S-Allylcysteine, a garlic-derived antioxidant, ameliorates quinolinic acid-induced neurotoxicity and oxidative damage in rats

Pérez-Severiano, Francisca; Rodrı́guez-Pérez, Mayra; Pedraza‐Chaverrí, José; Maldonado, Perla D.; Medina-Campos, Omar Noel; Ortíz-Plata, Alma; Sánchez-García, Aurora; Villeda‐Hernández, Juana; Galván‐Arzate, Sonia; Aguilera, Penélope; Santamarı́a, Abel

doi:10.1016/j.neuint.2004.06.008

Cited by 147 publications

(79 citation statements)

References 41 publications

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“…Studies on cellular insults responsible for ER stress-induced cell death in human origin cells is also in progress in our laboratory. SAC, an organosulfur compound possessing a thioallyl group, present in AGE, has been shown to protect neurons against various toxic or ischemic conditions (7,21,35). Previous investigations in our laboratory have also shown that SAC protects NGF-differentiated PC12 cells (22) and cultured hippocampal neurons (3) against Aβ-and tunicamycin-induced toxicities.…”

Section: Discussionmentioning

confidence: 82%

“…S-Allyl-Lcysteine (SAC), an active organosulfur compound present in AGE, has been shown to have neurotrophic effects (20) in cultured neurons and neuroprotective activities (21) in the rodent brain. In our studies, we have found that SAC protects against Aβ-induced cell death, but does not protect against 4-hydroxynonenalinduced cell death in either NGF-differentiated PC12 cells (22) or cultured hippocampal neurons (3).…”

Section: Introductionmentioning

confidence: 99%

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Role of Caspase-12 in Amyloid β-Peptide-Induced Toxicity in Organotypic Hippocampal Slices Cultured for Long Periods

et al. 2007

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Abstract. Amyloid β (Aβ) toxicity has been implicated in cell death in the hippocampus, but its specific mechanisms are poorly understood. In this study, Aβ-induced cell death was investigated in organotypic hippocampal slice cultures (OHCs) that were cultured for various periods in vitro. There were no obvious histological differences among slices cultured for 3 to 7 weeks in vitro. Although there was little neurotoxicity after treatment with Aβ 25-35 in OHCs cultured for relatively shorter periods (3 -5 weeks), age-dependent cell death was evident in OHCs cultured for relatively longer periods (6 -7 weeks) after exposure to Aβ 25-35 . In OHCs cultured for 7 weeks, S-allyl-L-cysteine (SAC), a component of aged garlic extract, protected the cells in areas CA1 and CA3 and the dentate gyrus from Aβ 25-35 -induced toxicity. The immunoreactivity of cleaved caspase-12 was increased whereas that of glucose-regulated protein 78 was not altered after exposure to Aβ [25][26][27][28][29][30][31][32][33][34][35] . The increases in the cleaved caspase-12 were also reversed by simultaneously applied SAC. These results suggest that OHCs cultured for relatively longer periods are more susceptible to Aβ-induced toxicity and that the Aβ-induced cell death involves caspase-12-dependent pathways. It is also suggested that SAC is able to protect against the Aβ-induced neuronal cell death through the inhibition of the caspase-12-dependent pathway.

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Section: Discussionmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Role of Caspase-12 in Amyloid β-Peptide-Induced Toxicity in Organotypic Hippocampal Slices Cultured for Long Periods

et al. 2007

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“…2 0 -7 0 -Dichlorofluorescein diacetate (DCF-DA) oxidation was used as a general index of OS as described by Perez-Severiano et al (2004). Groups of 20 flies were mechanically homogenized in 1 mL 20 mM Tris buffer (pH 7.0), and centrifuged at 1600g for 10 min, 4 C. The supernatant was used to quantify DCF-DA oxidation.…”

Section: Dcf-da Oxidation Assaymentioning

confidence: 99%

“…The measurement of DCF-DA oxidation is an indicator of general OS (Perez-Severiano et al 2004). After 72 h of treatment with HECC, there was an augmented level of ROS formation from 0.1 mg/mL.…”

Section: Dcf-da Oxidation Assay and Activity Of Enzymes Cat Sod Gstmentioning

confidence: 99%

Oxidant effects and toxicity ofCroton campestrisinDrosophila melanogaster

Júnior

Macedo

Zemolin

et al. 2016

Pharmaceutical Biology

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Context: Croton campestris A.St.-Hil. (Euphorbiaceae) is a species native to Northeast Brazil used by traditional communities for the treatment of a variety of health problems. However, potential toxicological effects of this plant are unknown. Objective: The potential toxicity of the hydroalcoholic extract of C. campestris leaves on Drosophila melanogaster insect model, additionally with phytochemical constitution and cellular mechanisms mediating the action of extract were analysed in this study. Materials and methods: Constituents of the extract were evaluated by HPLC. In vitro antioxidant potential of extract was analysed by DPPH, ABTS and FRAP. Flies injected culture medium mixed with extract (0.1-50 mg/mL) for 72 h. After, ROS production was evaluated by DCF-DA oxidation. Phosphorylation of MAPK signalling pathway was investigated by Western blotting method. Activity of antioxidant enzymes was analysed in homogenates.Results: Major components of the extract include quercetin (38.11 ± 0.06 mg/g), caffeic acid (20.06 ± 0.17 mg/g) and kaempferol (15.45 ± 0.05 mg/g). Consumption of the extract impaired locomotor performance and induced fly death of flies (LC 50 of 26.51 mg/mL). Augmented ROS formation and SOD, CAT and GST activity were observed from 0.1 mg/mL. JNK and p38 kinases phosphorylation was modulated and Paraquat-induced toxicity was augmented by extract. Discussion and conclusion: Our data show important toxicological effects of C. campestris leading to increased mortality and impaired locomotor performance accompanied by induction of cell stress markers in flies. The study draws attention to the indiscriminate use of plant extracts. ARTICLE HISTORY

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“…Many of the physiological effects of garlic are attributed to www.intechopen.com the volatile sulfur compounds like thiosulfinates, which are also responsible for its pungent aroma. Recently, the cardioprotective ability of garlic was attributed to s-allylcysteine (Perez-Severiano et al, 2004). Blood pressure reducing properties of garlic have been linked to its hydrogen sulphide production and allicin content -liberated from alliin and the enzyme alliinase which has angiotensin II inhibiting and vasodilating effects, as shown in animal and human cell studies.…”

Section: Allium Sativum (Garlic)mentioning

confidence: 99%

Evaluation of Anti-Ischemic Therapy in Coronary Artery Disease: A Review

Al-Nimer¹

2012

Coronary Artery Diseases

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S-Allylcysteine, a garlic-derived antioxidant, ameliorates quinolinic acid-induced neurotoxicity and oxidative damage in rats

Cited by 147 publications

References 41 publications

Role of Caspase-12 in Amyloid β-Peptide-Induced Toxicity in Organotypic Hippocampal Slices Cultured for Long Periods

Role of Caspase-12 in Amyloid β-Peptide-Induced Toxicity in Organotypic Hippocampal Slices Cultured for Long Periods

Oxidant effects and toxicity ofCroton campestrisinDrosophila melanogaster

Evaluation of Anti-Ischemic Therapy in Coronary Artery Disease: A Review

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