Rural–urban focus of canine visceral leishmaniosis in the far western region of Santa Catarina State, Brazil

Maziero, Nelí; Soccol, Vanete Thomaz; Steindel, Mário; Link, Juliana Seger; Rossini, Diego; Alban, Silvana Maria; Nascimento, Aguinaldo José do

doi:10.1016/j.vetpar.2014.06.005

Cited by 11 publications

(16 citation statements)

References 21 publications

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“…The DPP  test was performed using serum samples according to the manufacturer's protocol (Bio-Manguinhos/Fiocruz, Rio de Janeiro, BR). The ELISA test was standardized according to Maziero et al (MAZIERO et al, 2014). The cut-off point was determined based on the average absorbance of four negative sera (dogs born in Curitiba -Paraná) plus three standard deviations, repeated on each plate.…”

Section: Serodiagnosismentioning

confidence: 99%

Variables associated with the prevalence of anti-Leishmania spp. antibodies in dogs on the tri-border of Foz do Iguaçu, Paraná, Brazil

Dias

Soccol

Pasquali

et al. 2018

Rev. Bras. Parasitol. Vet.

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The aim of this study was to investigate the occurrence of anti-Leishmania spp. antibodies in dogs from localities in the city of Foz do Iguaçu, Paraná state, Brazil, on the border with Argentina and Paraguay. Blood samples dogs were collected to perform the following serologic tests: immunochromatographic DPP® rapid test, indirect immunoenzymatic assay (ELISA) and indirect immunofluorescence assay (IFA). In 2012, 285 dogs were analyzed on Argentina border, and in 2013, serum samples from 396 dogs on the border of Paraguay were collected. Using ELISA for screening and IFA for the confirmatory test, the results showed that the antibody prevalence was 1.8% (5/285) on the border of Argentina and 3.0% (12/396) on Paraguay border. When using the DPP® for screening and ELISA as a confirmatory analysis, we observed a seroreagent prevalence in dogs of 2.5% (7/285) on Argentina border and 5.1% (20/396) on Paraguay border. The non-public collection of domestic waste (p= 0.0004) was shown to be associated with leishmaniasis. This study shows the presence of leishmaniasis and suggest the emergence of canine visceral leishmaniasis in state of Paraná due to the confirmed occurrence of seroreactive dogs on Argentina and Paraguay border, which has environmental and geographical characteristics that favor the spread of the parasite.

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Section: Serodiagnosismentioning

confidence: 99%

Variables associated with the prevalence of anti-Leishmania spp. antibodies in dogs on the tri-border of Foz do Iguaçu, Paraná, Brazil

Dias

Soccol

Pasquali

et al. 2018

Rev. Bras. Parasitol. Vet.

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“…infantum in the South Region of Brazil is represented by a new cluster in western Santa Catarina State. The first autochthonous cases of cVL in this state were in Florianópolis in 2010 and in the western region in 2013 (São Miguel do Oeste and Descanso) [82]. These L .…”

Section: Discussionmentioning

confidence: 99%

“…2006 to 2010: first VL cases registered Argentina and in the state of Rio Grande do Sul, and dispersion from cities in Paraguay (e.g. [9,38,80–82]); 5. 2011 to 2018: Dispersion of VL cases in South Brazil (e.g.…”

Section: Methodsmentioning

confidence: 99%

Dispersion of Leishmania (Leishmania) infantum in central-southern Brazil: Evidence from an integrative approach

et al. 2019

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Leishmania (Leishmania) infantum is the zoonotic agent of visceral leishmaniasis (VL), a disease with a global distribution. The transmission scenario of VL has been undergoing changes worldwide, with the biologic cycle invading urbanized areas and dispersing the parasites into other previously free areas. The epidemiological cycle in Brazil has dispersed from the Northeast to other regions of the country. In this study, an integrative approach, including genotyping Brazilian strains of L . (L . ) infantum for 14 microsatellite markers and reviewing historical records of the disease, was used to assess dispersion routes throughout central-southern Brazil. Our results support three L . (L . ) infantum dispersion routes: A) dispersion from Bolivia to the states of Mato Grosso, Mato Grosso do Sul and São Paulo via the Bolivia-Brazil gas pipeline from 1998 to 2005; B) VL dispersion from Paraguay to the Brazilian side of the triple border (Foz do Iguaçu and Santa Terezinha de Itaipu) during after 2012; and C) emergence of a new L . (L . ) infantum cluster in western Santa Catarina State and its dispersion to southern Paraná State (municipality of Pato Branco), after 2013. Hypotheses regarding possible entries of Leishmania (L . ) infantum into the area of the triple border are presented and discussed. Understanding how VL has dispersed is vital to the development of control measures for this disease and to avoid future dispersion events.

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“…Two serological tests were used in the serological survey: an immunoassay test (using crude antigen cELISA), as proposed by Mazieiro et al, 2014 [ 10 ], and the Dual Path Platform (DPP® cVL, Biomanguinhos).…”

Section: Methodsmentioning

confidence: 99%

“…The first autochthonous cVL (canine Visceral Leishmaniasis) cases in this region were reported in 2009 in São Borja, state of Rio Grande do Sul [ 8 ]. In the state of Santa Catarina, the first autochthonous cases of cVL were reported in 2010, in the city of Florianopolis [ 9 ], and in 2013 in the western region (São Miguel do Oeste and Descanso), [ 10 ]. Concomitantly, VL has expanded its reach to neighbouring countries (Argentina and Paraguay), where a gradual increase in human deaths due to it has been observed.…”

Section: Introductionmentioning

confidence: 99%

More than the eyes can see: The worrying scenario of canine leishmaniasis in the Brazilian side of the triple border

Soccol

Pasquali

Pozzolo³

et al. 2017

PLoS ONE

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A cross-sectional epidemiological study in the extreme-west of the state of Paraná was carried out to access the prevalence, distribution, and risk variables of canine Visceral Leishmaniases (cVL). This study was conducted in three areas, two cities of far west of Parana state: Foz do Iguaçu (FI) and Santa Terezinha de Itaipu (STI), and along two transects between these two municipalities. To sample the entire urban area, the cities (FI and STI) were divided into a grid of squares of 400 m2 (patch). Among the 526 patches, 123 in FI, 40 in the transects and 33 in STI were selected according to the ‘worst scenario’ criterion. In the transect areas, in each 0.86 km five dogs from houses were surveyed to leishmaniasis. In each patch, blood of five dogs from houses (and from neighborhood when necessary) in the areas that seemed to be the most appropriate for the proliferation of vector were surveyed. The infection of the dogs by cVL were assessed using two serological tests were used (cELISA and TR-DPP®), and, for those seropositive for both methods, the PCR method were used. Moreover, dogs presenting clinical signs or cutaneous lesions were sampled to PCR. The identification of Leishmania species was confirmed using PCR-RFLP followed by DNA sequencing. Micro, meso and macro scale environmental variables were also surveyed and statistically analyzed. The prevalence rate Leishmania infantum was 23.8% in FI, 4.7% in STI and 9.1% in the transects areas. Among the extrinsic variables analysed, the number of vectors and the presence of infected dogs in neighbouring were positively correlated with the occurrence of infected dogs. Dog size was positively correlated with cVL infection, while the quality of the dog’s nutrition affected cVL negatively. As for cutaneous leishmaniasis (CL), the first registry of dogs infected with L. braziliensis in the region shows that there is potential for transmission in peri-urban areas, since environmental conditions allow the proliferation of vectors capable of transmitting this species of parasite. cVL is widely spread in FI, with high prevalence. This supports the hypothesis that the parasite has been present in the region for longer than previously believed, despite the fact that the presence of leishmaniais in the region has only been recognized recently. It is important to control the population of dogs infected with L. infantum (parasite and non-antibodies) to prevent the spread of the disease to other dogs and also to people in the region.

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Rural–urban focus of canine visceral leishmaniosis in the far western region of Santa Catarina State, Brazil

Cited by 11 publications

References 21 publications

Variables associated with the prevalence of anti-Leishmania spp. antibodies in dogs on the tri-border of Foz do Iguaçu, Paraná, Brazil

Variables associated with the prevalence of anti-Leishmania spp. antibodies in dogs on the tri-border of Foz do Iguaçu, Paraná, Brazil

Dispersion of Leishmania (Leishmania) infantum in central-southern Brazil: Evidence from an integrative approach

More than the eyes can see: The worrying scenario of canine leishmaniasis in the Brazilian side of the triple border

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