Runx2 and Nell-1 in dental follicle progenitor cells regulate bone remodeling and tooth eruption

Zeng, Li; He, Hong; Sun, Mingjie; Gong, Xiangyang; Zhou, Min-Bo; Hong, Yaya; Wu, Yongjia; Chen, Xuepeng; Chen, Qianming

doi:10.1186/s13287-022-03140-3

Cited by 21 publications

(19 citation statements)

References 103 publications

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“…The impaired osteogenic potential of SHED from patients with CCD. have mainly focused on the mechanism of dental replacement (Qin et al, 2019;Xin et al, 2022;Zeng et al, 2022). However, the clinical features of severe caries and early tooth loss, which also influence the oral and mental health of individuals with CCD, have been received little attention.…”

Section: Discussionmentioning

confidence: 99%

“…As RUNX2 is the causative gene for CCD and is important in tooth formation, different types of cells with heterozygous mutations of RUNX2 show diverse dysfunction, leading to various oral manifestations when mutated. For instance, dental follicle cells with RUNX2 mutations show weaker osteogenesis and osteoclastic ability, resulting in tooth eruption and alveolar bone remodeling difficulties, and periodontal ligament cells with RUNX2 mutations demonstrate an affected balance between osteoblast and osteoclast activity, leading to tooth eruption difficulties and deciduous tooth retention (Komori et al, 1997;Sun et al, 2016;Zeng et al, 2022). In the present study, SHED obtained from the proband showed a weaker capacity for proliferation and osteogenesis than SHED obtained from healthy individuals (Figures 5, 6), consistent with previous reports.…”

Section: Discussionmentioning

confidence: 99%

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Amyloid-mediated remineralization for tooth hypoplasia of cleidocranial dysplasia

Guo

Yang²,

Liu³

et al. 2023

Front. Cell. Infect. Microbiol.

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IntroductionCleidocranial dysplasia (CCD) is an autosomal-dominant, heritable skeletal and dental disease, involving hypoplastic clavicles, defective ossification of the anterior fontanelle, dentin and enamel hypoplasia, and supernumerary teeth, which can seriously affect the oral and mental health of patients. Amyloid-like protein aggregation, which is established by lysozyme conjugated with polyethylene glycol (Lyso-PEG), forms a mineralized nanofilm layer on a healthy enamel surface. However, whether it can form a remineralization layer in dental tissues from CCD remains unclear.MethodsThis study evaluated deciduous teeth from healthy individuals and a patient with CCD. Because pulp and dentin are functionally closely related, stem cells from human exfoliated deciduous teeth (SHED) from CCD patients and healthy individuals were collected to compare their biological properties.ResultsThe results found that deciduous teeth from patients with CCD exhibited dentin hypoplasia. In addition, the proliferative ability and osteogenic potential of SHED from patients with CCD were lower than those of control individuals. Finally, Lyso-PEG was applied to dentin from the CCD and control groups, showing a similar remineralization-induced effect on the dentin surfaces of the two groups.ConclusionThese results extend our understanding of the dentin and SHED of patients with CCD, exhibiting good caries-preventive capacity and good biocompatibility of Lyso-PEG, thus providing a novel dental therapy for CCD and patients with tooth hypoplasia.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Amyloid-mediated remineralization for tooth hypoplasia of cleidocranial dysplasia

Guo

Yang²,

Liu³

et al. 2023

Front. Cell. Infect. Microbiol.

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“…Second, there may have been an immunosuppressed population of cells in both tissues. Studies have shown that cytokines secreted by dental follicle cells inhibited lymphocyte proliferation ( 36 ), and osteoprotegerin secreted by dental follicle cells inhibited monocyte recruitment and osteoclast formation ( 37 ). Despite the need to form an osteoclastic resorption channel during tooth germ development and eruption, this seems consistent given the simultaneous alveolar bone formation.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome landscape comparison of periodontium in developmental and renewal stages

Deng

Luo²,

Xie³

et al. 2023

Front. Endocrinol.

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ObjectivesPeriodontium regeneration remains a significant challenge in clinics and research, and it is essential to understand the stage-specific biological process in situ. However, differing findings have been reported, and the mechanism has yet to be elucidated. The periodontium of adult mice molars is considered to be stable remodeling tissue. At the same time, the continuously growing incisors and the developing dental follicle (DF) of postnatal mice highly represent fast remodeling tissue. In this study, we attempted to explore different clues of temporal and spatial comparisons to provide improved references for periodontal regeneration.MethodsPeriodontal tissues from the developing periodontium (DeP) of postnatal mice, and continuously growing periodontium (CgP) and stable remodeling periodontium (ReP) of adult mice were isolated and compared using RNA sequencing. Based on the Dep and CgP separately compared with the ReP, differentially expressed genes and signaling pathways were analyzed using GO, KEGG databases, and Ingenuity Pathway Analysis (IPA). The results and validation were obtained by immunofluorescence staining and RT-PCR assays. Data were expressed as means ± standard deviation (SD) and analyzed by GraphPad Prism 8 software package, and one-way ANOVA was used to test multiple groups.ResultsPrincipal component analysis showed that the three groups of periodontal tissue were successfully isolated and had distinct expression profiles. A total of 792 and 612 DEGs were identified in the DeP and CgP groups compared with the ReP. Upregulated DEGs in the DeP were closely related to developmental processes, while the CgP showed significantly enhanced cellular energy metabolism. The DeP and CgP showed a common downregulation of the immune response, with activation, migration, and recruitment of immune cells. IPA and further validation jointly suggested that the MyD88/p38 MAPK pathway played an essential regulatory role in periodontium remodeling.ConclusionTissue development, energy metabolism, and immune response were critical regulatory processes during periodontal remodeling. Developmental and adult stages of periodontal remodeling showed different expression patterns. These results contribute to a deeper understanding of periodontal development and remodeling and may provide references for periodontal regeneration.

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“…76 This protein is essential for osteoblasts and osteoclasts differentiation. 77 Some research also mentions that upregulated Runx2 and Sox6 also contribute to bone formation, especially for chondrocyte differentiation. 78 Related to increased runx2 expression after supplementation of GSE on the arthritis model, 53 the supplementation also decreased the HIF-1α expression in the alveolar bone.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Grape Seed Extract on the Alveolar, Jaw, and Skeletal Bone Remodeling: A Scoping Review

et al. 2023

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Herbal medicine has an important part in promoting and maintaining human health. One of them was grape seed extract (GSE). Various potentials of GSE in human health have been explored, and its potential for maintaining bone health is promising. Some initial research has provided evidence that the GSE was able to affect bone remodeling (bone resorption and bone formation). This scoping review analyzed and discussed all the reports on the effect of GSE on bone healing and bone remodeling in animals in the alveolar bone, jaw bone, and skeletal bone. The further purpose is to give an opportunity to research and development of supplementation of GSE for humans.The Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) 2020 guidelines were used to compose this scoping review through database on Scopus, PubMed, Science Direct, Web of Science, Embase, and manual search until December 2022. The inclusion criteria were a study that analyzed the effect of supplementation GSE on all bones.All included study was in vivo study with supplementation of GSE. The supplementation of GSE affects the alveolar bone, jaw bones, and skeletal bone by promoting bone formation and inhibiting bone resorption by suppressing inflammation, apoptosis pathways, and osteoclastogenesis. It not only supports bone remodeling in bone inflammation, osteonecrosis, osteoporosis, and arthritis but also the GSE increases bone health by increasing the density and mineral deposition in trabecula and cortical bone.The supplementation of GSE supports bone remodeling by interfering with the inflammation process and bone formation not only by preventing bone resorption but also by maintaining bone density.

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Runx2 and Nell-1 in dental follicle progenitor cells regulate bone remodeling and tooth eruption

Cited by 21 publications

References 103 publications

Amyloid-mediated remineralization for tooth hypoplasia of cleidocranial dysplasia

Amyloid-mediated remineralization for tooth hypoplasia of cleidocranial dysplasia

Transcriptome landscape comparison of periodontium in developmental and renewal stages

The Effect of Grape Seed Extract on the Alveolar, Jaw, and Skeletal Bone Remodeling: A Scoping Review

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