Ruscogenin Ameliorated Sjögren’s Syndrome by Inhibiting NLRP3 Inflammasome Activation

He, J. P.; Wang, Yue; Xu, Lei; Xu, Changshui; Zhu, Yamei; Xu, Meimei; Chen, Yueyue; Guo, Liang; Hu, Wei; Xu, Dake; Jing, Rongyue

doi:10.1155/2022/6425121

Cited by 7 publications

(10 citation statements)

References 44 publications

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“…Ruscogenin, as a natural compound, has been previously reported to suppress NLRP3 inflammasome activation in autoimmune disease and ischemic injury (Cao et al, 2016, He et al, 2022. In our study, we found that Ruscogenin effectively inhibited E protein-induced pyroptosis via downregulating NLRP3 expression, and this effect may be linked to NF-κB inhibition.…”

Section: Discussionsupporting

confidence: 65%

“…Ruscogenin, as a natural compound, has been reported to suppress NLRP3 inflammasome activation in autoimmune disease and ischemic injury (Cao et al, 2016, He et al, 2022. To investigate whether Ruscogenin could alleviate E protein-induced THP-1 pyroptosis, THP-1 was pre-treated with various concentrations of Ruscogenin for 1 h followed by stimulation with 1 μg/ml of E protein.…”

Section: Ruscogenin Reversed Sars-cov-2 E Protein-induced Pyroptosismentioning

confidence: 99%

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SARS-CoV-2 E protein-induced THP-1 pyroptosis is reversed by Ruscogenin

Huang

Zha

et al. 2023

Biochem. Cell Biol.

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), an emerging pathogenic coronavirus, has been reported to cause excessive inflammation and dysfunction in multiple cells and organs, but the underlying mechanisms remain largely unknown. Here we showed exogenous addition of SARS-CoV-2 envelop protein (E protein) potently induced cell death in cultured cell lines, including THP-1 monocytic leukemia cells, endothelial cells and bronchial epithelial cells, in a time- and concentration-dependent manner. SARS-CoV-2 E protein caused pyroptosis-like cell death in THP-1 and led to GSDMD cleavage. In addition, SARS-CoV-2 E protein up-regulated the expression of multiple pro-inflammatory cytokines that may be attributed to activation of NF-κB, JNK and p38 signal pathways. Notably, we identified a natural compound, Ruscogenin, effectively reversed E protein-induced THP-1 death via inhibition of NLRP3 activation and GSDMD cleavage. In conclusion, these findings suggested that Ruscogenin may have beneficial effects on preventing SARS-CoV-2 E protein-induced cell death and might be a promising treatment for the complications of COVID-19.

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Section: Discussionsupporting

confidence: 65%

Section: Ruscogenin Reversed Sars-cov-2 E Protein-induced Pyroptosismentioning

confidence: 99%

SARS-CoV-2 E protein-induced THP-1 pyroptosis is reversed by Ruscogenin

Huang

Zha

et al. 2023

Biochem. Cell Biol.

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“…8-10 NLRP3 overexpression could reverse the up-regulation of pro-apoptotic factor (Bax) and the downregulation of anti-apoptotic factor (Bcl-2) in acinar cells. 28 Anfibatide could inhibit the expression of NLRP3, ASC, cleaved-caspase-1, Bax and cleaved-caspase-3, promoted the expression of Bcl-2 in cerebral ischemia reperfusion in vivo and in vitro, which indicates that anfibatide could alleviate inflammation and apoptosis through inhibiting NF-kappaB/NLRP3 axis. 29 Alpha-mangostin exerted a protective effect on NLRP3 inflammasome-mediated apoptosis in LPS-induced NPCs through regulating NF-κB signaling.…”

Section: Discussionmentioning

confidence: 87%

“…The NLRP3 inflammasome can be activated by many activators, such as reactive oxygen species (ROS), K + channel openers, uric acid, and endotoxins, and plays an important role in insulin resistance and the pathogenesis of diabetes 8–10 . NLRP3 overexpression could reverse the up‐regulation of pro‐apoptotic factor (Bax) and the downregulation of anti‐apoptotic factor (Bcl‐2) in acinar cells 28 . Anfibatide could inhibit the expression of NLRP3, ASC, cleaved‐caspase‐1, Bax and cleaved‐caspase‐3, promoted the expression of Bcl‐2 in cerebral ischemia reperfusion in vivo and in vitro, which indicates that anfibatide could alleviate inflammation and apoptosis through inhibiting NF‐kappaB/NLRP3 axis 29 .…”

Section: Discussionmentioning

confidence: 99%

Irisin inhibits NLRP3 inflammasome activation in HG/HF incubated cardiac microvascular endothelial cells with H/R injury

Xin¹,

Zhang

Hao³

et al. 2022

Microcirculation

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Purpose: NLRP3 inflammasome mediates myocardial ischemia/reperfusion (MI/R) injury and diabetic vascular endothelia dysfunction. However, the role of NLRP3 inflammasome in MI/R injury with diabetes has not been fully described. Irisin plays an important role in anti-inflammation and improves endothelial function in type 2 diabetes. The current study aimed to investigate the effect of irisin on regulating NLRP3 inflammasome activation in diabetic vascular endothelia dysfunction. Methods: Cardiac microvascular endothelial cells (CMECs) were cultured and subjected to high glucose/high fat (HG/HF) receiving hypoxia/reoxygenation (H/R) with irisin incubation or not. Then, apoptosis, viability, migration, NO secretion, and inflammasome activation were examined. Results: The hypoxic CMECs exhibited increased apoptosis, impaired viability, and migration, even decreased NO secretion and enhanced inflammasome activation. Moreover, irisin incubation decreased NLRP3 activation and attenuated cell injury in HG/HF cultured CMECs subjected to H/R injury, which was abolished by NLRP3 inflammasome activation. Meanwhile, NLRP3 inflammasome siRNA also attenuated H/R injury in CMECs under HG/HF condition. Conclusion:The current study demonstrated for the first time that irisin inhibits NLRP3 inflammasome activation in CMECs as a novel mechanism in myocardial ischemia/reperfusion injury in diabetes.

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“…In addition to its use in the treatment of hemorrhoids, this substances has also been reported to have bene cial effects in the treatment of acute and chronic in ammation, prevention of blood-brain barrier dysfunction and alleviation of acute lung injury, and pulmonary arterial hypertension diseases [7]. In addition to these, it suppresses hepatocellular carcinoma metastasis, alleviates lipopolysaccharide-induced pulmonary endothelial cell apoptosis, improves Sjögren's syndrome, and alleviates particulate matter-induced acute lung injury [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Ruscogenin, Neoruscogenin, Trimebutin, and Parabens in Cream Formulation by Reverse Phase High Performance Liquid Chromatography

Özen,

Nemutlu

2023

Chromatographia

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A simple and rapid reverse phase high performance liquid chromatography (RP-HPLC) method for analysis of ruscogenin (RUS), neoruscogenin (NRUS), trimebutine (TB), methylparaben (MP) propylparaben (PP) in pharmaceutical preparations has been developed and validated. In this study, a RP-HPLC method was developed for the determination of RUS, NRUS, TB, MP and PP and applied for analysis of pharmaceutical cream formulations. ACE C18 Column 121-2546 (250x4.6 mm) was used at 25 °C and gradient elution was performed with mobile phase consisting of a mixture of ACN:Buffer (pH:3.9). RUS, NRUS, TB, MP and PP eluted within 17 minutes. Peak homogeneity data of RUS, NRUS, TB, MP and PP in the pharmaceutical cream samples peaks obtained using photodiode array detector, in the cream sample chromatograms, demonstrated the speci city of the method. The developed method was validated according to the ICH guidelines. Linear ranges were 1.00, 5.00, 10.0, 25.0, 50.0, 100, 150 μg/mL for MP, RUS and 5.00 -10.0, 25.0, 50.0, 100, 150, 200 μg/mL for NRUS, PP and 10.0-25.0, 50.0, 100, 150, 200 μg/mL for TB. Validation parameters, such as stability, linearity, sensitivity, accuracy, precision, recovery, robustness and ruggedness were evaluated according to ICH guidelines and the method was simple, rapid, selective, sensitive, accurate, precise, robust and rugged. We developed a fast, low-cost RP-HPLC method with time of 17 minutes for simultaneous analysis of RUS, NRUS, TB and preservatives (MP and PP) in pharmaceutical preparations with semi-solid dosage form. The developed method was successfully applied for the quanti cation of RUS, NRUS, TB and preservatives (MP and PP) in pharmaceutical preparations with semi-solid dosage form. The detection limit of the method was 0.07, 0.28, 0.07, 0.45, 0.02 µg mL -1 respectively for MP, TB, PP, NRUS and RUS. HighlightsThis study is the rst method for the simultaneous analysis of ruscogenin, neoruscogenin, trimebutine, propylparaben, and methylparaben.The developed RP-HPLC method is a reliable and stability-indicating for active pharmaceutical ingredients and preservatives.Validation studies were performed according to ICH guide.The developed method is simple, selective, and sensitive.

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Ruscogenin Ameliorated Sjögren’s Syndrome by Inhibiting NLRP3 Inflammasome Activation

Cited by 7 publications

References 44 publications

SARS-CoV-2 E protein-induced THP-1 pyroptosis is reversed by Ruscogenin

SARS-CoV-2 E protein-induced THP-1 pyroptosis is reversed by Ruscogenin

Irisin inhibits NLRP3 inflammasome activation in HG/HF incubated cardiac microvascular endothelial cells with H/R injury

Simultaneous Determination of Ruscogenin, Neoruscogenin, Trimebutin, and Parabens in Cream Formulation by Reverse Phase High Performance Liquid Chromatography

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