S-adenosyl-l-methionine:trans-caffeoyl-coenzyme A 3-O-methyltransferase from elicitor-treated parsley cell suspension cultures

Pakusch, Anne-Elisabeth; Kneusel, Richard E.; Matern, Ulrich

doi:10.1016/0003-9861(89)90299-3

Cited by 116 publications

(86 citation statements)

References 21 publications

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“…bation with INA. The ferulic cell wall reinforcement is commonly associated with browning of the cells a n d depends on the hydroxylation of 4-coumaroyl-to caffeoyl-COA as shown originally in parsley (Kneusel et al, 1989;Matern et al, 1995); this pathw a y has meanwhile been confirmed for m a n y other plants. The hydroxylation is controlled b y the cytoplasmic pH, which decreases upon fungal infection o r elicitation (Kneuse1 e t al., 1989).…”

Section: Putative Role Of Ccoaomt In the Sar Response Of V Viniferamentioning

confidence: 76%

“…CCoAOMT activity was assayed according to the method of Pakusch et al (1989) using caffeoyl-CoA or 5-hydroxyferuloyl-COA as a substrate, and COMT activity was measured under equivalent conditions with caffeate or 5-hydroxyferulate as substrate. Crude enzyme extracts were prepared by homogenization of deep-frozen V. vinifera cells in extraction buffer and clearing by centrifugation (1O,OOOg, 10 min).…”

Section: Ccoaomt and Comt Assaysmentioning

confidence: 99%

“…These results already point to a close link between SAR and the sensitization of phenylpropanoid metabolism, although the fact that the tissue phenotype in the SAR state was unchanged and required an additional fungal inoculation to release the enhanced lignification remains puzzling. The reinforcement with lignin-like materials requires the synthesis of feruloyl-and sinapoyl-CoAs (Walter, 1992;Boudet et al, 1995) and a nove1 route to these substrates was reported recently based on the sequential methoxylations of 4-coumarate:CoA ligase (Kneusel et al, 1989;Pakusch et al, 1989Matern et al, 1995). The methyltransferase involved, CCoAOMT, was cloned, and the expression of the enzyme activity appeared to be controlled by multiple parameters (Schmitt et al, 1991;Ye et al, 1994;Grimmig and Matern, 1997).…”

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confidence: 99%

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Characterization and Expression of Caffeoyl-Coenzyme A 3-O-Methyltransferase Proposed for the Induced Resistance Response of Vitis vinifera L

et al. 1997

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Cell-suspension cultures of Vitis vinifera L. cv Pinot Noir accumulated resveratrol upon fungal elicitation, and the activity of S-adenosyl-i-methi0nine:trans-caffeoyl-coenzyme A 3-O-methyltransferase (CCoAOMT), yielding feruloyl-COA, increased to a transient maximum at 12 to 15 h. CCoAOMT cDNA was cloned from the elicited cells and was shown to encode a polypeptide highly homologous to CCoAOMTs from cells of Petroselinum species or Zinnia species. The expression of the cDNA i n Escherichia coli revealed that grapevine CCoAOMT methylates both caffeoyland 5-hydroxyferuloyl-coenzyme A and is probably involved in phenolic esterification and lignification. Commercial plant activators induce the disease-resistance response of test plants and are considered to mimic the action of salicylic acid. Among these chemicals, 2,6-dichloroisonicotinic acid and benzo(l,2,3)-thiadiazole-7-carbothioic acid S-methyl ester provoke systemic acquired resistance (SAR) and were also shown to induce the expression of class III chitinase i n grapevine. The SAR response is classified by an unchanged phenotype of tissues, but the mechanistic basis is unknown. Treatment of the cultured V. vinifera cells with either fungal elicitor or low concentrations of salicylic acid and 2,6-dichloroisonicotinic acid, respectively, raised the CCoAOMT or stilbene synthase transcript abundance, suggesting that grapevine is capable of the SAR response, whereas benzo(l,2,3)-thiadiazole-7-carbothioic acid S-methyl ester was ineffective. The data imply for the first time (to our knowledge) that the expression of phenylpropanoid genes in grapevine i s induced by SAR activators without phenotypic consequences and suggest a role for CCoAOMT and stilbene synthase in the disease-resistance response leading beyond the leve1 of pathogenesis-related proteins as markers of the SAR.Plants respond to local fungal infection by the activation of various defense measures in the challenged tissues, and the induction of phenylpropanoid pathways appears to play a crucial role in this response (Hahlbrock and Scheel, 1989). The activation causes the short-term accumulation of phenolic metabolites, which might possess potent antimycotic activity as such or may protect the cells indirectly after incorporation into the cell wall. The phenolic materi-

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Section: Putative Role Of Ccoaomt In the Sar Response Of V Viniferamentioning

confidence: 76%

Section: Ccoaomt and Comt Assaysmentioning

confidence: 99%

mentioning

confidence: 99%

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Characterization and Expression of Caffeoyl-Coenzyme A 3-O-Methyltransferase Proposed for the Induced Resistance Response of Vitis vinifera L

et al. 1997

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“…HCT-down-regulated alfalfa also enhances disease resistance to the fungus Colletotrichum trifolii, the causal agent of alfalfa anthracnose (GallegoGiraldo et al, 2011b). In parsley (Petroselinum crispum) suspension cells, the enzymatic activity of CCoAOMT rapidly increased after fungal elicitor treatment (Pakusch et al, 1989). Down-regulation of CCoAOMT in N. benthamiana results in higher susceptibility to Pseudomonas syringae pv tabaci (Senthil-Kumar and Mysore, 2010), and silencing of CCoAOMT in wheat (Triticum aestivum) compromises the defense against powdery mildew (Bhuiyan et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Maize Homologs of CCoAOMT and HCT, Two Key Enzymes in Lignin Biosynthesis, Form Complexes with the NLR Rp1 Protein to Modulate the Defense Response

Wang

Balint‐Kurti²

2016

Plant Physiol.

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Disease resistance (R) genes encode nucleotide binding Leu-rich-repeat (NLR) proteins that confer resistance to specific pathogens. Upon pathogen recognition they trigger a defense response that usually includes a so-called hypersensitive response (HR), a rapid localized cell death at the site of pathogen infection. Intragenic recombination between two maize (Zea mays) NLRs, Rp1-D and Rp1-dp2, resulted in the formation of a hybrid NLR, Rp1-D21, which confers an autoactive HR in the absence of pathogen infection. From a previous quantitative trait loci and genome-wide association study, we identified genes encoding two key enzymes in lignin biosynthesis, hydroxycinnamoyltransferase (HCT) and caffeoyl CoA O-methyltransferase (CCoAOMT), adjacent to the nucleotide polymorphisms that were highly associated with variation in the severity of Rp1-D21-induced HR. We have previously shown that the two maize HCT homologs suppress the HR conferred by Rp1-D21 in a heterologous system, very likely through physical interaction. Here, we show, similarly, that CCoAOMT2 suppresses the HR induced by either the full-length or by the N-terminal coiled-coil domain of Rp1-D21 also likely via physical interaction and that the metabolic activity of CCoAOMT2 is unlikely to be necessary for its role in suppressing HR. We also demonstrate that CCoAOMT2, HCTs, and Rp1 proteins can form in the same complexes. A model is derived to explain the roles of CCoAOMT and HCT in Rp1-mediated defense resistance.

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“…In04 encoded caffeoyl-CoA-3-Omethyltransferase, an enzyme that catalyses the methylation of caffeoyl-CoA into feruoyl-CoA, a precursor of lignin. It is involved in growth and in disease resistance response (Pakusch et al 1989). Flower opening is based on movements and therefore on elongation growth.…”

Section: Appearance Of Senescence Parameters Compared With Gene Exprementioning

confidence: 99%

Gene expression in opening and senescing petals of morning glory (Ipomoea nil) flowers

et al. 2007

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We isolated several senescence-associated genes (SAGs) from the petals of morning glory (Ipomoea nil) flowers, with the aim of furthering our understanding of programmed cell death. Samples were taken from the closed bud stage to advanced visible senescence. Actinomycin D, an inhibitor of transcription, if given prior to 4 h after opening, suppressed the onset of visible senescence, which occurred at about 9 h after flower opening. The isolated genes all showed upregulation. Two cell-wall related genes were upregulated early, one encoding an extensin and one a caffeoyl-CoA-3-O-methyltransferase, involved in lignin production. A pectinacetylesterase was upregulated after flower opening and might be involved in cell-wall degradation. Some identified genes showed high homology with published SAGs possibly involved in remobilisation processes: an alcohol dehydrogenase and three cysteine proteases. One transcript encoded a leucine-rich repeat receptor protein kinase, putatively involved in signal transduction. Another transcript encoded a 14-3-3 protein, also a protein kinase. Two genes have apparently not been associated previously with senescence: the first encoded a putative SEC14, which is required for Golgi vesicle transport, the second was a putative ataxin-2, which has been related to RNA metabolism. Induction of the latter has been shown to result in cell death in yeast, due to defects in actin filament formation. The possible roles of these genes in programmed cell death are discussed.

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S-adenosyl-l-methionine:trans-caffeoyl-coenzyme A 3-O-methyltransferase from elicitor-treated parsley cell suspension cultures

Cited by 116 publications

References 21 publications

Characterization and Expression of Caffeoyl-Coenzyme A 3-O-Methyltransferase Proposed for the Induced Resistance Response of Vitis vinifera L

Characterization and Expression of Caffeoyl-Coenzyme A 3-O-Methyltransferase Proposed for the Induced Resistance Response of Vitis vinifera L

Maize Homologs of CCoAOMT and HCT, Two Key Enzymes in Lignin Biosynthesis, Form Complexes with the NLR Rp1 Protein to Modulate the Defense Response

Gene expression in opening and senescing petals of morning glory (Ipomoea nil) flowers

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