Rutin Exerts Cytotoxic and Senescence-Inducing Properties in Human Melanoma Cells

Pînzaru, Iulia; Chioibaş, Raul; Marcovici, Iasmina; Coricovac, Dorina; Susan, Razvan; Predut, Denisa; Georgescu, D.; Dehelean, Cristina

doi:10.3390/toxics9090226

Cited by 25 publications

(14 citation statements)

References 47 publications

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“…The cytotoxic effects of the BA-GNP were assessed against BA alone in human melanoma RPMI-7951 cells, which can be described as epithelial-like adherent cells able to synthesize and transport melanin and harbor BRAF, PTEN and TP53 mutant genes [34]. The Alamar Blue oxidation-reduction indicator, which quantifies cell viability by changing color in response to chemical reduction due to cell growth, revealed a significant dose-dependent cell death percentage after the application of BA-GNP samples; by contrast, only the highest GNP concentration was able to slightly inhibit cell growth.…”

Section: Discussionmentioning

confidence: 99%

The Anti-Melanoma Effect of Betulinic Acid Functionalized Gold Nanoparticles: A Mechanistic In Vitro Approach

et al. 2022

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Implementing metallic nanoparticles as research instruments for the transport of therapeutically active compounds remains a fundamentally vital work direction that can still potentially generate novelties in the field of drug formulation development. Gold nanoparticles (GNP) are easily tunable carriers for active phytocompounds like pentacyclic triterpenes. These formulations can boost the bioavailability of a lipophilic structure and, in some instances, can also enhance its therapeutic efficacy. In our work, we proposed a biological in vitro assessment of betulinic acid (BA)-functionalized GNP. BA-GNP were obtained by grafting BA onto previously synthesized citrate-capped GNP through the use of cysteamine as a linker. The nanoformulation was tested in HaCaT human keratinocytes and RPMI-7951 human melanoma cells, revealing selective cytotoxic properties and stronger antiproliferative effects compared to free BA. Further examinations revealed a pro-apoptotic effect, as evidenced by morphological changes in melanoma cells and supported by western blot data showing the downregulation of anti-apoptotic Bcl-2 expression coupled with the upregulation of pro-apoptotic Bax. GNP also significantly inhibited mitochondrial respiration, confirming its mitochondrial-targeted activity.

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Section: Discussionmentioning

confidence: 99%

The Anti-Melanoma Effect of Betulinic Acid Functionalized Gold Nanoparticles: A Mechanistic In Vitro Approach

et al. 2022

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“…In brief, the following steps were completed: (i) the cells were cultured in 12-well plates in 1 × 10 5 cells/well number; (ii) the cells were stimulated with three concentrations of NIC (0.1, 10, and 25 μL/mL), after reaching a confluence of 80–90%; (iii) 24 h after this, the cell medium was removed and a volume of 500 μL/well of 1:2000 staining solution diluted in PBS was added; (iv) the plates were placed at room temperature for 10 min in the dark; and (v) the staining solution was washed three times with PBS. For data analysis, 5 μM staurosporin was used as a positive control for the induction of apoptosis, followed by incubation at 37 °C for 3 h. In order to calculate the apoptosis index, the formula described above was employed [ 18 ]:

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Section: Methodsmentioning

confidence: 99%

Nicotine Exerts Cytotoxic Effects in a Panel of Healthy Cell Lines and Strong Irritating Potential on Blood Vessels

Chioran

Sitaru

Macaşoi

et al. 2022

IJERPH

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The use of tobacco products is a major global public health issue, as it is the leading cause of preventable death worldwide. In addition, nicotine (NIC) is a key component of electronic and conventional cigarettes. Although nicotine’s addictive potential is well known, its health effects are not entirely understood. Thus, the main objective of the present study was to evaluate its toxicological profile both in vitro, at the level of three healthy cell lines, and in ovo, at the level of the chorioallantoic membrane. Five different concentrations of nicotine were used in keratinocytes, cardiomyocytes, and hepatocytes for the purpose of evaluating cell viability, cell morphology, and its impact on nuclei. Additionally, the hen’s egg test on the chorioallantoic membrane (HET-CAM) method was used to assess the biocompatibility and irritant potential of the chorioallantoic membrane. Across all cell lines studied, nicotine was proven to be significantly damaging to cell viability, with the highest concentration tested resulting in less than 2% viable cells. Moreover, the morphology of cells changed dramatically, with alterations in their shape and confluence. Nicotine-induced cell death appears to be apoptotic, based on its impact on the nucleus. In addition, nicotine was also found to have a very strong irritating effect on the chorioallantoic membrane. In conclusion, nicotine has an extremely strong toxicological profile, as demonstrated by the drastic reduction of cell viability and the induction of morphological changes and nuclear alterations associated with cellular apoptosis. Additionally, the HET-CAM method led to the observation of a strong irritating effect associated with nicotine.

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“…At micromolar concentrations, rutin possesses a dose-dependent cytotoxic effect against human melanoma cells. This is associated with a reduced viability rate, changes in cellular morphology, apoptotic-like nuclear alterations and reduced confluence [ 149 ]. Rutin can inhibit and regulate the cell cycle in the G2 and G1 phases.…”

Section: Flavonoidsmentioning

confidence: 99%

Secondary Metabolites with Biomedical Applications from Plants of the Sarraceniaceae Family

Miclea

2022

IJMS

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Carnivorous plants have fascinated researchers and hobbyists for centuries because of their mode of nutrition which is unlike that of other plants. They are able to produce bioactive compounds used to attract, capture and digest prey but also as a defense mechanism against microorganisms and free radicals. The main purpose of this review is to provide an overview of the secondary metabolites with significant biological activity found in the Sarraceniaceae family. The review also underlines the necessity of future studies for the biochemical characterization of the less investigated species. Darlingtonia, Heliamphora and Sarracenia plants are rich in compounds with potential pharmaceutical and medical uses. These belong to several classes such as flavonoids, with flavonol glycosides being the most abundant, monoterpenes, triterpenes, sesquiterpenes, fatty acids, alkaloids and others. Some of them are well characterized in terms of chemical properties and biological activity and have widespread commercial applications. The review also discusses biological activity of whole extracts and commercially available products derived from Sarraceniaceae plants. In conclusion, this review underscores that Sarraceniaceae species contain numerous substances with the potential to advance health. Future perspectives should focus on the discovery of new molecules and increasing the production of known compounds using biotechnological methods.

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Rutin Exerts Cytotoxic and Senescence-Inducing Properties in Human Melanoma Cells

Cited by 25 publications

References 47 publications

The Anti-Melanoma Effect of Betulinic Acid Functionalized Gold Nanoparticles: A Mechanistic In Vitro Approach

The Anti-Melanoma Effect of Betulinic Acid Functionalized Gold Nanoparticles: A Mechanistic In Vitro Approach

Nicotine Exerts Cytotoxic Effects in a Panel of Healthy Cell Lines and Strong Irritating Potential on Blood Vessels

Secondary Metabolites with Biomedical Applications from Plants of the Sarraceniaceae Family

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