S‐180 cells secrete nerve growth factor protein similar to 7S‐nerve growth factor

Abstract

The nerve growth factor protein (NGF) has been identified by biological assay and rocket immunoelectrophoresis in media conditioned by monolayers of mouse S-180 sarcoma cells, a transformed cell line of salivary gland origin. By utilization of a purification procedure designed to enrich for acid-dissociable, high-molecular-weight complexes similar to the 7S-NGF isolated from male mouse submaxillary gland, it has been determined that a significant portion of mouse sarcoma NGF is initially present as a complex at neutral pH with a molecular weight indistinguishable from that of 7S-NGF. At pH 4.0 the mouse sarcoma NGF complex dissociates, and the active subunit can be isolated as a species with a molecular weight of less than 40,000. The induced dissociation at pH 4.0 of the mouse sarcoma NGF high-molecular-weight complex, as well as the complex's behavior in isoelectric focusing and sucrose gradient sedimentation is consistent with the hypothesis that 7S-NGF is packaged as a subunit containing protein intracellularly prior to secretion into the extracellular space. Moreover, the stability of the mouse sarcoma NGF complex at dilute concentrations is similar to that reported for the purified 7S-NGF complex.

Developmental Neurobiology and the Natural History of Nerve Growth Factor

“…He identified the NGF of snake venom and of the mouse salivary glands in two protein moieties, both of which were found to possess biological and biochemical properties remarkably similar to the NGF of mouse sarcomas. Recent studies have provided new evidence to support this point (Barklis & Perez-Polo 1981). The discovery that NGF is synthesized in the collecting tubules of the salivary glands (Levi-Montalcini & Angeletti 1961) and that the synthesis is controlled by testosterone (Levi-Montalcini & Angeletti 1964, Ishii & Shooter 1975 and by thyroxine (Aloe & Levi-Montalcini 1980) has contributed further to the studies of NGF.…”

Cell Culture in the Neurosciences

“…The advantage of this approach is that characterization of the NTF molecular species can be obtained in vivo prior to complete purification. Young et al (1975) Murphy et al (1975), Schwartz and Costa (1978) Barklis andPerez-Polo (1981) Oger et al (1974), Pantazis et al (1977) Murphy et a/. (1974), Perez-Polo et al (1977), , Some tissues contain neuronotrophic activity that can act on the neurons innervating that tissue.…”