Rutin and Scopoletin Content and Micropropagation ofFabiana imbricata

Abstract

An HPLC method for the simultaneous quantitative determination of rutin and scopoletin in the aerial parts of FABIANA IMBRICATA is presented. The results showed high variability in the scopoletin (240-2,400 mg%) and rutin (195-1,950 mg%) content in the populations surveyed. A micropropagation method for F. IMBRICATA plants was established by culturing shoot tips on Murashige-Skoog (MS) medium containing 1.0 mg/l benzylaminopurine (BAP), 0.01 mg/l naphthaleneacetic acid (NAA), and 0.1 mg/l gibberellic acid (GA3). Auxin addition to the rooting medium, especially 0.5 mg/l indolebutyric acid (IBA) or 0.5 mg/l IBA and 0.1 mg/l NAA, enhances root formation. The micropropagation method presented allowed the obtention of regenerated plantlets in six weeks from shoot tips.

Minerals, phytic acid, tannin and rutin in buckwheat seed milling fractions

“…Measurement of rutin and quercetin was based on the methods of Terada and Miyabe16 and Razmilic et al 17 Samples, with vanillin as internal standard, were extracted with methanol at 65 °C for 1 h, evaporated to reduce the volume and filtered through 0.2 µm Whatman PVDF filters (Whatman, Clifton, NJ). The samples were analysed by HPLC (high‐pressure liquid chromatography) using a Hewlett Packard series 1100 HPLC equipped with an ultraviolet (UV) detector and an Econosil C18 10U column (250 mm × 4.6 mm) and guard (Alltech, Deerfield, IL) at ambient temperature.…”

Spatial dynamics of <I>Fabiana imbricata</I> shrublands in northwestern Patagonia in relation to natural fires

“…This species forms persistent seed pools in the soil (Gonzalez and Ghermandi, 2008) and its regeneration after a fire depends on seedling production (obligate seeder). The aerial parts of F. imbricata are used for medicinal purposes as a diuretic, digestive agent and to relieve kidney ailments (Razmilic et al, 1994) but it has no value as forage specie for cattle and sheep breeding. This species produce well-demarcated growth rings that can be easily identified (Barichivich et al, 2009).…”

Gastroprotective activity of sesquiterpene derivatives fromFabiana imbricata

“…The Andean shrub Fabiana imbricata R. et P. (Solanaceae) is a medicinal plant known by the common name 'pichi' or 'pichi romero' in Chile and Argentina. An infusion or decoction of the aerial parts is recommended as a diuretic, digestive agent and to relieve kidney ailments (Razmilic et al, 1994). Several biological activities have been reported for the plant, including enzyme inhibition, diuretic and gastroprotective effect (Schmeda-Hirschmann and Papastergiou, 1994;Schmeda-Hirschmann et al, 2004).…”