Ruthenium metallodendrimers with anticancer potential in an acute promyelocytic leukemia cell line (HL60)

Michlewska, Sylwia; Йонов, Максим; Shcharbin, Dzmitry; Maroto-Díaz, Marta; Ramirez, Rafael Gomez; Mata, F. Javier de la; Bryszewska, Maria

doi:10.1016/j.eurpolymj.2016.12.011

Cited by 39 publications

(44 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such a dendrimer of generation 3 probably has a more flexible structure with better accessibility of internal groups to cellular content. It should be noted that non-generation dependent cytotoxicity occurred with some other dendrimeric structures [38,39].…”

Section: Discussionmentioning

confidence: 99%

Multi-Target Inhibition of Cancer Cell Growth by SiRNA Cocktails and 5-Fluorouracil Using Effective Piperidine-Terminated Phosphorus Dendrimers

Ihnatsyeu-Kachan

Dzmitruk

Apartsin

et al. 2017

Colloids and Interfaces

Self Cite

View full text Add to dashboard Cite

Currently, RNAi based approaches for cancer treatment involving short double stranded RNA molecules (siRNA) are under vigorous scrutinization. Due to numerous biological obstacles, siRNA delivery into target cells requires protective escort. On the other hand, combining of siRNA-mediated gene silencing and action of conventional chemotherapeutics can propose additional enhancement of anticancer activity. In the present study, we investigated a siRNA cocktail able to downregulate anti-apoptotic genes (BCL-xL, BCL-2, MCL-1) and the chemotherapeutic agent 5-fluorouracil (5-FU) to evaluate multi-target cytotoxic effect on human cervical carcinoma cells (HeLa cell line). Novel phosphorus containing dendrimers of 3rd and 4th generations (namely AE2G3 and AE2G4) with voluminous piperidine terminal cationic groups were designed and tested as siRNA carriers. Dendrimers of both generations showed remarkable ability to bind pro-apoptotic siRNAs and provided 80-100% siRNA uptake by HeLa cells in the serum containing medium, while the widespread transfection agent Lipofectamine showed only~40% uptake. SiRNA cocktail (in low concentrations 50 and 100 nM) delivered by AE2G3 dendrimer caused almost complete elimination of cancer cells. We have discovered considerable increase of 5-FU cytotoxic effect by addition of AE2G3/siRNA cocktail complexes in low doses. Thus, we demonstrated the effectiveness of combined multi-target siRNA anticancer approach and described new highly effective serum stable nanomaterial vehicle for gene-based drugs.

show abstract

Section: Discussionmentioning

confidence: 99%

Multi-Target Inhibition of Cancer Cell Growth by SiRNA Cocktails and 5-Fluorouracil Using Effective Piperidine-Terminated Phosphorus Dendrimers

Ihnatsyeu-Kachan

Dzmitruk

Apartsin

et al. 2017

Colloids and Interfaces

Self Cite

View full text Add to dashboard Cite

show abstract

“…Ru(II) metallodendrimers containing arene fragments were evaluated as an antitumor nanotherapy against resistant prostate cancer. 187,188 Promising cytotoxic, antiproliferative and antimetastatic behavior was observed. In particular, aer treatment with these nanocompounds, the tumor volume of the mouse was signicantly reduced compared with untreated animals.…”

Section: Metallodendrimersmentioning

confidence: 99%

Recent advances in metallopolymer-based drug delivery systems

et al. 2019

View full text Add to dashboard Cite

show abstract

“… IC 50 values (in µM) of some metallodendrimers reported in the literature. (a) [ 68 ]; (b) [ 71 ]; (c) [ 67 ]; (d) [ 69 ]; (e) [ 72 ]; (f) [ 77 ]; (g) [ 78 ] and (h) [ 79 ]. …”

Section: Figure A1mentioning

confidence: 99%

“…The incorporation of metal complexes in dendritic scaffolds, thus originating metallodendrimers, can increase the activity and selectivity of drugs based on transition metals [ 64 ]. Indeed, metallodendrimers can combine the anticancer potential of metal complexes with the features of the dendrimers as nanocarriers, and were described as having promising cytotoxicity against different cancer cell lines [ 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 ].…”

Section: Introductionmentioning

confidence: 99%

Poly(alkylidenimine) Dendrimers Functionalized with the Organometallic Moiety [Ru(η5-C5H5)(PPh3)2]+ as Promising Drugs Against Cisplatin-Resistant Cancer Cells and Human Mesenchymal Stem Cells

et al. 2018

View full text Add to dashboard Cite

Here and for the first time, we show that the organometallic compound [Ru(η5-C5H5)(PPh3)2Cl] (RuCp) has potential to be used as a metallodrug in anticancer therapy, and further present a new approach for the cellular delivery of the [Ru(η5-C5H5)(PPh3)2]+ fragment via coordination on the periphery of low-generation poly(alkylidenimine) dendrimers through nitrile terminal groups. Importantly, both the RuCp and the dendrimers functionalized with [Ru(η5-C5H5)(PPh3)2]+ fragments present remarkable toxicity towards a wide set of cancer cells (Caco-2, MCF-7, CAL-72, and A2780 cells), including cisplatin-resistant human ovarian carcinoma cell lines (A2780cisR cells). Also, RuCp and the prepared metallodendrimers are active against human mesenchymal stem cells (hMSCs), which are often found in the tumor microenvironment where they seem to play a role in tumor progression and drug resistance.

show abstract

Ruthenium metallodendrimers with anticancer potential in an acute promyelocytic leukemia cell line (HL60)

Cited by 39 publications

References 37 publications

Multi-Target Inhibition of Cancer Cell Growth by SiRNA Cocktails and 5-Fluorouracil Using Effective Piperidine-Terminated Phosphorus Dendrimers

Multi-Target Inhibition of Cancer Cell Growth by SiRNA Cocktails and 5-Fluorouracil Using Effective Piperidine-Terminated Phosphorus Dendrimers

Recent advances in metallopolymer-based drug delivery systems

Poly(alkylidenimine) Dendrimers Functionalized with the Organometallic Moiety [Ru(η5-C5H5)(PPh3)2]+ as Promising Drugs Against Cisplatin-Resistant Cancer Cells and Human Mesenchymal Stem Cells

Contact Info

Product

Resources

About