S-phase kinase-associated protein 2 knockdown blocks colorectal cancer growth via regulation of both p27 and p16 expression

Sy, Xu; Wang, F; Gao, Wei; Wang, B; Jy, Yang; Yz, Huang; Zhang, L; Zheng, Fei; Ly, Guo; Jn, Wang; Jm, Tang

doi:10.1038/cgt.2013.70

Cited by 11 publications

(8 citation statements)

References 14 publications

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“…The results showed that the migration and invasion abilities of cells were gradually decreased in the interference group compared with the control group (21). Similar results were observed in gastric cancer tissues (19).…”

Section: Discussionsupporting

confidence: 73%

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RNAi-Mediated Knockdown of Skp2 Inhibits Human Bladder Cancer Proliferation and Invasion in T24 Cells

Xiong

et al. 2016

Iran Red Crescent Med J

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Background: Skp2 (S-phase kinase-associated protein 2 is overexpressed in many kinds of cancers, and is related to the occurrence and development of tumors. The molecular mechanism of Skp2 in the regulation of bladder cancer cell biological behavior after Skp2 expression knockdown, however, has remained unknown. Objectives: In our present studies (experimental cytobiological studies, we used an RNAi approach to knock down Skp2 expression, and studied its impact on cell proliferation and invasion of T24 cells. Materials and Methods:The expression of the Skp2 gene was knocked down by RNA interference (RNAi) in T24 cells. The transcription level of Skp2 was detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis. The expression of Skp2, caspase-3, caspase-8, caspase-9, and p27 (p27 Kip1 ) were measured by western blot assay. Cell proliferation and apoptosis were

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Section: Discussionsupporting

confidence: 73%

“…It has been widely used in experimental studies (17)(18)(19)(20). Zhang Bing used the method of RNA interference to reduce the expression of Skp2 protein in GBC-SD cells.…”

Section: Discussionmentioning

confidence: 99%

RNAi-Mediated Knockdown of Skp2 Inhibits Human Bladder Cancer Proliferation and Invasion in T24 Cells

Xiong

et al. 2016

Iran Red Crescent Med J

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“…meanwhile, SKP2 was found to determine poor prognosis in colorectal carcinoma (38). Xu et al (39) knocked down the expression of SKP2 using an adenovirus expressing SKP2 shRNA in SW480 cells. Their results showed that the knockdown of SKP2 blocked growth and induced cell apoptosis in the CRC cell line SW480.…”

Section: Possible Oncogenes Of Fbps Skp2 Fbxl20 β-Trcp In Crcmentioning

confidence: 99%

New insights into the mechanism of F-box proteins in colorectal cancer (Review)

Gong

Huo

2015

Oncology Reports

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Colorectal cancer (CRC) is one of the most common cancers worldwide with a high incidence and mortality rate. Integrative studies including systematic sequencing of colorectal tumors have provided an unprecedented insight into the molecular basis of CRC. Recently, evidence indicates that F-box proteins (FBPs) play a critical role in the oncogenesis, invasion, metastasis and prognostic assessment of CRC. Therefore, this review discusses the recent literature regarding the function and regulation of FBPs in the pathogenesis of CRC. Furthermore, we highlight that FBPs may represent an attractive therapeutic target for CRC.

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“…Skp2 serves a regulatory role in several processes including cell cycle regulation, growth, differentiation, proliferation, metastasis and apoptosis. Skp2 has also been reported to serve a role in drug resistance and is closely associated with the development of tumors, demonstrating a prognostic value in cancer (6–8). Skp2 is overexpressed in several types of human cancer, and it therefore targeting Skp2 may be a promising strategy for cancer treatment (9–11).…”

Section: Introductionmentioning

confidence: 99%

Effects of RNAi‑induced Skp2 inhibition on cell cycle, apoptosis and proliferation of endometrial carcinoma cells

et al. 2019

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The aim of the current study was to investigate the underlying mechanism of S-phase kinase associated protein 2 (Skp2) gene inhibition by lentivirus-mediated RNA interference (RNAi) on the cell cycle, apoptosis and proliferation of endometrial carcinoma HEC-1-A cells. A lentivirus shRNA vector targeting Skp2 was constructed and transfected into HEC-1-A cells. HEC-1-A cells transfected with a scramble sequence were used as negative controls. The mRNA and protein expression of Skp2, p27, cyclin D1 and caspase-3 were detected via reverse transcription-quantitative polymerase chain reaction and western blotting, respectively. The effects of Skp2 inhibition on the cell cycle, apoptosis and proliferation of HEC-1-A cells were detected using flow cytometry and a cell counting kit-8. Skp2 co-expression data was analyzed using Oncomine and TCGA databases. The positive recombinant viral clones were identified via PCR and confirmed via sequencing. The mRNA and protein expression of Skp2 were significantly decreased in HEC-1-A cells transfected with the lentiviral vectors compared with the negative control. In addition, there were no significant changes in the mRNA expression of p27 and cyclin D1; however, the protein levels of p27 and cyclin D1 were upregulated and downregulated, respectively, in HEC-1-A cells transfected with lentiviral vectors compared with negative controls. RNAi-induced Skp2 inhibition exerted an anti-proliferative effect by inducing cell cycle arrest, however cell apoptosis was not significantly affected. In the TCGA database, Skp2 expression positively associated with IGF2R, IGF2BP3, IGFBP1 and CCNF, while Skp2 expression negatively associated with IGF2, IGFBP6, IGFBP7 and IGFBP3. RNAi-induced Skp2 inhibition upregulated the protein expression of p27 and downregulated the protein expression of cyclin D1. The expression of Skp2 in endometrial cancer may therefore be regulated by the insulin-like growth factor 1 receptor signaling pathway.

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S-phase kinase-associated protein 2 knockdown blocks colorectal cancer growth via regulation of both p27 and p16 expression

Cited by 11 publications

References 14 publications

RNAi-Mediated Knockdown of Skp2 Inhibits Human Bladder Cancer Proliferation and Invasion in T24 Cells

RNAi-Mediated Knockdown of Skp2 Inhibits Human Bladder Cancer Proliferation and Invasion in T24 Cells

New insights into the mechanism of F-box proteins in colorectal cancer (Review)

Effects of RNAi‑induced Skp2 inhibition on cell cycle, apoptosis and proliferation of endometrial carcinoma cells

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