2007
DOI: 10.1002/elan.200603718
|View full text |Cite
|
Sign up to set email alerts
|

Rutin Determination at an Amperometric Biosensor

Abstract: The feasibility of amperometric detection of rutin at a biosensor using polyphenol oxidase (PPO) as bioelement was investigated. The biosensor was fabricated by entrapment of PPO within a laponite clay film coated on an electrode surface. The amperometric detection of rutin was carried out at À 0.1 V via the direct electrochemical reduction of the product of the enzymatic reaction or its mediated reduction by a phenothiazine redox mediator (Azure B) intercalated within the clay matrix. The biosensor response w… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

0
8
0

Year Published

2009
2009
2019
2019

Publication Types

Select...
6
2

Relationship

0
8

Authors

Journals

citations
Cited by 24 publications
(8 citation statements)
references
References 29 publications
0
8
0
Order By: Relevance
“…273,278,279 In some cases, the clay platelets not only contribute to the long term stability but also may improve the analytical performance. For instance, a biosensor based on laponite/polyphenol oxidase (PPO) biohybrid was able to detect rutin, a citrus flavonoid, which could not be detected with a biosensor prepared simply by chemical cross-linking of PPO onto the electrode surface, 278 this improvement being ascribed to the biocompatibility of the clay material and the high permeability of the laponite-enzyme coating. 280 The immobilization of very large size proteins in the clay interlayer space may be restricted due to the narrow interlayer distance of ca.…”
Section: Functional Proteins and Enzymes Assembled To Silica And Silimentioning
confidence: 99%
“…273,278,279 In some cases, the clay platelets not only contribute to the long term stability but also may improve the analytical performance. For instance, a biosensor based on laponite/polyphenol oxidase (PPO) biohybrid was able to detect rutin, a citrus flavonoid, which could not be detected with a biosensor prepared simply by chemical cross-linking of PPO onto the electrode surface, 278 this improvement being ascribed to the biocompatibility of the clay material and the high permeability of the laponite-enzyme coating. 280 The immobilization of very large size proteins in the clay interlayer space may be restricted due to the narrow interlayer distance of ca.…”
Section: Functional Proteins and Enzymes Assembled To Silica And Silimentioning
confidence: 99%
“…Table shows a comparison of the proposed biosensor with other modified electrodes for rutin determination, based on data recently published in the literature indicating that the proposed methodology is suitable for rutin determination.…”
Section: Resultsmentioning
confidence: 99%
“…Kang et al [6] presented differential pulse voltammetry for rutin by using a glassy carbon electrode (GCE). Mousty et al [7] investigated the amperometric detection of rutin at a polyphenol oxidase based biosensor. Wei et al [8] fabricated a CeO 2 nanoparticle modified electrode for the rutin detection.…”
Section: Introductionmentioning
confidence: 99%