Rutin alleviates cardiomyocyte injury induced by high glucose through inhibiting apoptosis and endoplasmic reticulum stress

Abstract

Diabetic cardiomyopathy is a common complication of diabetes, in which endoplasmic reticulum stress (ERS) serves an important role. Rutin can treat the myocardial dysfunction of diabetic rats. However, to the best of our knowledge, studies on the effects of Rutin on myocardial injury caused by diabetes from the perspective of ERS have not previously been reported. In the present study, the role of rutin in the regulation of ERS in myocardial injury was assessed. Different high glucose concentrations were used to treat H9C2 myoblast cells to establish a myocardial damage model. A cell counting kit-8 assay was used to determine cell viability. A lactate dehydrogenase kit was used to detect cytotoxicity. Apoptosis levels were determined using a TUNEL assay. Western blotting was used to determine the expression levels of apoptosis-related proteins and ERS-related proteins, including heat shock protein A family member 5, inositol-requiring enzyme-1α, X-box binding protein 1, activating transcription factor 6, C/EBP-homologous protein (CHOP), cleaved caspase-12 and caspase-12. The anti-apoptotic and anti-ERS effects of Rutin on H9C2 cardiac cells induced by high glucose were examined after the administration of the ERS activator thapsigargin (TG). The results indicated that rutin could dose-dependently inhibit the level of apoptosis and ERS induced by high glucose in H9C2 cells. After administration of the ERS activator TG, it was demonstrated that TG could reverse the anti-apoptotic and anti-ERS effects of rutin on H9C2 cells stimulated with high glucose. Collectively, the present results suggested that rutin may alleviate cardiomyocyte model cell injury induced by high glucose through the inhibition of apoptosis and ERS.

Mathematical analysis of climatic characteristics of planting areas and biological activity of mulberry branch

“…Similar activities were also found in cryptochlorogenic acid and isoquercitrin (Zhou, 2020). Rutin could alleviate high glucose-induced H9C2 cell damage by inhibiting apoptosis and endoplasmic reticulum stress (Wang, Wang, Li, & Yao, 2021). Hyperoside significantly inhibited apoptosis induced by high glucose in a dose-dependent manner (W. Wu et al, 2020).…”

Protective Effect of Rutin on Triethylene Glycol Dimethacrylate-Induced Toxicity through the Inhibition of Caspase Activation and Reactive Oxygen Species Generation in Macrophages

“…During caecal ligation and puncture surgery, rutin inhibits cardiac apoptosis in high-glucose and sepsis-induced cardiomyopathy [ 33 , 34 ]. Rutin also decreases the rate of apoptosis in the liver and kidneys in rats with deltamethrin-induced hepatotoxicity and nephrotoxicity [ 35 ]. After macrophages are incubated with BisGMA, rutin decreases the rate of apoptosis and necrosis [ 8 ].…”

“…In odontoblast-like cells, TEGDMA decreases the activity of CAT [ 31 ]. Moreover, rutin decreases the level of oxidative stress by ameliorating the activity of SOD and CAT in cadmium-, valproate-, and deltamethrin-induced hepatotoxicity and nephrotoxicity [ 3 , 35 , 55 ]. We observed similar findings with rutin ameliorating the TEGDMA-inhibited activity of SOD and CAT.…”

Flavonoids’ Dual Benefits in Gastrointestinal Cancer and Diabetes: A Potential Treatment on the Horizon?

“…Similarly, rutin also alleviated diabetic complications through apoptosis [ 46 ]. Myoplast cells stimulated with different glucose concentrations and then treated with rutin showed an improvement in cardiomyocyte injury through the direct inhibition of apoptosis and endoplasmic reticulum stress [ 47 ].…”