1989
DOI: 10.1128/mcb.9.3.1005
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S-phase-specific transcription regulatory elements are present in a replication-independent testis-specific H2B histone gene.

Abstract: The testis-specific H2B histone (TH2B) gene is expressed in pachytene spermatocytes during meiotic prophase I in the absence of any significant DNA synthesis. Unlike somatic histones, synthesis of testis-specific histones is not affected by inhibitors of DNA synthesis. A genomic rat TH2B gene was cloned by using a DNA fragment derived from TH2B cDNA as a probe. Expression of the cloned TH2B was investigated by gene transfer experiments. From these studies, we found that the 5' upstream region of the cloned TH2… Show more

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Cited by 39 publications
(45 citation statements)
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“…speculate that Sprm-1 might be involved in regulation of genes such as the testis-specific histones that contain octamer elements in their regulatory region (40) and are activated before meiosis in the male germ cell (41). However, we cannot rule out the possibility that Sperm-i protein is stored in the developing germ cell and serves a function at a later stage of spermatogenesis.…”
mentioning
confidence: 92%
“…speculate that Sprm-1 might be involved in regulation of genes such as the testis-specific histones that contain octamer elements in their regulatory region (40) and are activated before meiosis in the male germ cell (41). However, we cannot rule out the possibility that Sperm-i protein is stored in the developing germ cell and serves a function at a later stage of spermatogenesis.…”
mentioning
confidence: 92%
“…released cells) and cells remaining under blocked conditions were determined. Thus, as described by Hwang and Chae [30], the cell-cycle dependence of the individual promoter constructs could be expressed as the ratio between the normalized mean luciferase activity of the released cultures and the mean luciferase value of the corresponding blocked cultures. Table 1.…”
Section: Methodsmentioning
confidence: 99%
“…The following protease inhibitors were added to the homogenization buffer: leupeptin, 0.5 pg/ml; pepstatin, 0.7 pg/ml; aprotinin 1 pg/ml; bestatin 40 pg/ml [20]. Protein extracts were frozen in liquid nitrogen and stored at -70°C until use.…”
Section: Nuclear Extractsmentioning
confidence: 99%
“…Aphidicolin was used to block transfected HeLa cells at the transition from the G1 to the S phase of the cell cycle (following essentially the protocol of Hwang and Chae [20]). Cells were transfected with the individual CAT gene/promoter constructs.…”
Section: Aphidicolin Treatment Of Transfected Hela Cellsmentioning
confidence: 99%