Ruminal methane production: Associated microorganisms and the potential of applying hydrogen-utilizing bacteria for mitigation

Lan, Wei; Yang, Chunlei

doi:10.1016/j.scitotenv.2018.11.180

Cited by 128 publications

(83 citation statements)

References 230 publications

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“…Archaea occupy many ecological niches in the ruminal ecosystem, and they primarily function to scavenge H 2 to keep the rumen milieu favourable for microbial fermentation [ 32 ]. Rumen archaea can be categorized as hydrogenotrophic, aceticlastic, or methylotrophic based on the preferred H 2 substrate of formate, acetate, and methylamines, respectively, the earlier being most abundant in the rumen [ 33 ]. This study focused on understanding the community diversity and population changes of archaea within either the liquid or solid rumen phase under controlled temperature and osmotic stress, with and without betaine supplementation.…”

Section: Discussionmentioning

confidence: 99%

Betaine Modulates Rumen Archaeal Community and Functioning during Heat and Osmotic Stress Conditions In Vitro

Mahmood

Khiaosa‐ard

Zebeli

et al. 2020

Archaea

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Rumen archaea play an important role in scavenging ruminal hydrogen (H2) and thus facilitate rumen fermentation. They require optimum temperature and osmolality for their growth and metabolism; however, a number of external factors may put archaea under heat and osmotic stress. Betaine is an osmolyte, molecular chaperone, and antioxidant; therefore, it bears potential to combat against these stressors. In this in vitro study, three betaine levels, namely, 0 (control), 51 (low), and 286 (high) ppm, were used. Each of these was subjected to two temperatures (39.5 and 42°C) and two osmolality conditions (295 and 420 mOsmol kg-1) with n = 6 per treatment. Sequencing analyses of the solid phase (which use solid materials containing primarily fibrous materials of low-density feed particles) and the liquid phase (rumen fermenter liquid) using 16S rRNA revealed that more than 99.8% of the ruminal archaea in fermenters belong to the phylum Euryarchaeota. At the genus level, Methanobrevibacter was the most prevalent in both phases, and Methanosaeta was only detected in the liquid phase. The genera Methanobrevibacter and Methanobacterium both showed a positive correlation with methane (CH4) formation in the liquid and solid phases, respectively ( P < 0.05 ). Heat stress increased the relative abundance of genus Methanimicrococcus at the expense of candidate archaeal genus Vadin CA11 ( P < 0.05 ). In the solid phase, osmotic stress significantly reduced the Shannon and Simpson indices of diversity, and relative abundance was higher for Methanobrevibacter at the expense of Methanimicrococcus. In the liquid phase, osmotic stress increased not only the abundance-based coverage estimator (ACE) and singles parameters of diversity but also the relative abundances of Methanosphaera and Methanobacterium. The overall decrease in all gas parameters and estimated metabolic hydrogen ([2H]) utilization was observed during osmotic stress conditions ( P < 0.05 ). Betaine enhanced the diversity of solid phase archaea as indicated by the increase in ACE and singles during heat stress, and only a high dose improved all diversity parameters in the liquid phase during osmotic stress ( P < 0.05 ). Thus, betaine alleviates the effects of heat stress and osmotic stress on the archaea community.

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Section: Discussionmentioning

confidence: 99%

Betaine Modulates Rumen Archaeal Community and Functioning during Heat and Osmotic Stress Conditions In Vitro

Mahmood

Khiaosa‐ard

Zebeli

et al. 2020

Archaea

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“…Ma et al (2016) reported that supplementation with garlic extraction improved nutrient utilization by stimulating cellulolytic bacterial activity in the rumen [30]. Bioactive components present in garlic skin are polyphenolic in nature [31] and induce a positive effect on energy metabolism [32]. Therefore, improved growth rates in the GAS group could be attributed to lower energy loss, and higher nutrient utilization.…”

Section: Discussionmentioning

confidence: 99%

Garlic Skin Induced Shifts in the Rumen Microbiome and Metabolome of Fattening Sheep

Zhu

et al. 2020

Preprint

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Background: Garlic and its constituents exhibit activities on modifying rumen fermentation and improving growth performance. As a by-product of garlic processing, garlic skin contains similar bioactive components as garlic bulb. However, studies in ruminants using garlic skin are scarce. This experiment was conducted to investigate the effects of garlic skin supplementation on rumen fermentation characterizes, growth performance, and involved mechanism in ruminants. Twelve Hu lambs were randomly assigned into one of two treatments: basal diet (CON) or basal diet supplemented with 80 g/kg DM of gallic skin (GAS). The experiment lasted for10 weeks, with the first 2 weeks for adaptation. Results: The results revealed that the average daily gain and volatile fatty acid concentration were higher (P < 0.05) in lambs fed GAS than that in the control group. Garlic skin supplementation did not significantly (P > 0.10) affect the α-diversity indices. Increased (P < 0.05) abundances of Prevotella, Bulleidia, Howardella, Methanosphaera but a decreased (P < 0.05) abundance of Fretibacterium were observed in GAS-fed lambs. Besides, the garlic skin supplementation favorably regulated (P < 0.05) pyrimidine metabolism, purine metabolism, vitamin B6 and B1 metabolism. Moreover, high correlations were observed between fluctuant rumen microbiota and metabolites. Conclusions: Supplementation of garlic skin improved the growth performance of sheep by modifying rumen fermentation through inducing shifts in the rumen microbiome and metabolome.

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“…Ma et al [19] reported that supplementation with garlic extraction improved nutrient utilization by stimulating cellulolytic bacterial activity in the rumen. Bioactive components present in garlic skin are polyphenolic in nature [20] and induce a positive effect on energy metabolism [21]. Therefore, improved growth rates in the GAS group could be attributed to lower energy loss, and higher nutrient utilization.…”

Section: Discussionmentioning

confidence: 99%

Garlic skin induced shifts in the rumen microbiome and metabolome of fattening sheep

Zhu

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Garlic and its constituents exhibit activities on modifying rumen fermentation and improving growth performance. As a by-product of garlic processing, garlic skin contains similar bioactive components as garlic bulb. However, studies in ruminants using garlic skin are scarce. This study was conducted to investigate the effects of garlic skin supplementation on rumen fermentation characterizes, growth performance, ruminal microbes and metabolites in ruminants. Twelve Hu lambs were randomly assigned into one of two treatments: basal diet (CON) or basal diet supplemented with 80 g/kg DM of gallic skin (GAS). The experiment lasted for10 weeks, with the first 2 weeks for adaptation. Results The results revealed that the average daily gain and volatile fatty acid concentration were higher (P < 0.05) in lambs fed GAS than that in the control group. Garlic skin supplementation did not significantly (P > 0.10) affect the α-diversity indices. Increased (P < 0.05) abundances of Prevotella, Bulleidia, Howardella, Methanosphaera but a decreased (P < 0.05) abundance of Fretibacterium were observed in GAS-fed lambs. In addition, the garlic skin supplementation favorably regulated (P < 0.05) pyrimidine metabolism, purine metabolism, vitamin B6 and B1 metabolism. Moreover, high correlations were observed between fluctuant rumen microbiota and metabolites. Conclusions Supplementation of garlic skin improved the growth performance of sheep by modifying rumen fermentation through inducing shifts in the rumen microbiome and metabolome.

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Ruminal methane production: Associated microorganisms and the potential of applying hydrogen-utilizing bacteria for mitigation

Cited by 128 publications

References 230 publications

Betaine Modulates Rumen Archaeal Community and Functioning during Heat and Osmotic Stress Conditions In Vitro

Betaine Modulates Rumen Archaeal Community and Functioning during Heat and Osmotic Stress Conditions In Vitro

Garlic Skin Induced Shifts in the Rumen Microbiome and Metabolome of Fattening Sheep

Garlic skin induced shifts in the rumen microbiome and metabolome of fattening sheep

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