RuvB-Like Protein 2 (Ruvbl2) Has a Role in Directing the Neuroectodermal Differentiation of Mouse Embryonic Stem Cells

Abstract

Although many factors have been identified to be involved in the development of the neuroectoderm during embryogenesis, it is still important to identify novel factors that convert undifferentiated embryonic cells into neuroectoderm. RuvB-like protein 2 (Ruvbl2) is known to regulate gene expression via chromatin remodeling by participating in multi-protein complexes, but its role during embryonic development is not well known. In this study, we established Ruvbl2-overexpressing mouse embryonic stem cells and examined their capacity to specifically differentiate into neuroectoderm and confirmed the specific expression of RUVBL2 in early embryonic neuroectoderm. Our results suggest that Ruvbl2 has a role in the differentiation of neuroectoderm during early embryogenesis.

MBTD1 preserves adult hematopoietic stem cell pool size and function

“…6 B and SI Appendix , Table S3 ). Of note, among repeatedly identified proteins, we found molecules that have been reported to be involved in HSC or other stem cell functions, which included proteins belonging to TIP60 complex (EP400, TRPPAP, RUVBL1, and RUVBL2) ( 30 – 33 ), ribosomal proteins (RPL17, RPL18, and RPS19) ( 34 ), heat shock protein (HSPA9) ( 35 ), Calmodulin (CaM) ( 36 , 37 ), and an mRNA regulator (DDX6) ( 38 ) (blue circle in Fig. 6 B and SI Appendix , Table S3 ).…”

“…6 D ). Among them, TIP60 complex constituents (EP400, EPC1, TRPPAP, MRGBP, RUVBL1, and RUVBL2), ribosomal proteins (RPL17, RPL18, and RPS19), heat shock protein (HSPA9), and Calmodulin (CaM) have been reported to be essential for HSC function ( 30 – 37 ). Interestingly, MBTD1 deficiency-induced abnormal protein synthesis was partly recovered by FoxO3a TM ( Fig.…”

Characterization of the microRNA Transcriptomes and Proteomics of Cochlea Tissue-derived Small Extracellular Vesicles From Different Age of Mice After Birth

“…Among the proteins that decrease with age, Hnrnp [110], Ddx5 [111], Ilf3 [112], Lamtor5 [113], Psmd2 [114], Ddb1 [115], Psmd6 [116], and Chd4 [117] are reported to related to cell proliferation and differentiation. Ptbp1 [118], Chd4 [119], Ruvbl2 [120], Cul4a [121], and Lama4 [122] are required for early developmental processes and neuronal differentiation, and some proteins also presented in the inner ear, such as P3h1 [123], Sorcs2 [78], Panx3 [124,125], Idh1 [126], Lamb1 [127]. P3h1 knockout mice showed dysplasia of middle ear bones and hearing impairment [123].…”