S. R. K. Glanville

Edwards, I. E. S.

doi:10.1177/030751335604200114

Cited by 2 publications

(5 citation statements)

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“…compared with 5 or 10 mg/kg/day. This is in contrast to reports by Edwards et al (4,5), which showed that five oral doses of D0870 at 5 mg/kg at 48-h intervals were protective for treatment of C. albicans infection. For showed D0870 at 50 mg/kg to be superior to fluconazole.…”

Section: Methodscontrasting

confidence: 99%

“…No standards for susceptibility or resistance have been established for T. glabrata. Although more isolates will need to be examined, it appears from our in vivo studies that a D0870 MIC of 4 ,ug/ml may correspond to resistance of T. glabrata.…”

Section: Methodsmentioning

confidence: 98%

“…Combination therapy with amphotericin B (3 mg/kg/day) and D0870 (25 mg/kg/day) was significantly better than either antifungal agent alone in reducing the isolate 1 CFU burden in kidney tissue. D0870, a new triazole, has exhibited excellent antifungal activity against systemic murine infections with C. neoformans and C. albicans in both kidney and brain tissue of normal and immunocompromised mice (4). D0870 is also active in vitro against fluconazole-resistant C. albicans (4).…”

Section: Methodsmentioning

confidence: 99%

“…D0870, a new triazole, has exhibited excellent antifungal activity against systemic murine infections with C. neoformans and C. albicans in both kidney and brain tissue of normal and immunocompromised mice (4). D0870 is also active in vitro against fluconazole-resistant C. albicans (4). In vivo, D0870 is reported to be 5-to 25-fold more potent than fluconazole for treatment of murine vaginal and systemic infections (3) and 2-to 7-fold superior to fluconazole in normal mice versus 3-to 90-fold superior in mice immunosuppressed with either cortisone or cyclophosphamide (14).…”

Section: Methodsmentioning

confidence: 99%

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Treatment of disseminated Torulopsis glabrata infection with DO870 and amphotericin B

Atkinson

Bocanegra

Colombo

et al. 1994

Antimicrob Agents Chemother

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One day prior to challenge, the organisms were subcultured to 50 ml of brain heart infusion (BHI) broth (BBL, Cockeysville, Md.). The fungi were incubated on a shaker at 37°C overnight, washed twice with 0.9% NaCl, quantified with a hemacytometer, and adjusted to the desired concentration in normal saline. The counts were verified by determining CFU counts with 10-fold serial dilutions in 0.9% NaCl. Each mouse was infected with 1.1 x 108 to 7 x 108 CFU/ml in the lateral tail vein given in a 0.2-ml volume. The T. glabrata cells were agitated between each injection to achieve a uniform suspension.Activity in vitro. The in vitro susceptibility studies were performed by the broth macrodilution technique proposed by the National Committee for Clinical Laboratory Standards (7). The medium was RPMI 1640 with glutamine, buffered by MOPS (morpholinepropanesulfonic acid) at pH 7.0 (American Bioganics, Niagara Falls, N.Y.). The inoculum was prepared from 24-h Sabouraud dextrose agar (Difco Laboratories, Detroit, Mich.) subcultures of T. glabrata incubated at 35°C. Colonies of T. glabrata were suspended in 5 ml of sterile distilled water, and the cell density was adjusted with a spectrophotometer to a 0.5 McFarland standard at a 530-nm wavelength. The working suspension was a 1:100 dilution of the organisms followed by a 1:20 dilution of the suspension with RPMI broth medium. A volume of 0.9 ml of the adjusted solution was dispensed into tubes containing 0.1 ml of 10 times the concentrations of the drugs to be tested. The test tubes were incubated at 35°C for 48 h.

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Section: Methodscontrasting

confidence: 99%

Section: Methodsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Treatment of disseminated Torulopsis glabrata infection with DO870 and amphotericin B

Atkinson

Bocanegra

Colombo

et al. 1994

Antimicrob Agents Chemother

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“…We have re-examined this problem in vivo and present evidence that cyclic AMP may be involved in the activation of cerebral phosphorylase and the subsequent glycogenolysis induced by certain biogenic amines. A preliminary 565 account of this work has been presented to the British Pharmacological Society (EDWARDS et al, 1973).…”

mentioning

confidence: 99%

IN VIVO CHANGES OF CEREBRAL CYCLIC ADENOSINE 3′,5′‐MONOPHOSPHATE INDUCED BY BIOGENIC AMINES: ASSOCIATION WITH PHOSPHORYLASE ACTIVATION

Edwards

Nahorski

Rogers

1974

Journal of Neurochemistry

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The intravenous injection of adrenaline, isoprenaline and histamine to 4-6-day-old chicks resulted in a rapid increase in the cyclic AMP content of cerebral hemispheres that had been removed and frozen within 0.5 s using a freeze-blowing technique. Noradrenaline, dopamine, adenosine, 5-HT and acetylcholine did not significantly alter the nucleotide concentration in uiuo. Addition of adrenaline, isoprenaline and histamine to incubated chick cerebral cortex slices also increased the cyclic AMP content of the tissue. Noradrenaline was considerably less potent than these amines and adenosine was ineffective.Low phosphorylase a levels (16 per cent of total activity) were observed in instantaneously frozen cerebral hemispheres of untreated chicks. The injection of adrenaline, isoprenaline and histamine resulted in a rapid conversion of phosphorylase b to a and a significant fall in tissue glycogen. Administration of noradrenaline was without effect on the relative forms of phosphorylase and also failed to influence cerebral glycogen. Phosphorylase activation was not observed in chick cerebral slices under conditions prod Ycing large increases in cyclic AMP.It is suggested that in uiuo phosphorylase -activation and subsequent glycogenolysis may occur, at least in part, in glia and that these cells may be damaged during preparation of cerebral slices. The results provide evidence of a metabolic role for cyclic AMP in cerebral tissue.

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S. R. K. Glanville

Cited by 2 publications

References 0 publications

Treatment of disseminated Torulopsis glabrata infection with DO870 and amphotericin B

Treatment of disseminated Torulopsis glabrata infection with DO870 and amphotericin B

IN VIVO CHANGES OF CEREBRAL CYCLIC ADENOSINE 3′,5′‐MONOPHOSPHATE INDUCED BY BIOGENIC AMINES: ASSOCIATION WITH PHOSPHORYLASE ACTIVATION

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