Ruthenium(II) complexes targeting membrane as biofilm disruptors and resistance breakers in Staphylococcus aureus bacteria

Wang, Liqiang; Liu, Lianghong; Wang, Xuerong; Tan, Yanhui; Duan, Xuemin; Cheng, Jianxin; Xiong, Yan‐Shi; Jiang, Guijuan; Wang, Jintao; Liao, Xiangwen

doi:10.1016/j.ejmech.2022.114485

Cited by 23 publications

(22 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ru- 1 , with the strongest lipophilicity, showed the best antibacterial activity, which was obviously due to the dtb ligands containing tert -butyl groups. It was also reported that Ru( ii ) polypyridine complexes with dtb auxiliary ligands showed the best antibacterial activity with a MIC value of 12.5 μg mL −1 against S. aureus , 32 which was consistent with the result here.…”

Section: Resultssupporting

confidence: 90%

“…34,35 Nevertheless, Ru- 1 exhibited more effective antibacterial activity against S. aureus than all of our previously reported Ru( ii ) complexes. 21,22,32 To further explore the antibacterial activity, the minimum bactericidal concentration (MBC) of the three compounds was determined. As shown in Table 1, the most effective complex Ru- 1 exhibited an MBC value of only 2 μg mL −1 .…”

Section: Resultsmentioning

confidence: 99%

“…According to previous studies, this may be related to the mechanism that ruthenium complexes could damage membranes. 32 In addition, it was also reported that vancomycin could act by inhibition of lipid II binding/peptidoglycan synthesis supplemented by membrane damage. 36 Therefore, Ru- 1 's ability to kill bacteria quickly may be related to the destruction of bacterial cell membranes.…”

Section: Resultsmentioning

confidence: 99%

“…After 30 min, the wax worms were treated with 5 μL 100% DMSO, 10 mg kg −1 and 20 mg kg −1 Ru- 1 or 20 mg kg −1 vancomycin. Finally, the percent survival of G. mellonella wax worms was recorded every 24 h. 32…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Multi-target antibacterial mechanism of ruthenium polypyridine complexes with anthraquinone groups againstStaphylococcus aureus

Jiang

Chen

et al. 2023

RSC Med. Chem.

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Multi-target antibacterial mechanism of ruthenium polypyridine complexes with anthraquinone groups againstStaphylococcus aureus

Jiang

Chen

et al. 2023

RSC Med. Chem.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Resistance development study was performed as reported. [31] Firstly, the MIC values of Ru3 and Ampicillin were determined by serial 2-fold dilutions. [32] Specifically, to a fresh Tryptic Soy Broth medium containing ~10 5 bacteria per 1 mL, serial 2-fold dilutions of each drug (64 μM ~0.25 μM) were added.…”

Section: Chemmedchemmentioning

confidence: 99%

Ru(II) Complexes with Enaminone Structures for Rapid Sterilization of Staphylococcus aureus and MRSA with Little Accumulation of Drug Resistance

et al. 2023

View full text Add to dashboard Cite

Drug-resistant bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), pose a serious threat to human life. Therefore, there is urgent need to develop antibiotics with new chemical structures and antibacterial mechanisms, especially those that elicit little drug resistance after long-term use. Herein we synthesized three novel ruthenium complexes (Ru1À Ru3) containing the enaminone structures for the first time. At a concentration of 5 μM, Ru1À Ru3 can lead to a CFU reduction of about 5 log units towards S. aureus and MRSA. Interestingly, Ru3 displayed rapid bactericidal effects and could decrease the CFU numbers of both pathogens by 5 log units within 40 min. The control compounds (Ru4 and Ru5) without the enaminone structures displayed very poor antibacterial activity under the same conditions. Moreover, S. aureus did not show apparent drug resistance towards Ru3 after 20 passages incubation with a sublethal concentration. These results highlight the critical role of enaminone structures for antibacterial applications.

show abstract

Ruthenium polypyridine complexes containing prenyl groups as antibacterial agents against Staphylococcus aureus through a membrane‐disruption mechanism

Wang

Zhou

Chen

et al. 2023

Archiv der Pharmazie

Self Cite

View full text Add to dashboard Cite

Four new ruthenium polypyridyl complexes with prenyl groups, [Ru(bpy)2(MHIP)](PF6)2 (Ru(II)‐1), [Ru(dtb)2(MHIP)](PF6)2 (Ru(II)‐2), [Ru(dmb)2(MHIP)](PF6)2 (Ru(II)‐3), and [Ru(dmob)2(MHIP)](PF6)2 (Ru(II)‐4) (bpy = 2,2′‐bipyridine, dtb = 4,4′‐di‐tert‐butyl‐2,2′‐bipyridine, dmb = 4,4′‐dimethyl‐2,2′‐bipyridine, dmob = 4,4′‐dimethoxy‐2,2′‐bipyridine, and MHIP = 2‐(2,6‐dimethylhepta‐1,5‐dien‐1‐yl)−1H‐imidazo[4,f][1,10]phenanthroline), were synthesized and characterized. Their antibacterial activities against Staphylococcus aureus were assessed, and the minimum inhibition concentration (MIC) value of Ru(II)‐2 against S. aureus was only 0.5 µg/mL, showing the best antibacterial activity among them. S. aureus could be quickly killed by Ru(II)‐2 in 30 min and Ru(II)‐2 displayed an obvious inhibitive effect on the formation of a biofilm, which was essential to avoid the development of drug‐resistance. Meanwhile, Ru(II)‐2 exhibited a stable MIC value against antibiotic‐resistant bacteria. The antibacterial mechanism of Ru(II)‐2 was probably related to depolarization of the cell membrane, and a change of permeability was associated with the formation of reactive oxygen species, leading to leakage of nucleic acid and bacterial death. Furthermore, Ru(II)‐2 hardly showed toxicity to mammalian cells and the Galleria mellonella worm. Finally, murine infection studies also illustrated that Ru(II)‐2 was highly effective against S. aureus in vivo.

show abstract

Ruthenium(II) complexes targeting membrane as biofilm disruptors and resistance breakers in Staphylococcus aureus bacteria

Cited by 23 publications

References 32 publications

Multi-target antibacterial mechanism of ruthenium polypyridine complexes with anthraquinone groups againstStaphylococcus aureus

Multi-target antibacterial mechanism of ruthenium polypyridine complexes with anthraquinone groups againstStaphylococcus aureus

Ru(II) Complexes with Enaminone Structures for Rapid Sterilization of Staphylococcus aureus and MRSA with Little Accumulation of Drug Resistance

Ruthenium polypyridine complexes containing prenyl groups as antibacterial agents against Staphylococcus aureus through a membrane‐disruption mechanism

Contact Info

Product

Resources

About