S-100 protein-immunoreactive cells in the lymph node and spleen of the rat.

Iwanaga, Toshihiko; Fujita, Tsuneo; Masuda, Tohru; Takahashi, Yasuo

doi:10.1679/aohc.45.393

Cited by 21 publications

(5 citation statements)

References 18 publications

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“…Those findings are mostly obtained by use of anti-S-100 serum raised in the rabbit against bovine brain S-100 protein as well as this experiment. In the duck, unlike the mammal (Iwanaga et al, 1982;Haimoto et al, 1987), no S-100 protein was detected in lymphoid tissues or in the thyroid, parathyroid, adrenal glands or cartilage. Furthermore, there was disagreement of cell types containing S-100 protein in avian and mammalian tissues; for example, in the islets of Langerhans of the duck it was suggested that B cells and D cells contain S-100 protein, because all cells of the beta islet were immunoreactive for this protein and in the alpha islets, S-100-protein-positive cells coincide with argyrophilic cells.…”

Section: Discussionmentioning

confidence: 96%

S‐100 immunoreactive cells in non‐nervous duck tissues

et al. 1989

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S-100 protein was detected in endocrine cells of the islet of Langerhans, hypophysis, ultimobranchial body, proventriculus, gizzard and intestine, and epithelial cells of the interlobular bile duct, gall bladder, renal tubules and epididymal and urinary ducts except for the nerve elements in the duck. Sertoli cells and oocytes were also S-100-protein immuno-reactive. The types of S-100-protein-positive cells detected in the duck differ from those of mammalian species.

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Section: Discussionmentioning

confidence: 96%

S‐100 immunoreactive cells in non‐nervous duck tissues

et al. 1989

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“…In contrast, only a small, approximately 10-30% fraction of these neurons bind tetanus toxin [unpublished observations]. S-100 was employed as a marker for satellite cells [weiss et al, 1983;Ushiki et al, 19841 assuming that other non-neuronal cells that may express S-100 [Iwanaga et al, 1982;Stefansson et al, 1982;Michetti et al, 19831 were absent from DRG cell dissociates. Round phase-bright cells indicative of being neurons were not stained by antis-100 antibodies.…”

Section: Characterization Of Neuron-enriched Culturesmentioning

confidence: 99%

Neuron‐Enriched cultures of adult rat dorsal root ganglia: Establishment, characterization, survival, and neuropeptide expression in response to trophic factors

Grothe

Unsicker

1987

J of Neuroscience Research

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It is unknown whether adult dorsal root ganglion (DRG) neurons require trophic factors for their survival and maintenance of neuropeptide phenotypes. We have established and characterized neuron-enriched cultures of adult rat DRGs and investigated their responses to nerve growth factor (NGF), ciliary neuronotrophic factor (CNTF), pig brain extract (PBE, crude fraction of brain-derived neuronotrophic factor, BDNF), and laminin (LN). DRGs were dissected from levels C1 through L6 and dissociated and freed from myelin fragments and most satellite (S-100-immunoreactive) cells by centrifugation on Percoll and preplating. The enriched neurons, characterized by their morphology and immunoreactivity for neuron-specific enolase, constituted a population representative of the in vivo situation with regard to expression of substance P (SP), somatostatin (SOM), and cholecystokinin-8 (CCK) immunoreactivities. In the absence of trophic factors and using polyornithine (PORN) as a substratum, 60-70% of the neurons present initially (0.5 days) had died after 7 days. LN as a substratum did not prevent a 30% loss of neurons up to day 4.5, but it subsequently maintained DRG neurons at a plateau. This behavior might reflect a cotrophic effect of LN and factors provided by non-neuronal cells, whose proliferation between 4.5 and 7 days could not be prevented by addition of mitotic inhibitors of gamma-irradiation. CNTF, but not NGF, slightly enhanced survival at 7 days on either PORN or LN. No neuronal losses were found in non-enriched cultures or when enriched neurons were supplemented with PBE, indicating that non-neuronal cells and PBE provide factor(s) essential for adult DRG neuron survival. Proportions of SP-, SOM-, and CCK-immunoreactive cells were unaltered under any experimental condition, with the exception of a numerical decline in SP cells in 7-day cultures with LN, but not PORN, as the substratum. Our data, considered in the context of recent in vivo and vitro studies, suggest that a combination of trophic factors or an unidentified factor, rather than the established molecules NGF, CNTF, and BDNF, which address embryonic and neonatal DRG neurons, are required for the in vitro maintenance of adult DRG neurons.

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“…This evidence indicates typical presence of S-100 protein in the immune system, since the species difference of the antigenicity is apparently ruled out. Several studies have revealed S-100 immunoreactive cell types in the lymph node and spleen of some species: the follicular dendritic cells, interdigitating cells or macrophages (Iwanaga et al, 1982;Vanstapel et al, 1986;Haimoto et al, 1987;Sugimura et al, 1987). In these studies, cell population of S-100 immunoreactivity tends to predominate in the germinal center and paracortical area.…”

Section: Discussionmentioning

confidence: 98%

“…However, immunohistochemical studies have recently demonstrated S-100 protein in many cell types of non-nervous tissues of various mammalian species. In lymphoid tissues, S-100 protein was restricted in non-lymphoid cells; the follicular dendritic cells (Iwanaga et al, 1982;Cocchia et al ., 1983;Haimoto et al, 1987;Suigmura et al, 1987), interdigitating cells (Pelletier et al, 1986;Haimoto et al, 1987;Sugimura et al, 1987) and tingible body macrophages (Takahashi et al, 1984;Sugimura et al, 1987). However, species difference of S-100 immunoreactivity is confirmed in non-lymphoid cell types of animals examined.…”

mentioning

confidence: 99%

Immunohistochemical Study of S-100 Protein in the Bovine Lymph Node and Spleen

Atoji

Suzuki

1990

Okajimas Folia Anatomica Japonica

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Immunohistochemistry using anti-bovine S-100 protein serum was examined in the bovine lymph node and spleen. In the lymph node, immunoreactivity was found in endothelial cells of lymph vessels and in endothelial and reticular cells of the sinuses. In the spleen, immunoreactivity was observed in endothelial cells of the trabecular artery, central artery, penicillar artery, sheathed artery, terminal capillary, trabecular vein and lymph vessel. In addition, the follicular dendritic cells in germinal centers both of the lymph node and spleen were stained with S-100 protein. These findings suggest that S-100 protein of the vascular systems may be related to the flow of lymph and blood.

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S-100 protein-immunoreactive cells in the lymph node and spleen of the rat.

Cited by 21 publications

References 18 publications

S‐100 immunoreactive cells in non‐nervous duck tissues

S‐100 immunoreactive cells in non‐nervous duck tissues

Neuron‐Enriched cultures of adult rat dorsal root ganglia: Establishment, characterization, survival, and neuropeptide expression in response to trophic factors

Immunohistochemical Study of S-100 Protein in the Bovine Lymph Node and Spleen

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