S-Antigen Antibody Partially Blocks Entrainment and the Effects of Constant Light on the Circadian Rhythm of Locomotor Activity in the Adult Blow Fly, Calliphora vicina

Cymborowski, B.; Hong, Seau-Feng; McWatters, Harriet G.; Saunders, D. S.

doi:10.1177/074873049601100107

Cited by 15 publications

(5 citation statements)

References 16 publications

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“…As a result, 96% of total activity was concentrated in the photophase, indicating that this species is principally diurnal. Similar restriction of activity to the photophase was also observed for other flies (Helfrich et al 1985;Cymborowski et al 1996). Such diurnally active patterns are in stark contrast to the crepuscular type of locomotor behavior displayed by wild-type D. melanogaster, which account for 53% of total activity in the dark phase (e.g., Figure 1C).…”

Section: Resultssupporting

confidence: 76%

Comparative Analysis of Pdf-Mediated Circadian Behaviors BetweenDrosophila melanogasterandD. virilis

2009

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A group of small ventrolateral neurons (s-LN v 's) are the principal pacemaker for circadian locomotor rhythmicity of Drosophila melanogaster, and the pigment-dispersing factor (Pdf) neuropeptide plays an essential role as a clock messenger within these neurons. In our comparative studies on Pdf-associated circadian rhythms, we found that daily locomotor activity patterns of D. virilis were significantly different from those of D. melanogaster. Activities of D. virilis adults were mainly restricted to the photophase under light:dark cycles and subsequently became arrhythmic or weakly rhythmic in constant conditions. Such activity patterns resemble those of Together these findings suggest a significant difference in the regulatory mechanisms of Pdf transcription between the two species and such a difference is causally associated with species-specific establishment of daily locomotor activity patterns.

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Section: Resultssupporting

confidence: 76%

Comparative Analysis of Pdf-Mediated Circadian Behaviors BetweenDrosophila melanogasterandD. virilis

2009

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“…This sensitivity is best characterized by a phase response curve that plots magnitude and direction (advance or delay) of the phase shifts caused by single pulse, as a function of the phase in the circadian cycle at which the pulses are given. ( Pittendrigh, 1960 ; Page, 1987 ; Cymborowski et al, 1993 ; Cymborowski et al, 1996 ). In contrast to the light pulse phase response curve, that for 5,7-DHT treatment is of “inverted” type with phase delay in the early subjective day and phase advance in the late subjective day (see Figures 1 and 2 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of 5,7-dihydroxytriptamine (5,7-DHT) on circadian locomotor activity of the blow fly, Calliphora vicina

Cymborowski¹

2003

Journal of Insect Science

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The biogenic amine serotonin (5-HT) is a neuromodulator in both vertebrates and invertebrates. It has been shown that serotonin, apart from its distinct effects on behavior, also plays a morphoregulatory role during the ontogeny of the insect's nervous system. The role of serotonin in modulating circadian locomotor activity of the blow fly, Calliphora vicina was explored. Injection of a specific neurotoxin, 5,7-dihydroxytryptamine (5,7-DHT), into the hemolymph appeared to significantly reduced the level of locomotor activity and lengthened the period (τ) of circadian rhythmicity. After drug injection in constant darkness flies continued with their free-running rhythm of a locomotor activity, depending on the time of 5,7-DHT injection. This compound causes phase delay when administered in the early subjective day, and phase advance in the late subjective day. This effect is the opposite of the phase response curve obtained for 5-HT injections. This suggests that 5-HT might act as an entraining agent via the output pathway by feedback to clock neurons in the brain. Some of the injected insects regained their normal level of activity after a few days. These findings suggest a potential role for serotonin as modulator of circadian rhythms in insect including regulation of the level of locomotor activity.5-HT5-hydroxytryptamine, serotonin5,7-DHT5,7-dihydroxytryptamine

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“…In the last decade of the twentieth century, David’s research interests focused on determining the anatomical location of circadian and photoperiodic photoreceptors and circadian clocks, which led to a series of experiments with Calliphora vicina , involving a collaboration with colleagues from Warsaw, North Carolina, Auckland and Taiwan (Cymborowski et al 1994 , 1996 ; Saunders and Cymborowski 1996 , 2003 ; Hong and Saunders 1998 ).…”

Section: Scientific Workmentioning

confidence: 99%

David S. Saunders: man of insects and photoperiodism (1935–2023)

Helfrich‐Förster

2023

J Comp Physiol A

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David S. Saunders was an outstanding scientist, who devoted his life to his family and to insects. He has made many fundamental contributions to our understanding of how insects reproduce and adapt their reproduction and development to the seasonal changes on our planet. Most importantly, he was a pioneer in demonstrating the role of the circadian clock in insect photoperiodic time measurement, first in the jewel wasp Nasonia vitripennis, and later in varies species of flies. His books on biological rhythms and insect clocks are important undergraduate, graduate and research reference literature. David was also a brilliant teacher and mentor and played a major role in establishing and teaching a series of successful Erasmus-funded Chronobiology Summer Schools in Europe. He leaves behind a legacy, both professionally and personally.

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S-Antigen Antibody Partially Blocks Entrainment and the Effects of Constant Light on the Circadian Rhythm of Locomotor Activity in the Adult Blow Fly, Calliphora vicina

Cited by 15 publications

References 16 publications

Comparative Analysis of Pdf-Mediated Circadian Behaviors BetweenDrosophila melanogasterandD. virilis

Comparative Analysis of Pdf-Mediated Circadian Behaviors BetweenDrosophila melanogasterandD. virilis

Effects of 5,7-dihydroxytriptamine (5,7-DHT) on circadian locomotor activity of the blow fly, Calliphora vicina

David S. Saunders: man of insects and photoperiodism (1935–2023)

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