

Section: Discussionsupporting

confidence: 77%

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Betaine addition as a potent ruminal fermentation modulator under hyperthermal and hyperosmotic conditions in vitro

Mahmood

Petri

Gavrău³

et al. 2020

J Sci Food Agric

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BACKGROUND Climatic and dietary shifts predispose ruminal microbes to hyperthermal and hyperosmotic stress, leading to poor fermentation and subsequently adverse effects on ruminant productivity. Betaine may function as substrate, osmolyte, antioxidant, and methyl donor for microbes. However, its effect depends on the extent of microbial catabolism. This study revealed the ruminal disappearance kinetics of betaine and its dose effect on ruminal fermentation during thermal and osmotic stress using a rumen simulation technique. RESULTS Three different betaine doses were used: 0, 50, and 286 mg L−1; each was assigned to two incubation temperatures (39.5 and 42 °C) and two osmotic conditions (295 and 420 mOsmol kg−1). Betaine disappeared rapidly within the first 6 h of incubation; however, the rate was lower during hyperosmotic stress (P < 0.05), the stress condition that also suppressed the overall fermentation and degradation of organic nutrients and decreased the bacterial diversity (P < 0.001). During hyperosmotic stress, betaine shifted the fermentation pathway to more propionate (P < 0.05). Betaine counteracted the negative effect of hyperthermal stress on total short‐chain fatty acid concentration (P < 0.05) without affecting the composition. Both stress conditions shifted the bacterial composition, but the effect of betaine was minimal. CONCLUSION Despite its rapid ruminal disappearance, betaine modulated microbial fermentation in different ways depending on stress conditions, indicating the plasticity of the betaine effect in response to various kinds of physicochemical stress. Although betaine did not affect the abundance of ruminal microbiota, the enhanced fermentation suggests an improved microbial metabolic activity under stress conditions. © 2020 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 77%

Betaine addition as a potent ruminal fermentation modulator under hyperthermal and hyperosmotic conditions in vitro

Mahmood

Petri

Gavrău³

et al. 2020

J Sci Food Agric

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“…Such an elevated abundance of Parabacteroides may have been related to the relatively larger quantities of amino acids and monosaccharides observed in the rumen of the PWM-fed dairy calves. Moreover, feeding calves with UBM elevated the relative abundance in the rumen (both mucosa and digesta) of the genus Fibrobacter, which is responsible for the degradation of dietary fibre and polysaccharides, and this result was likely related to the lack of antibiotics in the UBM2223. Therefore, more volatile fatty acids and monosaccharides may have been produced in the rumen of dairy calves fed UBM.…”

Section: Discussionmentioning

confidence: 99%

“…At the genus level, 9 significantly different genera were detected among the dietary groups, and the results indicated that the abundances of the genera Prevotella, Parabacteroides, Faecalibacterium , and Mitsuokella were remarkably enhanced in the caecum (both mucosa and digesta) of the AWM calves compared with the other calves. The abundance of Prevotella in the caecum of the AWM calves may have been caused by the acid content and the concomitant low pH2224. A previous report indicated that the relative abundance of Prevotella in the caecum tended to increase linearly in rabbits that drank acidified drinking water29.…”

Section: Discussionmentioning

confidence: 99%

Influence of dairy by-product waste milk on the microbiomes of different gastrointestinal tract components in pre-weaned dairy calves

Deng

Wang

Zou

et al. 2017

Sci Rep

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The community structure of colonised bacteria in the gastrointestinal tracts (GITs) of pre-weaned calves is affected by extrinsic factors, such as the genetics and diet of the calves; however, the dietary impact is not fully understood and warrants further research. Our study revealed that a total of 6, 5, 2 and 10 bacterial genera showed biologically significant differences in the GITs of pre-weaned calves fed four waste-milk diets: acidified waste milk, pasteurised waste milk, untreated bulk milk, and untreated waste milk, respectively. Specifically, generic biomarkers were observed in the rumen (e.g., Bifidobacterium, Parabacteroides, Fibrobacter, Clostridium, etc.), caecum (e.g., Faecalibacterium, Oxalobacter, Odoribacter, etc.) and colon (e.g., Megamonas, Comamonas, Stenotrophomonas, etc.) but not in the faeces. In addition, the predicted metabolic pathways showed that the expression of genes related to metabolic diseases was increased in the calves fed untreated waste milk, which indicated that untreated waste milk is not a suitable liquid diet for pre-weaned calves. This is the first study to demonstrate how different types of waste milk fed to pre-weaned calves affect the community structure of colonised bacteria, and the results may provide insights for the intentional adjustment of diets and gastrointestinal bacterial communities.

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“…Lactic acidosis is mainly defined as an acute GIT disorder (Zein-Eldin et al, 2014) and is clinically characterized by depression, anorexia, inactivity, abdominal distension, diarrhea and general weakness (Ullah et al, 2013). Lactic acidosis occurs due to a disproportionate consumption of readily fermentable carbohydrates that decrease ruminal pH to improper levels and lead to the rapid production of lactic acid with rapid fermentation (Mickdam et al, 2016). Lactic acidosis is considered a dramatic form of ruminal microbial fermentative disorder and is lethal in less than 24 hours in some cases (Huo et al, 2014).…”

Section: -Herbal Medicine For the Treatment Of Rumen Function Insuffmentioning

confidence: 99%

A Review: Herbal Medicine as an Effective Therapeutic Approach for Treating Digestive Disorders in Small Ruminants

Zeineldin¹,

Abdelmegeid²,

Barakat³

et al. 2018

AJVS

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Key words:Gastrointestinal; herbal medicine; therapy; small ruminant.Gastrointestinal (GIT) disorder is a significant health and economic problem that account for extraordinary financial loss in small ruminants. Despite the great efforts in management, GIT disorder remains the main cause of animal mortality, weight gain retardation and production loss in the small ruminant industry. Traditionally, herbal medicine has been used to treat and prevent various ruminant disorders. Herbs have nutritional and pharmaceutical properties that interact with one another polyvalently to increase the depth and breadth of the clinical effects compared with those seen in conventional pharmacological drugs. Recently, there has been an increase in the utilization of herbal medicine, reflecting an expanding trust in such therapeutic approaches. These methods have the advantage of utilizing locally available plants with high medicinal properties. Additionally, medicinal plants have the advantages of availability, compatibility with the body, simplicity of storage and negligible side effects. Here, we review representative examples of research available on the most commonly used traditional herbal remedies for the treatment of the most common digestive disorders (diarrhea, rumen insufficiency and GIT nematodes) in small ruminants. We also provide an extensive background on the recent advancement in the adoption of modern platforms in this field and research possibilities for future drug discovery and development.

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Rumen microbial abundance and fermentation profile during severe subacute ruminal acidosis and its modulation by plant derived alkaloids in vitro

Cited by 53 publications

References 44 publications

Betaine addition as a potent ruminal fermentation modulator under hyperthermal and hyperosmotic conditions in vitro

Betaine addition as a potent ruminal fermentation modulator under hyperthermal and hyperosmotic conditions in vitro

Influence of dairy by-product waste milk on the microbiomes of different gastrointestinal tract components in pre-weaned dairy calves

A Review: Herbal Medicine as an Effective Therapeutic Approach for Treating Digestive Disorders in Small Ruminants

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