Rutin potentiates insulin receptor kinase to enhance insulin-dependent glucose transporter 4 translocation

Hsu, Chia‐Yu; Shih, Hung-Yuan; Chia, Yi‐Chen; Lee, Chia‐Hung; Ashida, Hitoshi; Lai, Yiu‐Kay; Weng, Ching‐Feng

doi:10.1002/mnfr.201300691

Cited by 96 publications

(59 citation statements)

References 52 publications

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“…[24] This compound was isolated from the dried power of cultured A. cinnamonea mycelium as was elucidated using a Hitachi Model L-7420 UV/VIS spectrophotometer (Hitachi Ltd., Japan) at 254 nm. [25] (2) Dried C.…”

Section: Bioactive Compounds From Natural Sources Isolationmentioning

confidence: 99%

“…[26] As was separate using a Hibar Lichrosorb RP-18 column (Kanto Chemical Co., Japan, 7 μm; 250x10 mm ID) and chromatographic data were collected and recorded by a D-7000 HPLC System Manager Software (Hitachi Ltd., Japan). [25] There is air-dried, crushed and homogenized. After, analysis of the purity sugarcane wax were carried out using the saponfication (hydrolysis with alkali), calcification, soxhlet refluxing extraction (SRE), purification, and crystallization methods.…”

Section: Bioactive Compounds From Natural Sources Isolationmentioning

confidence: 99%

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Potent Natural Inhibitors of Alpha-Glucosidase and Alpha-Amylase against Hyperglycemia in Vitro and in Vivo

Riyaphan¹,

Jhong²,

Tsai³

et al. 2017

Preprint

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Section: Bioactive Compounds From Natural Sources Isolationmentioning

confidence: 99%

Section: Bioactive Compounds From Natural Sources Isolationmentioning

confidence: 99%

Potent Natural Inhibitors of Alpha-Glucosidase and Alpha-Amylase against Hyperglycemia in Vitro and in Vivo

Riyaphan¹,

Jhong²,

Tsai³

et al. 2017

Preprint

Self Cite

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“…In rat skeletal muscle rutin stimulates Ca 2+ uptake from the extracellular space through voltage-gated Ca 2+ channels and glucose uptake is increased as a consequence of calcium-calmodulin-dependent protein kinase II (CaMKII)-stimulated glucose transporter (GLUT)-4 translocation to the plasma membrane [34]. Rutin has further been shown to enhance insulin-dependent GLUT-4 translocation by insulin-receptor (IR) tyrosin kinase activation [35]. Quercetin and its metabolite quercetin-3-O-methylglucose were shown to protect the umbilical vein endothelium against oxidative stress-induced inflammation and insulin resistance.…”

Section: Introductionmentioning

confidence: 99%

Quercetin Stimulates Insulin Secretion and Reduces the Viability of Rat INS-1 Beta-Cells

Kittl

Beyreis

Tumurkhuu

et al. 2016

Cell Physiol Biochem

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Background/Aims: Previously we described insulinotropic effects of Leonurus sibiricus L. plant extracts used for diabetes mellitus treatment in Traditional Mongolian Medicine. The flavonoid quercetin and its glycoside rutin, which exert anti-diabetic properties in vivo by interfering with insulin signaling in peripheral target tissues, are constituents of these extracts. This study was performed to better understand short- and long-term effects of quercetin and rutin on beta-cells. Methods: Cell viability, apoptosis, phospho-protein abundance and insulin release were determined using resazurin, annexin-V binding assays, Western blot and ELISA, respectively. Membrane potentials (V_mem), whole-cell Ca²⁺ (ICa)- and ATP-sensitive K⁺ (IK_ATP) currents were measured by patch clamp. Intracellular Ca²⁺ (Ca_i) levels were measured by time-lapse imaging using the ratiometric Ca²⁺ indicator Fura-2. Results: Rutin, quercetin and the phosphoinositide-3-kinase (PI3K) inhibitor LY294002 caused a dose-dependent reduction in cell viability with IC₅₀ values of ∼75 µM, ∼25 µM and ∼3.5 µM, respectively. Quercetin (50 µM) significantly increased the percentage of Annexin-V+ cells within 48 hrs. The mean cell volume (MCV) of quercetin-treated cells was significantly lower. Within 2 hrs, quercetin significantly decreased basal- and insulin-stimulated Akt(T308) phosphorylation and increased Erk1/2 phosphorylation, without affecting P-Akt(S473) abundance. Basal- and glucose-stimulated insulin release were significantly stimulated by quercetin. Quercetin significantly depolarized V_mem by ∼25 mV which was prevented by the K_ATP-channel opener diazoxide, but not by the L-type ICa inhibitor nifedipine. Quercetin significantly stimulated ICa and caused a 50% inhibition of IK_ATP. The effects on V_mem, ICa and IK_ATP rapidly reached peak values and then gradually diminished to control values within ∼1 minute. With a similar time-response quercetin induced an elevation in Ca_i which was completely abolished in the absence of Ca²⁺ in the bath solution. Rutin (50 µM) did not significantly alter the percentage of Annexin-V+ cells, MCV, Akt or Erk1/2 phosphorylation, insulin secretion, or the electrophysiological behavior of INS-1 cells. Conclusion: We conclude that quercetin acutely stimulates insulin release, presumably by transient K_ATP channel inhibition and ICa stimulation. Long term application of quercetin inhibits cell proliferation and induces apoptosis, most likely by inhibition of PI3K/Akt signaling.

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“…In rodents diabetic by streptozotocin administration also has exhibit hepatoprotective effect in (Fernandes et al, 2010). In vitro studies show that rutin potentiates insulin receptor kinase (Hsu et al, 2014) and stimulates both the Ca(2+) uptake in rat pancreatic islets (Kappel et al, 2013a) and the glucose uptake in the soleus muscle mediated by extracellular calcium and calciumcalmodulin-dependent protein kinase II (CaMKII) (Kappel et al, 2013b). Being rutin the major flavonoid in P. peruviana calyces, these previous reports suggest its potential hypoglycemic/ antidiabetic activity.…”

Section: Introductionmentioning

confidence: 82%

Development and optimization of microparticles containing a hypoglycemic fraction of calyces from Physalis peruviana

2018

J App Pharm Sci

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Physalis peruviana L. (Cape gooseberry) is a plant widely cultivated in the Andean mountain in South America and its calyces are the main by-product of the harvest and commercialization of the fruits. Hypoglycemic activity was evaluated to the hydroethanolic extract and its fractions by glucose tolerance test. Since butanolic fraction exhibited hypoglycemic activity as well as the hydroethanolic extract, this fraction was microencapsulated in hypromellose phthalate (HPMCP) by the emulsification-evaporation method. Rutin was the major flavonoid in the whole extract and butanolic fraction. By using Plackett-Burman and Box-Behnken statistical experimental designs, were developed and optimized HPMCP-microparticles loaded with butanolic fraction. Optimized microparticles have an entrapment efficiency of 71% and yield of 64%, only 8% of release at acidic pH and significant hypoglycemic activity. Development and optimization of microencapsulated butanolic fraction of calyces from P. peruviana is an important contribution to improve its hypoglycemic activity as a promising therapeutic strategy.

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Rutin potentiates insulin receptor kinase to enhance insulin-dependent glucose transporter 4 translocation

Abstract: This study shows evidence that rutin may serve as a potential agent for glycemic control through enhancement of IRK activity, thereby inducing the insulin signaling pathway causing increased GLUT4 translocation and increased glucose uptake.

Cited by 96 publications

References 52 publications

Potent Natural Inhibitors of Alpha-Glucosidase and Alpha-Amylase against Hyperglycemia <em>in Vitro </em>and <em>in Vivo</em>

Potent Natural Inhibitors of Alpha-Glucosidase and Alpha-Amylase against Hyperglycemia <em>in Vitro </em>and <em>in Vivo</em>

Quercetin Stimulates Insulin Secretion and Reduces the Viability of Rat INS-1 Beta-Cells

Development and optimization of microparticles containing a hypoglycemic fraction of calyces from Physalis peruviana

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